The effects of roscovitine on cumulus cell apoptosis and the developmental competence of domestic cat oocytes

Sananmuang, Thanida; Techakumphu, Mongkol; Tharasanit, Theerawat

doi:10.1016/j.theriogenology.2009.08.013

Cited by 27 publications

(32 citation statements)

References 34 publications

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“…), pig (Romar and Funahashi ) and cat (Sananmuang et al. ). The effective concentration of roscovitine, the time of exposition capable to inhibit the meiotic resumption and its effects on oocyte ability to support fertilization and pre‐implantation embryo development are, however, variable among authors and studied species.…”

Section: Introductionmentioning

confidence: 99%

In vitro Developmental Competence of Adult Sheep Oocytes Treated with Roscovitine

Crocomo

Ariu

Bogliolo

et al. 2016

Reprod Domestic Animals

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The efficiency of in vitro sheep embryo production is still low compared to that observed in vivo and in other species. In this context, meiotic inhibition strategies emerged as a promising alternative to improve this biotechnology. So, this study aimed to evaluate, for the first time, the effects of roscovitine on in vitro maturation of sheep oocytes and their subsequent embryo development. For this, cumulus-oocyte complexes (COCs) were cultured for 6 h in the presence (Rosco) or absence (Control) of 75 μm roscovitine and, subsequently, in vitro matured (IVM) for 18 h with gonadotropins. At 0 (Immature), 6 and 24 h of culture, the nuclear status of oocytes was evaluated by Hoechst staining. Embryo cleavage and blastocyst formation were recorded 30 h after in vitro fertilization and on day 7 of culture, respectively. Blastocyst quality was evaluated by differential staining. At 6 h, the GV rate in the Rosco treatment (93.8%) was similar to that observed in the Immature oocytes (94.9%) and significantly higher compared to Control (41.3%). After IVM for 18 h, a high and similar proportion of oocytes from Rosco (93.6%) and Control (88.4%) reached the MII stage. In both treatments, approximately 70% of oocytes cleaved and 50% of them developed up to blastocyst. The mean percentage of blastocyst cells, embryoblast, trophoblast and pyknosis did also not differ between Control and Rosco. In conclusion, roscovitine, at the studied experimental conditions, was efficient to reversibly inhibit the meiosis of adult sheep oocytes without detrimental effect on development and quality of the in vitro produced embryos.

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Section: Introductionmentioning

confidence: 99%

In vitro Developmental Competence of Adult Sheep Oocytes Treated with Roscovitine

Crocomo

Ariu

Bogliolo

et al. 2016

Reprod Domestic Animals

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“…As we mentioned, the two concentrations studied were (1) the concentration normally used for IVM in IVF clinics (low) and (2) a physiological dose (high) equivalent for the reported surge in cats. In literature, however, gonadotropins of porcine or equine origin, plus higher concentrations, were more commonly used for feline studies [7,19,[24][25][26][27][28][29][30][31]. This was presumably due to experience from in vivo stimulation protocols, where porcine FSH and eCG were used to initiate folliculogenesis in felines (for more information please read review: [36]).…”

Section: Discussionmentioning

confidence: 99%

“…Regarding the domestic cat, efforts to date have focused on oocytes [7,[24][25][26][27][28][29][30] or preantral follicle cultures [19,31]. Therefore, studies were designed to characterize and evaluate the response of individual intact antral follicles from adult female domestic cats to a stimulus of recombinant human LH in vitro by assessing cumulus-oocyte expansion (C-OE) and steroid production (E2 and P4).…”

