The effects of photobiomodulation on human dermal fibroblasts in vitro: A systematic review

Tripodi, Nicholas; Corcoran, Daniel; Antonello, Peta; Balic, Nicholas; Caddy, Darcy; Knight, Aidan; Meehan, Casey; Sidiroglou, Fotios; Fraser, Sarah; Kiatos, Dimitrios; Husaric, Maja; Apostolopoulos, Vasso; Feehan, Jack

doi:10.1016/j.jphotobiol.2020.112100

Cited by 30 publications

(25 citation statements)

References 82 publications

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“…Fibroblasts were used in this study due to the fact presented as the major constituent of connective tissue, being the most predominant cell type in the periodontal ligament and the largest producer of collagen, elastin, glycosaminoglycans and glycoproteins 30,31 . Intracanal medications when in contact with the periapical tissues cause an inflammatory response and the LPBM could provide an enhanced cellular response in the affected area.…”

Section: Discussionmentioning

confidence: 99%

Laser photobiomodulation effect on fibroblasts viability exposed to endodontic medications

Lima

Chisini²,

Conde³

et al. 2021

Braz. J. Oral Sci.

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Aim: The literature has not yet reported investigations about the effect of laser photobiomodulation (LPBM) over the cytotoxicity of drugs for endodontic treatments. Thus, the aim of this study was to evaluate, in vitro, the effect of the association between LPBM and intracanal medications on fibroblasts viability in different exposure times. Methods: Calcium hydroxide (Ca(OH)2) and iodoform (IO) were used pure or associated to LPBM. Eluates of medications were prepared and placed in contact with the cells in three different periods: 24h, 48h and 72h. Laser irradiation (emitting radiation λ 660nm, power density of 10mW, energy density of 3 J/cm²) has been performed in two sessions within a six hour interval, for 12s per well. After each experimental time, the colorimetric assay (MTT) has been performed. Statistical analysis was applied for Mann-Whitney test with 5% α error admitted test. Results: At 24h, the use of LPBM did not increase cell viability while after 72h cell proliferation was stimulated in the group without medications. LPBM application did not increase cell viability in Ca(OH)2 group and IO at any tested time. Ca(OH)2 cytotoxicity at 24h was higher than iodoform, while at 72h not difference was observed. Therefore, after 72 hours was no statistical difference between the IO and Ca(OH)2 groups. Conclusion: LPBM was able to increase cell viability in 72h in the group without medication, although no improvement was observed in the other groups. Thus, LPBM was not able to reduce the cytotoxic effects of the materials on fibroblasts in vitro.

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Section: Discussionmentioning

confidence: 99%

Laser photobiomodulation effect on fibroblasts viability exposed to endodontic medications

Lima

Chisini²,

Conde³

et al. 2021

Braz. J. Oral Sci.

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“…This is the first phase in wound healing and is characterised by a vascular response [34]. It involves the constriction of blood vessels immediately after injury to restrict blood flow to the injured tissue [35].…”

Section: Haemostatic Phasementioning

confidence: 99%

“…The second stage in wound healing, inflammation, is characterised by a cellular response, which is highlighted by the influx of white blood cells, mainly neutrophils and macrophages [3,35]. It is initiated when there is inhibition of local lymphatic function due to the extravasation of fluid to extracellular spaces as a result of the dilation of injured blood vessels [34]. This causes the cardinal signs associated with inflammation, namely heat, redness, swelling, and pain, at the location of the lesion.…”

Section: Inflammatory Phasementioning

confidence: 99%

“…The third stage in wound healing, the proliferative phase, features three specific stages, namely re-epithelialisation, neovascularisation, and granulation [4,34]. It is characterised by angiogenesis and the formation of collagen and extracellular matrix (ECM) complexes critical for proper repair [1].…”

Section: Proliferative Phasementioning

confidence: 99%

“…PBM uses low-intensity light over the injury site to reduce pain and inflammation and activate tissue repair [8]. It is a low-risk, non-invasive, pain-free, and cost-effective medical therapy for the effective management of conditions, such as non-healing chronic wounds [34,84]. There are well-entrenched reports on the range of clinical benefits induced by PBM, from the treatments on non-healing chronic wounds, ulcers, burn wounds, chronic pain, and immune modulation, to dermatological conditions, scarring, and athletic performance enhancements [7,[85][86][87].…”

Section: Photobiomodulation (Pbm)mentioning

confidence: 99%

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Cellular Signalling and Photobiomodulation in Chronic Wound Repair

Leyane

Jere

Houreld

2021

IJMS

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Photobiomodulation (PBM) imparts therapeutically significant benefits in the healing of chronic wounds. Chronic wounds develop when the stages of wound healing fail to progress in a timely and orderly frame, and without an established functional and structural outcome. Therapeutic benefits associated with PBM include augmenting tissue regeneration and repair, mitigating inflammation, relieving pain, and reducing oxidative stress. PBM stimulates the mitochondria, resulting in an increase in adenosine triphosphate (ATP) production and the downstream release of growth factors. The binding of growth factors to cell surface receptors induces signalling pathways that transmit signals to the nucleus for the transcription of genes for increased cellular proliferation, viability, and migration in numerous cell types, including stem cells and fibroblasts. Over the past few years, significant advances have been made in understanding how PBM regulates numerous signalling pathways implicated in chronic wound repair. This review highlights the significant role of PBM in the activation of several cell signalling pathways involved in wound healing.

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e‐Bandage: Exploiting Smartphone as a Therapeutic Device for Cutaneous Wound Treatment

Yu,

Yi,

Lin

et al. 2024

Advanced Intelligent Systems

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Near‐infrared (NIR) radiation has demonstrated significant promise in skin tissue engineering and wound healing. However, the high cost and operational complexity associated with current specialized apparatuses have hindered their practical household use. In response to these issues, a flexible, battery‐free, wireless sensor–actuator NIR therapy system, offering real‐time physiological monitoring and active treatment to cutaneous wounds, is developed. The wound management system can be wirelessly powered, and transmits data to any near‐field communication‐compatible smartphone. The therapeutic prowess of our system for wound closure is computationally and experimentally demonstrated by promoting epithelial migration and modulating inflammation. Moreover, a novel feature is reported: the smartphone‐enabled system can precisely predict the wound stage via a temperature sensor pair, which can provide an effective timely intervention strategy for NIR wound therapy. Studies based on rat wound model successfully confirm the efficacy of the proposed strategy, showcasing a remarkable 30% enhancement in wound closure compared to control groups. The user‐friendly, effective wound management platform promises a revolutionary step toward accessible and efficient at‐home wound care.

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The effects of photobiomodulation on human dermal fibroblasts in vitro: A systematic review

Cited by 30 publications

References 82 publications

Laser photobiomodulation effect on fibroblasts viability exposed to endodontic medications

Laser photobiomodulation effect on fibroblasts viability exposed to endodontic medications

Cellular Signalling and Photobiomodulation in Chronic Wound Repair

e‐Bandage: Exploiting Smartphone as a Therapeutic Device for Cutaneous Wound Treatment

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