The Effects of Silencing PTX3 on the Proteome of Human Endothelial Cells

Banfi, Cristina; Brioschi, Maura; Vicentini, L.; Cattaneo, Maria Grazia

doi:10.3390/ijms232113487

Cited by 5 publications

(4 citation statements)

References 71 publications

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“…Interestingly, in a PTX3 deficient mice model, kidney injury is enhanced because P-selectin is reported to be downregulated by PTX3 with a consequent inhibitory effect on leukocyte recruitment in a post ischemic model of kidney injury [34]. Consistent with these data, the lack of PTX3 induced an increase in pro-inflammatory markers and a shift to the more oxidative profile of PTX3-deficient ECs supporting the idea of a protective role for PTX3 in endothelial homeostasis [35].…”

Section: Ptx3 and Tissue Remodelling In Ckdsupporting

confidence: 54%

Pentraxin 3 in Kidney Disease and Renal Replacement Therapies

Losappio¹,

Infante²,

Maiorano³

et al. 2023

Preprint

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Chronic kidney disease (CKD) is actually considered a public health priority according to the increasing number of patients affected by this condition: this casuistry is not only related to specific glomerular, tubular or autoimmune diseases or a consequence of acute kidney injury (AKI) episodes leading to organ failure, but it is tied to the progression of life expectancy and the impact of comorbidities such as cardiovascular disease, diabetes and cancer. CKD and its comorbidities promote low grade inflammatory status with an impact on patients’ clinical conditions, reducing their possibility for kidney transplantation or graft survival and their survival when receiving renal replacement therapies such as hemodialysis (HD) or peritoneal dialysis (PD). CKD is often referred to as an ageing accelerator: innate immune system dysregulation, in the uraemic proinflammatory milieu, is involved in this accelerated senescence phenomena and pentraxins, particularly Pentraxin-3 (PTX-3), are of particular interest in the development of kidney disease. A complete understanding of the mechanism of CKD progression, innate immune system involvement and a proper definition of PTX-3 role in kidney disease, could redefine the approach for diagnosis and a more centered patients’ management to slow down CKD progression over time and reduce its clinical and social impact.

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Section: Ptx3 and Tissue Remodelling In Ckdsupporting

confidence: 54%

Pentraxin 3 in Kidney Disease and Renal Replacement Therapies

Losappio¹,

Infante²,

Maiorano³

et al. 2023

Preprint

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“…Label-free mass spectrometry analysis, LC-MSE, was performed on a hybrid quadrupole-time of flight mass spectrometer (Synapt XS, Waters corporation, Milford, MA, USA) coupled with a UPLC Mclass system with 2D LC Technology and equipped with a nanosource (Waters Corporation, Milford USA) [ 37 ]. An orthogonal 2D reversed-phase (RP/RP) approach was used, with the sample (500 ng) loaded at pH 10 and separated into five fractions at pH 10 before analytical separation at pH 2.5 after dilution using a trap column.…”

Section: Methodsmentioning

confidence: 99%

Proteome Profiling of the Dura Mater in Patients with Moyamoya Angiopathy

Carrozzini

Pollaci

Gorla

et al. 2023

IJMS

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Moyamoya angiopathy (MMA) is an uncommon cerebrovascular disease characterized by a progressive steno-occlusive lesion of the internal carotid artery and the compensatory development of an unstable network of collateral vessels. These vascular hallmarks are responsible for recurrent ischemic/hemorrhagic strokes. Surgical treatment represents the preferred procedure for MMA patients, and indirect revascularization may induce a spontaneous angiogenesis between the brain surface and dura mater (DM), whose function remains rather unknown. A better understanding of MMA pathogenesis is expected from the molecular characterization of DM. We performed a comprehensive, label-free, quantitative mass spectrometry-based proteomic characterization of DM. The 30 most abundant identified proteins were located in the extracellular region or exosomes and were involved in extracellular matrix organization. Gene ontology analysis revealed that most proteins were involved in binding functions and hydrolase activity. Among the 30 most abundant proteins, Filamin A is particularly relevant because considering its well-known biochemical functions and molecular features, it could be a possible second hit gene with a potential role in MMA pathogenesis. The current explorative study could pave the way for further analyses aimed at better understanding such uncommon and disabling intracranial vasculopathy.

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“…After digestion, 2% trifluoroacetic acid (TFA) was added to hydrolyze RapiGest and inactivated trypsin. Tryptic peptides were used for label-free mass spectrometry analysis, LC-MS E , performed on a hybrid quadrupole-time of flight mass spectrometer (SYNAPT-XS, Waters Corporation, Milford, MA, USA) coupled with a UPLC Mclass system and equipped with a nano-source (Waters Corporation, Milford, MA, USA), as previously described [ 8 , 9 ]. Statistical analysis was performed by means of Progenesis QIP v 4.1 (Nonlinear Dynamics) using a Uniprot mouse protein sequence database (v2020).…”

Section: Methodsmentioning

confidence: 99%

Prenylcysteine Oxidase 1 Is a Key Regulator of Adipogenesis

et al. 2023

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The process of adipogenesis involves the differentiation of preadipocytes into mature adipocytes. Excessive adipogenesis promotes obesity, a condition that increasingly threatens global health and contributes to the rapid rise of obesity-related diseases. We have recently shown that prenylcysteine oxidase 1 (PCYOX1) is a regulator of atherosclerosis-disease mechanisms, which acts through mechanisms not exclusively related to its pro-oxidant activity. To address the role of PCYOX1 in the adipogenic process, we extended our previous observations confirming that Pcyox1−/−/Apoe−/− mice fed a high-fat diet for 8 or 12 weeks showed significantly lower body weight, when compared to Pcyox1+/+/Apoe−/− mice, due to an evident reduction in visceral adipose content. We herein assessed the role of PCYOX1 in adipogenesis. Here, we found that PCYOX1 is expressed in adipose tissue, and, independently from its pro-oxidant enzymatic activity, is critical for adipogenesis. Pcyox1 gene silencing completely prevented the differentiation of 3T3-L1 preadipocytes, by acting as an upstream regulator of several key players, such as FABP4, PPARγ, C/EBPα. Proteomic analysis, performed by quantitative label-free mass spectrometry, further strengthened the role of PCYOX1 in adipogenesis by expanding the list of its downstream targets. Finally, the absence of Pcyox1 reduces the inflammatory markers in adipose tissue. These findings render PCYOX1 a novel adipogenic factor with possible pathophysiological or therapeutic potential.

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The Effects of Silencing PTX3 on the Proteome of Human Endothelial Cells

Cited by 5 publications

References 71 publications

Pentraxin 3 in Kidney Disease and Renal Replacement Therapies

Pentraxin 3 in Kidney Disease and Renal Replacement Therapies

Proteome Profiling of the Dura Mater in Patients with Moyamoya Angiopathy

Prenylcysteine Oxidase 1 Is a Key Regulator of Adipogenesis

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