The Effects of Rotating Magnetic Field on Growth Rate, Cell Metabolic Activity and Biofilm Formation by Staphylococcus Aureus and Escherichia Coli

Abstract

This work presents results of the study which concerns the influence of the rotating magnetic field (RMF) on the growth rate, cell metabolic activity and ability to form biofilms by E. coli and S. aureus. Liquid cultures of the bacteria were exposed to the RMF (RMF frequency f = 1-50 Hz, RMF magnetic induction B = 22-34 mT, time of exposure t = 60 min, temperature of incubation 37 °C). The present study indicate the exposition to the RMF, as compared to the unexposed controls causing an increase in the growth dynamics, cell metabolic activities and percentage of biofilm-forming bacteria, in both S. aureus and E. coli cultures. It was also found that the stimulating effects of the RMF exposition enhanced with its increasing frequencies and magnetic inductions.

The Effect of Rotating Magnetic Field on Enterotoxin Genes Expression in Staphylococcus Aureus Strains

“…The present work is a continuation of our previous study [20] in which we investigated whether the RMF, depending on magnetic induction, frequency and time of exposure, can influence on the growth dynamics, cell metabolic activity and other biochemical processes in various microorganism, including S. aureus. In the current study, we investigated the effect of the RMF exposure on pathogenic potential of S. aureus strains expressed by the changes in activity of genes encoding staphylococcal enterotoxins.…”

“…The exposition of bacteria to the RMF was carried out using self-designed set-up, described in our previous work [20] and adopted for purposes of this research. This set-up is schematically presented in Fig.…”

Biochemical and cellular properties of Gluconacetobacter xylinus cultures exposed to different modes of rotating magnetic field

“…There are numerous reports showing that MFs may have infl uence on microorganisms and thus change their biotechnological potential [16][17][18] . The research concerning the use of MFs in the biotechnological engineering conducted to date have concerned mostly static magnetic fi elds (SMFs), whereas the biotechnologically promising idea to use rotating magnetic fi elds (RMFs) is developed just relatively recently [19][20][21] . In previous studies we showed that the RMF can be used to increase (over 20% compared to the bacteria non-exposed to RMF) the growth and cell metabolic activity of such microorganisms as Staphylococcus aureus, Escherichia coli, Serratia marcescens, Streptococcus mutans, Cronobacter sakazakii, Klebsiella oxytoca and Staphylococcus xylosus) [20][21] .…”

“…The research concerning the use of MFs in the biotechnological engineering conducted to date have concerned mostly static magnetic fi elds (SMFs), whereas the biotechnologically promising idea to use rotating magnetic fi elds (RMFs) is developed just relatively recently [19][20][21] . In previous studies we showed that the RMF can be used to increase (over 20% compared to the bacteria non-exposed to RMF) the growth and cell metabolic activity of such microorganisms as Staphylococcus aureus, Escherichia coli, Serratia marcescens, Streptococcus mutans, Cronobacter sakazakii, Klebsiella oxytoca and Staphylococcus xylosus) [20][21] . Similarly, the current study also showed that, depending on its mode, RMF exposure increased the growth of G. xylinus by as much as 43% as compared to the control unexposed cultures.…”

Pengaruh Pemaparan Medan Magnet Pada Media Mandels Yang Dimodifikasi Terhadap Pertumbuhan Dan Aktivitas Enzim Selulase Bacillus Sp.

“…Matwijczuk et al (2011), juga menyatakan bahwa pemaparan medan magnet baik pada biji bunga matahari langsung maupun pada air untuk menyiram kecambah bunga matahari dapat meningkatkan kecepatan perkecambahan. Fijalkowski, et al (2013), menyatakan bahwa pemaparan medan magnet pada bakteri Escherichia Coli dan Staphylococcus Aureus dengan frekuensi 1 -50 Hz selama 60 menit pada suhu inkubasi 37 °C dapat meningkatkan pertumbuhan, aktivitas metabolisme dan kemampuan pembentukan bio-film. Hasil yang berbeda ditunjukkan dari hasil penelitian Fojt, et al (2009) yang menyatakan bahwa pemaparan medan magnet yang semakin lama, mengakibatkan penurunan pertumbuhan bakteri E. coli dan P. denitrificans.…”