The effects of resveratrol on cyclooxygenase-1 and cyclooxygenase-2 mRNA and protein levels in diabetic rat kidneys

Abstract

Cyclooxygenase (COX), which have the isoforms of COX-1 and COX-2, is the key enzyme of prostaglandins biosynthesis. Especially, COX-2 is induced in inflammatory disease such as Diabetes Mellitus (DM). Resveratrol (RSV), a natural antioxidant, has a beneficial role in prevention of inflammatory disease. We investigated the changes of COX-1 and COX-2 mRNA expression and protein level in diabetic rat kidney after RSV treatment. Three months-old, 44 Wistar albino male rats, which were divided into six groups such as control group, sodium citrate buffer (sham control) group, diabetic group (DM), Dimethyl Sulfoxide induced control group, RSV treated sham control group (RSV) and RSV treated diabetic group (DM + RSV) were used for the study. Experimental diabetes was induced by intraperitoneal injection of 55 mg/kg Streptozotocin. After the induction of chronic diabetes 10 mg/kg per day RSV was administered intraperitoneally for 4 weeks. In this study. RSV has no significant effect on COX-1 mRNA expression in diabetic rat kidney (P > 0.05). Immunohistochemical study showed that COX-1 expression was slightly inhibited in RSV group and was not significantly supressed in DM + RSV group. When comparing control and treated groups, there were no significant differences in COX-2 mRNA or protein levels (P > 0.05). In conclusion, our results indicate that resveratrol do not significantly affect COX gene and protein expression. Therefore, different therapy strategies such as combination with other antidiabetic drugs may tried in STZ induced animal model for reducing diabetic symptoms and altering COX-1 and COX-2 mRNA or protein levels.

Human glomerular endothelium: interplay among glucose, free fatty acids, angiotensin II, and oxidative stress

“…However, our studies also demonstrate further increase in COX-2 expression after highglucose exposure and ANG II. Moreover, the glomerular and cortical expression of COX-2 is increased in animal models of diabetes, suggesting that COX-2 plays an important role in the pathogenesis of diabetic nephropathy (23,24,45).…”

Melatonin, quercetin and resveratrol attenuates oxidative hepatocellular injury in streptozotocin-induced diabetic rats

“…The anti-cancer, antioxidant, antifungal infection, anti-inflammatory activity, antiplatelet activity, pro-apoptotic and cardioprotective properties of resveratrol have been well established recently. [12][13][14][15] The purpose of the this study was to investigate the effects of melatonin, quercetin and resveratrol on hepatocellular injury in STZ-induced diabetes.…”

The effects of resveratrol on cyclooxygenase-1 and -2, nuclear factor kappa beta, matrix metalloproteinase-9, and sirtuin 1 mRNA expression in hearts of streptozotocin-induced diabetic rats

“…Although RSV is thought to be a specific inhibitor of COX-1 (Szewczuk et al, 2004), at high concentrations it has been reported to reduce basal COX-2 activity in vitro (Jang et al, 1997). Our previous study demonstrated that RSV reduced COX-2 and especially COX-1 mRNA level but there were no statistically significant differences between DM and DM+RSV groups in STZ-induced DM rat kidney tissue (P > 0.05) (Yar et al, 2010). Similarly, RSV reduced the COX-1 mRNA level in STZ-induced DM rat heart tissues in the present study.…”

“…All animals were acclimatized for a minimum period of 1 week prior to the beginning of the study. Forty-four 3-month-old, Wistar albino male rats were used for the study as described previously (Yar et al, 2010). Animals were randomly divided into 6 experimental groups [group I: nondiabetic rats used as a control group (N = 6); group II: sodium citrate buffer control group (sham control; N = 7); group III: STZ-induced diabetic group (DM; N = 9); group IV: DMSO-induced control group (DMSO; N = 6); group V: RSV-treated sham control group (RSV; N = 7); group VI: RSV-treated diabetic group (DM+RSV; N = 9)].…”

“…Rats with blood glucose concentrations ≥300 mg/ dL were considered as diabetic and used for the study. During the experiment, blood glucose levels were verified eight times (from the 3rd to the 60th day after the beginning of treatment) (Yar et al, 2010).…”