The effects of pollution on the vitamins A, E, C, β-carotene contents and oxidative stress of the freshwater crayfish, Astacus leptodactylus

Barim, Özden; Karatepe, Mustafa

doi:10.1016/j.ecoenv.2009.08.002

Cited by 38 publications

(47 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The supernatant was collected and stored at −80 °C. The hepatic MDA level was measured (Barim and Karatepe, 2010) using the fully automatic HPLC (Shimadzu, Kyoto, Japan) equipped with a pump (LC‐20AD), an ultraviolet‐visible detector (SPD‐20A), an inertsil ODS‐3 C18 column (250 × 4.6 mm, 5 m), a column oven (CTO‐10ASVP), an autosampler (SIL‐20A), a degasser unit (DGU‐20A5), and a computer system with LC solution Software (Shimadzu). Total SOD activity was attained based on the amount of enzyme required to inhibit the rate of formazan dye formation by 50% (Spitz and Oberley, 1989).…”

Section: Methodsmentioning

confidence: 99%

Resveratrol protects quail hepatocytes against heat stress: modulation of the Nrf2 transcription factor and heat shock proteins

Şahin

Orhan

Akdemir

et al. 2011

Animal Physiology Nutrition

103

View full text Add to dashboard Cite

In the present study, the effects of dietary resveratrol on the induction of heat shock proteins, transcription factors and antioxidative enzyme system in liver of quails under heat stress were investigated. A total of 180 (55-day-old) female Japanese quails (Coturnix coturnix japonica) were reared either at 22 °C for 24 h/day (thermoneutral, TN) or 34 °C for 8 h/day (heat stress, HS; 09:00-17:00 hours) for 12 weeks. Birds in both environments were randomly fed one of three diets: basal diet and basal diet added with either 200 or 400 mg of resveratrol per kg of diet. The results showed that exposure to high ambient temperature induced decreases in feed intake, egg production, and hepatic superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities but increases in hepatic malondialdehyde (MDA) concentrations (p < 0.001). Liver Hsp70, Hsp90 and NF-κB expression was greater while Nrf2 expression was lower for quails reared under the heat stress than for those reared under the TN environment (p < 0.0001). There were linear increases in feed intake, egg production, hepatic SOD, CAT, and GSH-Px activities as well as Nrf2 expression, but linear decreases in hepatic MDA concentrations and Hsp70, Hsp90, and NF-κB expressions with increasing supplemental resveratrol level (p < 0.0001). Two-way treatment interactions revealed that the degree of restorations in all response variables was more notable under the high ambient temperature than that of the TN environment as dietary resveratrol concentration was increased. The results of the present study suggest that supplemental resveratrol reduces oxidative stress in heat-stressed quails through modulating the hepatic heat shock proteins and nuclear transcription factors.

show abstract

Section: Methodsmentioning

confidence: 99%

Resveratrol protects quail hepatocytes against heat stress: modulation of the Nrf2 transcription factor and heat shock proteins

Şahin

Orhan

Akdemir

et al. 2011

Animal Physiology Nutrition

103

View full text Add to dashboard Cite

show abstract

“…Although P. leptodactylus is not considered a model organism, it is one of the most studied species among decapods with about 143 published papers regarding its physiology [1], [36], [37], [38], its resistance to different types of pollution and stress conditions [39], [40], [41] and concerning the cHH and its variety of functions [42], [43], [44], [45]. Unfortunately this variety of studies does not reflect the fast progress of genomics and transcriptomics.…”

Section: Introductionmentioning

confidence: 99%

Application of D-Crustacean Hyperglycemic Hormone Induces Peptidases Transcription and Suppresses Glycolysis-Related Transcripts in the Hepatopancreas of the Crayfish Pontastacus leptodactylus — Results of a Transcriptomic Study

