The effects of short-term hypergravity on Caenorhabditis elegans

Saldanha, Jenifer N.; Pandey, Santosh; Powell-Coffman, Jo Anne

doi:10.1016/j.lssr.2016.06.003

Cited by 9 publications

(6 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, the nematode Caenorhabditis elegans ( C. elegans ) represents a primary model for space life sciences owing to its small size, short life span, ease of culture, low expense ( Brenner, 1974 ), and a completely defined genome ( C. elegans Sequencing Consortium, 1998 ) with strong evolutionary conservation in humans ( Lai et al., 2000 ). Previous work demonstrates C. elegans are capable of successful reproductive cycles in both microgravity ( Oczypok et al., 2012 ) and hypergravity ( Qiao et al., 2013 ; Saldanha et al., 2016 ) and survive even when exposed to hypergravitational forces upward of 400,000 × g ( de Souza and Pereira, 2018 ). Moreover, in microgravity C. elegans display molecular signatures (e.g., impaired insulin and cell adhesion signaling) and physiological features (e.g., reduced movement capacity) that closely mirror those observed in humans ( Higashibata et al., 2006 , 2016 ; Nichols et al., 2006 ; Selch et al., 2008 ).…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptomics Identifies Neuronal and Metabolic Adaptations to Hypergravity and Microgravity in Caenorhabditis elegans

et al. 2020

View full text Add to dashboard Cite

Deep space exploration is firmly within reach, but health decline during extended spaceflight remains a key challenge. In this study, we performed comparative transcriptomic analysis of Caenorhabditis elegans responses to varying degrees of hypergravity and to two spaceflight experiments (ICE-FIRST and CERISE). We found that progressive hypergravitational load concomitantly increases the extent of differential gene regulation and that subtle changes in 1,000 genes are reproducibly observed during spaceflight-induced microgravity. Consequently, we deduce those genes that are concordantly regulated by altered gravity per se or that display inverted expression profiles during hypergravity versus microgravity. Through doing so, we identify several candidate targets with terrestrial roles in neuronal function and/or cellular metabolism, which are linked to regulation by daf-16/FOXO signaling. These data offer a strong foundation from which to expedite mechanistic understanding of spaceflight-induced maladaptation in higher organisms and, ultimately, promote future targeted therapeutic development.

show abstract

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptomics Identifies Neuronal and Metabolic Adaptations to Hypergravity and Microgravity in Caenorhabditis elegans

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Meanwhile, it is interesting to find that the number of luminous sites and their fluorescence intensity under microgravity increased faster than in normal conditions, implying the increased content of gene‐encoded fluorescence protein. It has been reported previously that microgravity may affect the gene expression associated with muscle‐related biomolecules, such as myosin and para‐muscle proteins that are the main component of myofilaments . It seems that the observed fluorescence enhancement in the nematode may be correlated to the expression of protein.…”

Section: Resultsmentioning

confidence: 88%

A novel microfluidic capture and monitoring method for assessing physiological damage of C. elegans under microgravity

et al. 2019

View full text Add to dashboard Cite

Spatial microgravity is a significant factor affecting and causing physiological changes of organisms in space environment. On‐site assessment of the damage associated to microgravity is very important for future long‐term space exploration of mankind. In this paper, a new microfluidic device for analyzing the damage of microgravity on Caenorhabditis elegans (C. elegans) has been developed. This device is mainly composed of a microfluidic chip, a dual imaging module, and an imaging acquisition and processing module, which are integrated into a compact system. The microfluidic chip is designed as a platform for monitoring C. elegans, which is captured in an imaging region through a suction structure in the microfluidic chip. A dual imaging module is designed to obtain the images of bright field and fluorescence of C. elegans. The behaviors of C. elegans are analyzed based on the dual‐mode imaging of bright field and fluorescence to assess the degree of damage due to microgravity. A comparative study using a commercial microscope is also conducted to demonstrate the unique advantage of the developed system under the simulated microgravity. The results show that the developed system can evaluate the damage of C. elegans under microgravity accurately and conveniently. Furthermore, this device has compact size and weight, easy operation, and low‐cost, which could be highly advantageous for on‐site evaluation of the damage to microorganisms under microgravity in a space station.

show abstract

“…Recent studies have also focused on the effects of hyper-gravity on the microbial proliferation except for survival. A study found that ultracentrifugation for a short time can induce soil microbial division to resist stress [41]. Similarly, a recent study revealed a variety of microorganisms, including Gram-negative E. coli and Gram-positive Lactobacillus delbrueckii.…”

Section: Discussionmentioning

confidence: 99%

Survival of soil microbial community exposed to hyper-gravity conditions

Xu¹,

Liu²,

Zhao³

et al. 2021

Preprint

View full text Add to dashboard Cite

Earth is the cradle of mankind, but it is impossible for human beings to live in the cradle forever. Sending soil microbial spores through space to foreign planets will be a likely initial process in planet colonization. Periods of hyper-gravity are likely to be a challenge for the candidate microorganisms during their interstellar transportation, raising questions about their survival rates and community-level responses. To address these questions, the impacts of hyper-gravity on soil microbial community composition and activity were tested by applying 1×g or 2500×g centrifugal force to soil for 6 days. The results indicated an increased diversity and absolute abundance of soil total bacterial community and a relatively stable active bacterial community under hyper-gravity condition. Besides, hyper-gravity had no observable effect on the relative abundance of soil microorganisms. These results suggest that soil microorganisms could survive during short periods of hyper-gravity. Our findings represent the first step towards a better understanding of the potential for survival of soil microbiomes during space travel and provide a basis for further interstellar soil research.

show abstract

The effects of short-term hypergravity on Caenorhabditis elegans

Cited by 9 publications

References 48 publications

Comparative Transcriptomics Identifies Neuronal and Metabolic Adaptations to Hypergravity and Microgravity in Caenorhabditis elegans

Comparative Transcriptomics Identifies Neuronal and Metabolic Adaptations to Hypergravity and Microgravity in Caenorhabditis elegans

A novel microfluidic capture and monitoring method for assessing physiological damage of C. elegans under microgravity

Survival of soil microbial community exposed to hyper-gravity conditions

Contact Info

Product

Resources

About