The effects of rice seed dressing with Paenibacillus yonginensis and silicon on crop development on South Korea’s reclaimed tidal land

Abstract

a b s t r a c tA field trial to investigate the effects of plant growth promoting bacteria (PGPB) Paenibacillus yonginensis (DCY84 T ) and/or SiO 2 seed coating on rice growth (Oryza sativa L.) was carried out on reclaimed tidal land in Taean County in South Korea. The field test was performed twice between May-October 2014 and May-October 2015, in a randomized complete block (RCB) design with three replications. Treatments consisted of: Mock, DCY84T -treated seeds, SiO 2 -coated seeds and DCY84 T -SiO 2 -treated seeds and each treatment area covered approximately 300 m 2 . During the early developmental period of rice seedlings, the SiO 2 coating without DCY84T led to the most favorable 30 DAS rice seedling parameters. Moreover, the combination of DCY84 T and SiO 2 treatments resulted in 2-fold greater fresh and dry weights of 60 DAS rice seedlings compared to Mock seedlings. DCY84T and SiO 2 , both individually and together, produced a greater grain yield and a greater total yield; specifically, DCY84T and SiO 2 treatments yielded a 73% and 70% increase in mass compared to Mock plants, respectively. Rice treated with both DCY84 T and SiO 2 treatment contained the highest amount of Al, Fe, Ca and Mg, which were 54%, 169%, 42% and 67% higher than the Mock rice, respectively. Remarkably, DCY84T treatment had the most phosphate [P], potassium [K] and total nitrogen [T-N]. DCY84T and/or SiO 2 treatment highly increased the whole kernel percentage. Thus, lower its broken kernel percentage to 9.60-24.58%. The protein content of the grain with both treatments was 7.2%, which was greater than that of the Mock grain (6.0%). The content of chlorophyll a, b and carotinoid in the rice leaves which were treated with silica and DCY84 has increased more than the mock without treatment. After harvest, the GABA content of brown rice was increased to 1.9-fold (2014), 1.5 fold (2015) compared to mock grain, respectively. Overall, DCY84T treatment and SiO 2 coating can be useful methods for promoting growth of rice under conditions of saline stress. Results from other laboratory trials and greenhouse experiments are also provided.

Silicification of Root Tissues

“…Since the root is in tight contact with other microorganisms in the soil, the role of plant growth promoting bacteria (PGPB) as well as mycorrhiza, and their interaction with Si and abiotic stress factors should be also considered. This does not seem to be an intensively studied topic, however, some reports indicated that Si applied together with PGPB may help to overcome the drought stress or improve the vigor of plants grown in saline conditions [138,139].…”

Complete genome sequence of Paenibacillus yonginensis DCY84T, a novel plant Symbiont that promotes growth via induced systemic resistance

“…Silica zeolite complex and two treatments with strain DCY84 T resulted in the highest head rice levels (86.8%) compared to a one-time treatment of DCY84 T (67.9%), and without strain DCY84 T treatment (46.4%). The PGPR treatment enhanced head rice levels by 40.4% [ 16 ]. Strain treatment also enhanced nitrogen uptake and increased levels of stored nitrogen in the rice grain, indicating that the strain DCY84 T enhanced plant nitrogen utilization with less nitrogen fertilizer application.…”

The effect of active forms of silicon on the biomass of agricultural crops during their growth period on technogenically altered soils of the Nikopol Manganese Ore Basin

“…Гістохімічні дослідження рису показали, що корені накопичують Si та розподіляють його рівномірно (Abe and Wakatsuki, 2011;Choi et al, 2016;Horgan et al, 2016), хоча вважалось, що він міститься переважно в епідермальних тканинах. У транспіраційних органах (листках) кремній концентрується в епідермальних тканинах, утворює подвійний кутикулярний кремнієвий шар, який захищає рослини від надмірної транспірації та грибкових патогенів.…”

“…Зміна біомаси гречки на дерново-літогенних ґрунтах на сіро-зелених глинах із додаванням кремнієвих добрив: 0,1%, 0,2%, 0,3% та 0,4% розчини SiO 2 У нашому вегетаційному експерименті біомаса рослин збільшувалась від 1,27 до 3,50 раза відносно контролю. У працях Choi et al (2016) обробка насіння рису SiO 2 збільшила удвічі свіжу біомасу, а сухув 1,7 раза відносно рослин, які попередньо не обробляли. Збільшився вміст білка в зерні з 6,0% до 7,2%.…”