The Effects of Riluzole on Neurological, Brain Biochemical, and Histological Changes in Early and Late Term of Sepsis in Rats

Toklu, Hale Z.; Uysal, Meral Keyer; Kabasakal, Levent; Şirvancı, Serap; Ercan, Feríha; Kaya, Mehmet

doi:10.1016/j.jss.2008.03.013

Cited by 75 publications

(33 citation statements)

References 47 publications

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“…It is a potentially life threatening condition complicated with important biochemical and histologic changes occurring especially within six hours [3]. The understanding of underlying mechanisms of sepsis has been improving [15].…”

Section: Discussionmentioning

confidence: 99%

“…During sepsis, brain is one of the first organs affected and up to 71% of patients demonstrate encephalopathy [1,2]. Sepsis associated encephalopathy (SAE) is the most common encephalopathy encountered among intensive care unit (ICU) patients and has been usually associated with poor prognosis [3,4]. SAE may cause serious cognitive dysfunctions, impaired consciousness, and delirium [5].…”

Section: Introductionmentioning

confidence: 99%

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Effects of Ecballium elaterium on brain in a rat model of sepsis-associated encephalopathy

Arslan

Ekinci

Arıcı

et al. 2017

Libyan Journal of Medicine

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Despite recent advances in antibiotic therapy, sepsis remains a major clinical challenge in intensive care units. Here we examined the anti-inflammatory and antioxidant effects of Ecballium elaterium (EE) on brain, and explored its therapeutic potential in an animal model of sepsis-associated encephalopathy (SAE) [induced by cecal ligation and puncture (CLP)].Thirty rats were divided into three groups of 10 each: control, sepsis, and treatment. Rats were subjected to CLP except for the control group, which underwent laparatomy only. The treatment group received 2.5 mg/kg EE while the sepsis group was administered by saline. Twenty-four hours after laparotomy, animals were sacrificied and the brains were removed. Brain homogenates were prepared to assess interleukin 1beta (IL-1β), interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-α), total antioxidant capacity (TAC), and total oxidant status (TOS). Brain tissue sections were stained by hematoxylin and eosin (H&E) to semi-quantitatively examine the histopathologic changes such as neuron degeneration, pericellular/perivascular edema and inflammatory cell infiltration in the cerebral cortex.We found a statistically significant reduction in brain tissue homogenate levels of TNF-α 59.5 ± 8.4/50.2 ± 6.2 (p = 0.007) and TOS 99.3 ± 16.9/82.3 ± 7.8 (p = 0.01) in rats treated with EE; although interleukin 6 levels were increased in the treatment group compared to the sepsis group, this was not statistically significant. Neuronal damage (p = 0.00), pericellular/perivascular edema and inflammatory cell infiltration (p = 0.001) were also significantly lower in the treatment group compared to those in the sepsis group.These data suggest that Ecballium elaterium contains some components that exert protective effects against SAE in part by attenuating accumulation of proinflammatory cytokines, which may be important contributors to its anti-inflammatory effects during sepsis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of Ecballium elaterium on brain in a rat model of sepsis-associated encephalopathy

Arslan

Ekinci

Arıcı

et al. 2017

Libyan Journal of Medicine

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“…The neurological examination scores were conducted according to Bederson's modified neurological examination test (33). A twenty-point neuroscore was used to assess motor and behavioral deficits.…”

Section: Neurological Examinationmentioning

confidence: 99%

“…Evans blue assay for the evaluation of blood brain barrier permeability To evaluate the blood brain barrier (BBB) integrity, Evans blue dye (EB) was used as a marker of albumin extravasation (33). Briefly, EB (2 % in saline, 4 ml/kg) was injected via the jugular vein at the 48 th h of the SAH induction and it was allowed to remain in circulation for 30 min.…”

Section: Neurological Examinationmentioning

confidence: 99%

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The protective effect of Nigella sativa oil in the brain of the biliary obstructed rats

Toklu¹

2013

mpj

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Oxidative stress is one of the important mechanisms of jaundice induced encephalopathy. The aim of this study was to examine the possible protective effect of Nigella sativa (NS) seed oil against the oxidative stress of brain tissue induced by experimental obstructive jaundice in rats.BiliarY obstruction was performed in male Wistar albino rats by bile duct ligation and scission (BDL). Intragastric NS oil (1 mg/kg p.o.) or saline was administered for 28 days. At the end of the experiment, in the half of the rats the blood brain barrier (BBB) permeability was evaluated by Evans blue (EB) extravasation. Other rats were decapitated and brain tissue samples were obtained for the measurement of malondialdehyde (MDA) and glutathione (GSH) levels, myeloperoxidase (MPO) and Na + ,K + -ATPase activities.ChronIC biliary obstruction caused a significant increase in the BBB permeability which was verified by EB extravasation while this effect was attenuated by NS oil treatment. On the other hand, BDL-induced decrease in brain GSH level and Na + ,K + -ATPase activity were elevated back to control level in NS oil-treated BDL group. Increase in tissue MDA level, and MPO activity due to BDL were also attenuated by NS oil treatment.Our results suggest that NS oil treatment protects the brain from oxidative damage following bile duct ligation in rats. This effect possibly involves the inhibition of neutrophil infiltration and lipid peroxidation and the restoration of antioxidant status in the tissue. Accordingly, supplementing cirrhotic patients with adjuvant therapy of NS oil may have some benefit against hepatic encephalopathy.

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Sepsis-associated Encephalopathy

Siami¹,

Polito²,

Sharshar³

2009

Intensive Care Medicine

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The Effects of Riluzole on Neurological, Brain Biochemical, and Histological Changes in Early and Late Term of Sepsis in Rats

Cited by 75 publications

References 47 publications

Effects of Ecballium elaterium on brain in a rat model of sepsis-associated encephalopathy

Effects of Ecballium elaterium on brain in a rat model of sepsis-associated encephalopathy

The protective effect of Nigella sativa oil in the brain of the biliary obstructed rats

Sepsis-associated Encephalopathy

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