The effects of rosmarinic acid on oxidative stress parameters and inflammatory cytokines in lipopolysaccharide-induced peripheral blood mononuclear cells

Sadeghi, Asie; Bastin, Ali Reza; Ghahremani, Hossein; Doustimotlagh, Amir Hossein

doi:10.1007/s11033-020-05447-x

Cited by 42 publications

(21 citation statements)

References 50 publications

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“…The ability of thiols to oxidize 5,5-dithiobis-2-nitrobenzoic (DTNB) was evaluated by measuring the absorbance at 412 nm [ 25 ]. 25 μ l of the sample (lysed treated HepG2 cells) was added to Tris buffer (150 μ l).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the Antioxidant and Anticancer Activities of Hydroalcoholic Extracts of Thymus daenensis Čelak and Stachys pilifera Benth

Barmoudeh

Ardakani

Doustimotlagh

et al. 2022

Journal of Toxicology

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Herein, the effects of hydroalcoholic extracts of Thymus daenensis Celak (TDC) and Stachys pilifera Benth (SPB) plants on HepG2 cell line were investigated by using different analyses. Cytotoxicity and apoptosis of extracts were investigated by MTT method, AnnV/PI apoptosis assay, and their antioxidant capacity was evaluated by total thiol and glutathione peroxidase (GPX) assay. The results revealed that the SBP extract was more cytotoxic compared with the TDC extract and increased over time (128.49 µg/mL vs 107.11 µg/mL IC50 values for 24 and 72 h, respectively). Although, AnnV/PI apoptosis assay showed apoptosis induction for both extracts, but the caspase-3 activity assay revealed that TDC extract significantly increased caspase-3 activity compared with the control and SPB extract. Increasing the activity of GPX by SPB extract revealed that it has high antioxidant capacity. In conclusion, the TDC and SPB with high antioxidant capacity have high cytotoxicity against HepG2 cancer cells and have high capability as a medicinal plant.

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Section: Methodsmentioning

confidence: 99%

Evaluation of the Antioxidant and Anticancer Activities of Hydroalcoholic Extracts of Thymus daenensis Čelak and Stachys pilifera Benth

Barmoudeh

Ardakani

Doustimotlagh

et al. 2022

Journal of Toxicology

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“…Serum levels of urea and creatinine were evaluated using commercial kits (Pars Azmoon, Iran). The T-SH content was determined using the spectrophotometric method ( 14 ). Benzie and Strain procedure was applied to measure FRAP ( 14 ).…”

Section: Methodsmentioning

confidence: 99%

“…The T-SH content was determined using the spectrophotometric method ( 14 ). Benzie and Strain procedure was applied to measure FRAP ( 14 ). Tissue NO metabolite (nitrite) was measured as indices of NO production according to the Griess reaction ( 15 ).…”

Section: Methodsmentioning

confidence: 99%

Protective effects of hydroalcoholic extract of Stachys pilifera on paracetamol-induced nephrotoxicity in female rats

Rabani

Azarmehr

Moslemi

et al. 2021

Research in Pharmaceutical Sciences

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Background and purpose: Stachys pilifera is used in traditional medicine due to its antioxidant, anti-inflammatory, and antimicrobial effects. The goal of this study was to examine the renoprotective activity of the hydroalcoholic extract of aerial parts of S. pilifera on paracetamol (PCM)-induced nephrotoxicity. Experimental approach: The Wistar female rats were randomly divided into four groups including control, PCM, S. pilifera hydroalcoholic extract (SPE), and PCM + SPE. The animals received SPE (500 mg/kg) for one week and PCM (3 g/kg) on the 6 th day orally. Kidney function tests and oxidant/antioxidant markers were determined in serum and tissue homogenate, respectively. Protein and mRNA levels of TNF-α, as well as hematoxylin and eosin staining, were assessed in the kidney tissue. Findings/Results: Treatment with SPE in the PCM group significantly decreased blood urea nitrogen and creatinine against the merely PCM rats ( P < 0.05). The amount of nitric oxide metabolite and superoxide dismutase activity in the group receiving SPE showed a significant increase compared to PCM rats ( P < 0.05). A significant difference in TNF-α levels between the groups was not observed. Histological changes were improved in the rats treated with SPE. Conclusion and implications: Totally, our findings showed that SPE can inhibit PCM nephrotoxicity by enhancing kidney function markers, antioxidant status, and histological changes. Though, more researches are required to estimate the possible mechanism of SPE.

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“…Among essential oils, oregano essential oil (ORE) is used in the pig industry for its beneficial effects, ranging from improved performance to increased pork quality (31,32). It has been shown that oregano has anti-inflammatory and antimicrobial effects (33)(34)(35), by modulating the cytokine levels and immunity-related transcription factors (36,37). In addition, oregano stimulates digestion with effects on enterocytes, by accelerating their renewal rate, improving their capacity for nutrient absorption, and reducing pathogen contamination (38).…”

Section: Introductionmentioning

confidence: 99%

Differential Effects of Dietary Oregano Essential Oil on the Inflammation Related Gene Expression in Peripheral Blood Mononuclear Cells From Outdoor and Indoor Reared Pigs

et al. 2021

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Intensive farming systems represent a stressful environment for pigs and negatively influence neuroendocrine functions, behavior, and performance. Outdoor farming is an alternative option, which is thought to imply several beneficial effects for the animal. Dietary essential oils are known to be an innovative strategy to improve pig health and performance, and oregano essential oil (ORE) possesses beneficial effects due to its antimicrobial, anti-fungal, and antioxidant properties. We tested the effect of dietary ORE on peripheral blood mononuclear cells (PBMCs) in 36 growing pigs, either reared under indoor or outdoor conditions. Quantitative real-time PCR (RT-qPCR) assay was used to evaluate the effect of diet (control vs. ORE) and the time of sampling (T1−120 days vs. T2−190 days) on the expression of inflammatory and immune-related genes (TNF, IL1β, IL8, IL18, IL10, IL1RN, STAT3, HSP90, ICAM-1, and NFKB1). Under outdoor condition, the majority of transcripts were upregulated (p < 0.05), assuming a general inflammatory status (TNF, HSP90, NFKB1, IL1β, and STAT3). However, an interaction between diet and the farming system was observed: HSP90, NFKB1, and STAT3 were downregulated (p < 0.05) in the outdoor reared pigs when fed the ORE diet. Our study showed that bioactive compounds of ORE exert their activity, especially when the animals are exposed to stressful stimuli. Dietary ORE can be an acceptable strategy to help pigs tolerate the stress related to the harsh, outdoor, rearing conditions.

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The effects of rosmarinic acid on oxidative stress parameters and inflammatory cytokines in lipopolysaccharide-induced peripheral blood mononuclear cells

Cited by 42 publications

References 50 publications

Evaluation of the Antioxidant and Anticancer Activities of Hydroalcoholic Extracts of Thymus daenensis Čelak and Stachys pilifera Benth

Evaluation of the Antioxidant and Anticancer Activities of Hydroalcoholic Extracts of Thymus daenensis Čelak and Stachys pilifera Benth

Protective effects of hydroalcoholic extract of Stachys pilifera on paracetamol-induced nephrotoxicity in female rats

Differential Effects of Dietary Oregano Essential Oil on the Inflammation Related Gene Expression in Peripheral Blood Mononuclear Cells From Outdoor and Indoor Reared Pigs

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