et al. 2013

View full text Add to dashboard Cite

The crustacean Hyperglycemic Hormone (cHH) is a neuropeptide present in many decapods. Two different chiral isomers are simultaneously present in Astacid crayfish and their specific biological functions are still poorly understood. The present study is aimed at better understanding the potentially different effect of each of the isomers on the hepatopancreatic gene expression profile in the crayfish Pontastacus leptodactylus, in the context of short term hyperglycemia. Hence, two different chemically synthesized cHH enantiomers, containing either L- or D-Phe3, were injected to the circulation of intermolt females following removal of their X organ-Sinus gland complex. The effects triggered by the injection of the two alternate isomers were detected after one hour through measurement of circulating glucose levels. Triggered changes of the transcriptome expression profile in the hepatopancreas were analyzed by RNA-seq. A whole transcriptome shotgun sequence assembly provided the assumedly complete transcriptome of P. leptodactylus hepatopancreas, followed by RNA-seq analysis of changes in the expression level of many genes caused by the application of each of the hormone isomers. Circulating glucose levels were much higher in response to the D-isoform than to the L-isoform injection, one hour from injection. Similarly, the RNA-seq analysis confirmed a stronger effect on gene expression following the administration of D-cHH, while just limited alterations were caused by the L-isomer. These findings demonstrated a more prominent short term effect of the D-cHH on the transcription profile and shed light on the effect of the D-isomer on specific functional gene groups. Another contribution of the study is the construction of a de novo assembly of the hepatopancreas transcriptome, consisting of 39,935 contigs, that dramatically increases the molecular information available for this species and for crustaceans in general, providing an efficient tool for studying gene expression patterns in this organ.

show abstract

“…External stressors, particularly xenobiotics, may lead to the formation of excessive quantities of reactive oxygen species (ROS) and oxidative stress damage, such as gene transcription modification, lipid peroxidation and change the morphology and the physiology of fish tissues (Dautremepuits et al 2009). To prevent the damaging effect of free radicals, cells and tissues may respond using enzymatic and non-enzymatic activities (Barim and Karatepe 2010). Retinoids (vitamin A) are part of the latter group and these compounds have been found to be modulated by environmental pollutants in fish (Zhang et al 2002;Barim and Karatepe 2010).…”

Section: Introductionmentioning

confidence: 99%

“…To prevent the damaging effect of free radicals, cells and tissues may respond using enzymatic and non-enzymatic activities (Barim and Karatepe 2010). Retinoids (vitamin A) are part of the latter group and these compounds have been found to be modulated by environmental pollutants in fish (Zhang et al 2002;Barim and Karatepe 2010). Surface water contaminants such as pesticides (e.g., atrazine, metolachlor) have been reported to modify the retinoic signalling pathway (Bérubé et al 2005;Novák et al 2008) and to induce organism malformations (e.g., skeletal deformities) in fish larvae (Inoue et al 2010;Yutaka et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Integrated spatial health assessment of yellow perch (Perca flavescens) populations from the St. Lawrence River (QC, Canada), part B: cellular and transcriptomic effects

Bruneau

Landry

Giraudo

et al. 2016

Environ Sci Pollut Res

View full text Add to dashboard Cite

Multi-biological level assessments have become great tools to evaluate the health of aquatic ecosystems. Using this approach, a complementary study was designed to evaluate the health of yellow perch (Perca flavescens) populations in the St. Lawrence River (Quebec, Canada). In the present study, stress responses were compared at the transcriptomic, cellular, and tissue levels in yellow perch collected at six sites along the river: Lake St. François, Lake St. Louis (north and south), Beauregard Island and Lake St. Pierre (north and south). These results complement the physiological and chemical parameters as well as pathogen infection investigated in a companion paper published in the present issue. Thiobarbituric acid reactive substance (TBARS) analyses indicated the presence of oxidative stress in fish collected in the southern part of Lake St. Louis and at the downstream sites of Lake St. Pierre. High lipid peroxidation levels were found in the muscle of yellow perch caught at Beauregard Island, located downstream of the Montreal's wastewater treatment plant, suggesting an impact of the municipal effluent on redox homeostasis. Transcriptomic results indicated the down-regulation of genes related to lipid, glucose, and retinoid in southern Lake St. Pierre as well as a decrease in retinoid storage. Overall, biochemical and molecular markers indicated that the health status of yellow perch followed a decreasing gradient from upstream to downstream of the St. Lawrence River. This gradient is representative of the cumulative negative impacts of human activities on water and habitat quality along the river.

show abstract

The effects of pollution on the vitamins A, E, C, β-carotene contents and oxidative stress of the freshwater crayfish, Astacus leptodactylus

Cited by 38 publications

References 18 publications

Resveratrol protects quail hepatocytes against heat stress: modulation of the Nrf2 transcription factor and heat shock proteins

Resveratrol protects quail hepatocytes against heat stress: modulation of the Nrf2 transcription factor and heat shock proteins

Application of D-Crustacean Hyperglycemic Hormone Induces Peptidases Transcription and Suppresses Glycolysis-Related Transcripts in the Hepatopancreas of the Crayfish Pontastacus leptodactylus — Results of a Transcriptomic Study

Integrated spatial health assessment of yellow perch (Perca flavescens) populations from the St. Lawrence River (QC, Canada), part B: cellular and transcriptomic effects

Contact Info

Product

Resources

About