The effects of resveratrol on porcine oocyte in vitro maturation and subsequent embryonic development after parthenogenetic activation and in vitro fertilization

Kwak, Seong-Sung; Cheong, Seung-A; Jeon, Yubyeol; Lee, Eunsong; Choi, Kyung‐Chul; Jeung, Eui‐Bae; Hyun, Sang‐Hwan

doi:10.1016/j.theriogenology.2012.01.024

Cited by 149 publications

(157 citation statements)

References 72 publications

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“…Based on our observation it was revealed that although resveratrol treatment at the concentrations of 0.25 and 0.5 μM produce optimum effect the higher concentration of this (5 μM) has detrimental impact. This is in line with the earlier reports of dose-dependent effect exerted by resveratrol in cell proliferation [20,39] as well as mammalian embryonic development [21,24]. Supplementation of antioxidants in culture medium influences oocyte maturation and embryonic development.…”

Section: Discussionsupporting

confidence: 92%

“…SIRT1 stimulates mitochondrial biogenesis through deacetylation and activation of PGC-1α [10,36], a key controller of mitochondrial biogenesis that coactivates the nuclear respiratory factors (NRF-1 and NRF-2), which induce the transcription of genes involved in mitochondrial biogenesis [37]. The beneficial effect of resveratrol on porcine oocyte maturation and embryonic development has been reported recently [21,24]. However, no report is available on the effect of resveratrol treatment on embryonic development in goat.…”

Section: Discussionmentioning

confidence: 99%

“…So it may be inferred that supplementation of resveratrol in the IVM medium promotes cytoplasmic maturation of oocytes and increased intracellular GSH level contributed to the higher embryonic development following PA and HMC. Previously, two separate studies to find out the effect of resveratrol treatment on porcine oocyte development reported that resveratrol at the concentration of 0.5 μM [24] and 2.0 μM [21] increased the blastocyst yield following PA and IVF. The later also reported increased intracellular GSH concentration at 0.5 and 2.0 μM resveratrol-treated groups.…”

Section: Discussionmentioning

confidence: 99%

“…Compounds with antioxidative properties such as β-mercatoethanol (β-ME), cysteine [6], cysteamine [30], L-carnitine [44] that augment intracellular glutathione level have been added in IVM as well as in vitro culture (IVC) media. Recently, supplementation of Resveratrol (3,4′, 5-trihydroxystilbene), a naturally occurring phytoalexin, has been found to have beneficial impact on porcine IVM and embryonic development [21]. Resveratrol is produced by the interaction of plants with a microorganism present in the roots of Polygonum cuspidatum , Vitis vinifera , red wines, and mulberries and protects these plants against fungal and bacterial infections [23].…”

Section: Introductionmentioning

confidence: 99%

“…However, our knowledge on effects of resveratrol on livestock oocyte maturation and embryonic developmental competence is inadequate. It has been observed that resveratrol supplementation at lower concentration improved developmental potential of oocytes [21,24]. Here we investigated the effects of resveratrol on goat IVM and subsequent embryonic development after parthenogenetic activation (PA) and SCNT though hand-made cloning (HMC).…”

Section: Introductionmentioning

confidence: 99%

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Resveratrol treatment during goat oocytes maturation enhances developmental competence of parthenogenetic and hand-made cloned blastocysts by modulating intracellular glutathione level and embryonic gene expression

Mukherjee

Malik

Saha

et al. 2013

J Assist Reprod Genet

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Purpose The aim of the present study was to determine whether supplementation of resveratrol, a stilbenoid antioxidant with therapeutic significance, influences goat (Capra hircus) oocyte maturation and subsequent embryonic development and expression of apoptosis and early embryonic development-related genes. Methods Five different concentrations of resveratrol (0.1, 0.25, 0.5, 2.0 and 5.0 μM) were used in in vitro maturation (IVM) medium. Cell tracker blue and 2′,7′-dichlorodihydrofluorescein diacetate (H 2 DCFDA) fluorescent stains were used to assay intracellular glutathione and reactive oxygen species levels in mature oocytes. Parthenogenetic activation and hand-made cloning were performed to check the developmental potential following resveratrol treatment. We used quantitative real-time PCR to analyze embryonic gene expression. Result Compared to control, no significant improvement was observed in nuclear maturation in resveratrol-treated groups and at 5.0 μM concentration maturation rate decreased significantly (P <0.05). But resveratrol treatment at the concentrations of 0.25, 0.5 μM significantly reduced intracellular ROS, and increased GSH concentrations. Oocytes treated with 0.25, 0.5 μM resveratrol when subsequently used for PA and HMC, higher extent of blastocyst yields were observed. Expression analysis of proapoptotic (Bax) gene in mature oocytes, cumulus cells, and HMC-derived blastocysts revealed lesser transcript abundances in various resveratrol-treated groups., however no change in the same was observed for antiapoptotic gene (Bcl2). Differential expression of genes associated with developmental competence and nuclear reprogramming was also observed in HMC-derived blastocysts. Conclusion Our results show that resveratrol treatment at optimum concentrations (0.25 and 0.5 μM) during IVM produced beneficial microenvironment within oocytes by increasing the intracellular GSH, decreasing ROS level and this in turn, stimulated embryonic development and regulated gene expression.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Resveratrol treatment during goat oocytes maturation enhances developmental competence of parthenogenetic and hand-made cloned blastocysts by modulating intracellular glutathione level and embryonic gene expression