Section: Introductionmentioning

confidence: 99%

Felis catus ovary as a model to study follicle biology in vitro

Rojo

Linari

Musse

et al. 2015

J Assist Reprod Genet

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Purpose The current study was designed to evaluate the response of individual intact antral follicles from adult female domestic cats to a luteinizing hormone (LH) stimulus in vitro by assessing cumulus-oocyte expansion (C-OE) and steroid production. Methods C-OE and steroid levels (estradiol [E2] and progesterone [P4]) obtained from individual antral feline follicles (n=366 follicles; n=56 cats) were analyzed after 12 or 24 h of culture in the presence or absence of LH (low [3.4 ng/ml] or high [100 ng/ml]). Results At the end of the culture, the highest percentage of expanded cumulus-oocyte complexes (COCs) was observed in the LH groups at 12 or 24 h in comparison to their controls (p<0.001). There was a significant increase in expanded COCs when comparing LH concentrations (high vs. low) at 12 or 24 h. Higher levels of both E2 and P4 were observed in the media from antral follicles after 12 and 24 h of culture in the presence of LH (both concentration, p<0.05). There was no association between hormone levels and follicle diameter; high variability was observed in the steroid levels produced by antral follicles within all treatment groups.Conclusions These data indicate, for the first time, that feline antral follicles (0.5-2 mm) from different stages of the natural estrous cycle can be cultured and will respond to an LH stimulus, based on an increase in steroid levels as well as C-OE after 12 or 24 h in culture.

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“…In vitro oocyte maturation (IVM) and fertilization (IVF) were essentially performed as previously described [30]. Groups of 20–30 COCs were cultured for 24 h in 500 μl of IVM medium (NaHCO 3 -M199 supplemented with 1.0 mM sodium pyruvate, 2.0 mM L-glutamine, 100 IU/ml penicillin, 100 µg/ml streptomycin, 4 mg/ml bovine serum albumin (BSA) and 25 ng/ml epidermal growth factor (EGF)) containing with 0.05 IU/ml recombinant human follicle stimulating hormone (rhFSH, Organon, The Netherlands).…”

Section: Methodsmentioning

confidence: 99%

<i>In Vitro</i> Culture of Feline Embryos Increases Stress-induced Heat Shock Protein 70 and Apoptotic Related Genes

Sananmuang

Phutikanit

Nguyen

et al. 2013

Journal of Reproduction and Development

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Developmental competence and quality of in vitro produced embryos has been demonstrated to be lower than in vivo derived embryos. This study aimed specifically to determine the effects of in vitro culture of feline embryos using various culture densities on developmental competence and expression of stress- and apoptotic-related genes in terms of heat shock protein 70 (HSP70) and apoptotic-related (BAX and BCL-2) gene expressions. In experiment 1, we characterized the inducible form of a feline-specific HSP70 mRNA sequence, as it has not been previously reported. The primers for feline HSP70 mRNA were synthesized and tested on heat-treated cat fibroblasts. In experiment 2, feline embryos were cultured at different culture densities (embryo:culture volume; 1:1.25, 1:5 and 1:20). The developmental competence was determined along with HSP70, BAX and BCL-2 transcript abundances using quantitative RT-PCR. In vivo derived embryos were used as a control group. A partial cat HSP70 mRNA sequence (190 bp) was characterized and exhibited high nucleotide identity (93 to 96%) with other species. Cleaved embryos cultured at high density (1:1.25) developed to blastocysts at a lower rate than those generated from lower densities. Irrespective of the culture densities used, in vitro cultured blastocysts showed increased levels of HSP70 and BAX transcripts compared with in vivo counterparts. Blastocysts derived from the highest culture density (1:1.25) showed higher levels of upregulation of HSP70 and BAX transcripts than those cultured at lower culture densities (1:5 and 1:20). In conclusion, increased levels of pro-apoptotic (BAX) and stress-response (HSP70) transcripts correlated with developmental incompetence of embryos cultured at high embryonic density, indicating that stress accumulated during in vitro embryo culture affected the fate for embryo development and quality.

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The effects of roscovitine on cumulus cell apoptosis and the developmental competence of domestic cat oocytes

Cited by 27 publications

References 34 publications

In vitro Developmental Competence of Adult Sheep Oocytes Treated with Roscovitine

In vitro Developmental Competence of Adult Sheep Oocytes Treated with Roscovitine

Felis catus ovary as a model to study follicle biology in vitro

<i>In Vitro</i> Culture of Feline Embryos Increases Stress-induced Heat Shock Protein 70 and Apoptotic Related Genes

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