The effects of salicylic acid pretreatment on the antioxidant enzymatic system of chickpea seedlings under salt stress

Soare, Marin; Soare, Rodica; Babeanu, Cristina; Bonea, Dorina

doi:10.1016/j.jbiotec.2015.06.363

“…Positive correlations have been observed when DPPH radical scavenging activities were compared with total phenolic compounds content and total flavonoids content, thus indicating that these compounds are responsible for the antioxidant activity. This positive correlation was also reported for other plant species [9,10].…”

Section: Resultssupporting

confidence: 86%

“…Total phenolic compounds (TPC) content was determined colorimetric by using the Folin-Ciocalteu method [9] based on the oxidation of phenolic groups with phosphomolybdic and phosphotungstic acids. 2 mL Folin-Ciocalteu's phenol reagent (1:10) and 1.5 mL 7.5% w/v Na2CO3 were added to 0.5 mL sample extract.…”

Section: Analysis Methodsmentioning

confidence: 99%

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Total Phenolic, Total Flavonoids Content and Antioxidant Activity in Fruits of Four Plum (Prunus Domestica L.) Cultivars

Babeanu

¹

,

Ciobanu

²

2021

AUC Chem

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Plums are among the most consumed fruits in Romania, being a good source of health promoting compounds. The objective of this paper is to evaluate in comparison the total phenolic content, the flavonoids and anthocyanins content and antioxidant activity in fruit of four plum cultivars. The total phenolic, flavonoids and anthocyanin contents were determined by colorimetric methods and the antioxidant activity was evaluated by DPPH radical scavenging assay. The results show that studied chemical indices vary depending on the analyzed cultivar. The obtained results show that all the studied cultivars show a high phytochemicals content and significant antioxidant activity and recommend their use as sources of phenolic compounds and natural antioxidants.

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“…Total phenolic compounds (TPC) content was determined colorimetric by using the Folin-Ciocalteu method [9] based on the oxidation of phenolic groups with phosphomolybdic and phosphotungstic acids. 2 mL Folin-Ciocalteu's phenol reagent (1:10) and 1.5 mL 7.5% w/v Na2CO3 were added to 0.5 mL sample extract.…”

Section: Analysis Methodsmentioning

confidence: 99%

Total Phenolic, Total Flavonoids Content and Antioxidant Activity in Fruits of Four Plum (Prunus Domestica L.) Cultivars

Babeanu

¹

,

Ciobanu

²

2021

AUC Chem

Self Cite

0

View full text Add to dashboard Cite

Plums are among the most consumed fruits in Romania, being a good source of health promoting compounds. The objective of this paper is to evaluate in comparison the total phenolic content, the flavonoids and anthocyanins content and antioxidant activity in fruit of four plum cultivars. The total phenolic, flavonoids and anthocyanin contents were determined by colorimetric methods and the antioxidant activity was evaluated by DPPH radical scavenging assay. The results show that studied chemical indices vary depending on the analyzed cultivar. The obtained results show that all the studied cultivars show a high phytochemicals content and significant antioxidant activity and recommend their use as sources of phenolic compounds and natural antioxidants.

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“…Moreover, the measurement of uncertainty in the TPC analysis was not reported. Analytical Romanian studies have been focused only on estimation of total phenolic content (TPC) from plant extracts by UV-Vis spectrometric method [33][34][35][36][37][38]. Thus, they could not meet the current technical requirement of ISO:17025-2017 of accurate estimation.…”

Section: Introductionmentioning

confidence: 99%

Determination of Total Phenolic Content From Plant Extracts Used in Cosmetic Purpose

Dobrinaș

¹

,

Soceanu

²

2021

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Spectrometric analysis belongs to a group of measurement methods used for their simplicity and large selectivity to solve various problems of analytes. The total phenolic content of different plant extracts used for cosmetic purpose was determined by a slightly modified version of traditionally Folin-Ciocalteau method. The objective of this work was the validation of UV-Vis spectrometric method, investigation of uncertainty sources when measuring gallic acid concentration and evaluation of the calibration equation effect on measurement uncertainty of UV– Vis spectrophotometer. Validation was performed by studying analytical curve linearity (R2=0.9995) and range (37.5 – 225 mg L-1), estimated limit of detection (LOD, 0.11mg L-1) and limit of quantification (LOQ, 0.37 mg L-1), precision (%RSD, 0.14 – 1.34), recoveries (83-110%) and stability (%RSD, 0.8 – 2.83). To obtain more representative values for precision, recoveries and stability simultaneous replicates at different times, on different matrices including plant (marigold, chamomile and lavender) were performed during the study period. The validated method was successfully applied to determine TPC in marigold extracts. For chamomile and lavender extracts, the spectrometric method presented only acceptable precision, among all the performance parameters studied. The sources of the gallic acid concentration measurement uncertainty include purity, volume of flasks, mass and the calibration equation. The results indicated that the uncertainty components from purity were the smallest. An important source of the uncertainty was the mass.The volumes of the volumetric flasks had only modest effect on the uncertainty. The contribution of calibration equation is the greatest from all sources.

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The effects of salicylic acid pretreatment on the antioxidant enzymatic system of chickpea seedlings under salt stress

Cited by 3 publications

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Total Phenolic, Total Flavonoids Content and Antioxidant Activity in Fruits of Four Plum (Prunus Domestica L.) Cultivars

Total Phenolic, Total Flavonoids Content and Antioxidant Activity in Fruits of Four Plum (Prunus Domestica L.) Cultivars

Total Phenolic, Total Flavonoids Content and Antioxidant Activity in Fruits of Four Plum (Prunus Domestica L.) Cultivars

Determination of Total Phenolic Content From Plant Extracts Used in Cosmetic Purpose

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