Mukherjee

Malik

Saha

et al. 2013

J Assist Reprod Genet

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Sonic hedgehog signaling mediates resveratrol to improve maturation of pig oocytes in vitro and subsequent preimplantation embryo development

Lee

Jin

Taweechaipaisankul

et al. 2018

Journal Cellular Physiology

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The beneficial effects of resveratrol on in vitro maturation (IVM) have been explained mainly by indirect antioxidant effects and limited information is available on the underlying mechanism by which resveratrol acts directly on porcine cumulus oocyte complexes (COCs). Recently, several studies reported that sonic hedgehog (SHH) signaling mediates resveratrol to exert its biological activities. Furthermore, SHH is an important signaling molecule for follicle development, oocyte maturation, and embryo development. Therefore, to elucidate the relationship between resveratrol and SHH signaling, we designed three groups: (i) control; (ii) resveratrol; and (iii) resveratrol with cyclopamine (SHH signaling inhibitor). We evaluated the effects of these agents on cumulus expansion, oocyte maturation, embryo development after parthenogenetic activation, expression levels of mRNAs in cumulus cells, oocytes and blastocysts, and protein expression in COCs. Resveratrol significantly increased the proportion of COCs exhibiting complete cumulus expansion (degree 4), oocyte nuclear maturation, cleavage and blastocyst formation rates and total cell numbers, which were blocked in the presence of cyclopamine. At the same time, a significant increase in the expression levels of mRNAs related to cumulus expansion, oocyte maturation and SHH signaling-related mRNAs and proteins from the resveratrol treatment group was also inhibited by simultaneous addition of cyclopamine. In conclusion, our results indicate that SHH signaling mediates resveratrol to improve porcine cumulus expansion, oocyte maturation, and subsequent embryo development.

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Age-associated changes in gene expression and developmental competence of bovine oocytes, and a possible countermeasure against age-associated events

et al. 2013

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In general, maternal age affects the quality of oocytes and embryos. The present study aimed to examine the features and age-associated gene expression profiles of bovine oocytes and embryos as well as to discover possible countermeasures against age-associated events. Comprehensive gene expression assays of germinal vesicle and metaphase II (MII)-stage oocytes and 8- to 16-cell-stage embryos were conducted using next-generation sequencing technology. The gene expression profiles of aged cows showed high expression of genes related to oxidative phosphorylation, eIF4 and p70S6K signaling, and mitochondrial dysfunction in MII-stage oocytes. Oocytes derived from aged cows, compared with those derived from their younger counterparts, exhibited high levels of abnormal fertilization and blastocysts with low total cell numbers. Levels of reactive oxygen species (ROS) and SIRT1 were higher in in vitro-matured oocytes derived from aged cows than in those derived from their younger counterparts. Supplementation of maturation medium with N-acetyl-cysteine (NAC), but not resveratrol, reduced the levels of ROS in the oocytes derived from cows of both age groups; however, resveratrol, but not NAC, improved the fertilization ratio. Conversely, EX 527, an inhibitor of SIRT1, increased the ratio of abnormal fertilization. In conclusion, gene expression profiles of oocytes and embryos derived from aged cows differ from those of oocytes and embryos derived from young cows; in particular, oocytes derived from aged cows show protein and mitochondrial dysfunction. In addition, activation of SIRT1 in oocytes may be a potential countermeasure against age-associated events in oocytes derived from aged cows.

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The effects of resveratrol on porcine oocyte in vitro maturation and subsequent embryonic development after parthenogenetic activation and in vitro fertilization

Cited by 149 publications

References 72 publications

Resveratrol treatment during goat oocytes maturation enhances developmental competence of parthenogenetic and hand-made cloned blastocysts by modulating intracellular glutathione level and embryonic gene expression

Resveratrol treatment during goat oocytes maturation enhances developmental competence of parthenogenetic and hand-made cloned blastocysts by modulating intracellular glutathione level and embryonic gene expression

Sonic hedgehog signaling mediates resveratrol to improve maturation of pig oocytes in vitro and subsequent preimplantation embryo development

Age-associated changes in gene expression and developmental competence of bovine oocytes, and a possible countermeasure against age-associated events

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