The effects of silymarin on the attachment and viability of primary cultures of rat hepatocytes

Sainz-Pardo, L.A.; Anundi, Iréne; Míguez, María Prado; Lindros, Kai O.

doi:10.1016/0887-2333(94)90020-5

Cited by 6 publications

(2 citation statements)

References 5 publications

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“…Various studies indicated that silibinin significantly increases glutathione levels, which serve as a free radical scavenger (Das and Vasudevan, 2006). Silibinin is relatively hydrophobic and its penetration into the cell is limited (Sainz-Pardo et al, 1994) and membrane structures are postulated to be one of the cellular targets for silibinin as it interacts with the surface of lipid bilayer (Wesolowska et al, 2007), its effect on erythrocytes may be explained by their incorporation into these lipid bilayers of the cell membrane leading to reducing hemolysis. Silibinin on the other hand, is able to increase the activity of both superoxide dismutase and glutathione peroxidase, which may explain the protective effect of the drug against free radicals and also stabilizing the effect on the red cell membrane (Altorjay et al, 1992).…”

Section: Controlmentioning

confidence: 98%

Free radical scavenging activity of silibinin in nitrite-induced hemoglobin oxidation and membrane fragility models

Marouf

Zalzala

Al-Khalifa

et al. 2011

Saudi Pharmaceutical Journal

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Free radical formation in heme proteins is recognized as a factor in mediating the toxicity of many drugs. Xenobiotics and drug therapy-related toxicity, due to oxidative modification of hemoglobin (Hb), has been attributed in part to the uncontrolled oxidative reactions. A variety of antioxidant strategies to ameliorate potential oxidative damage in vivo have been suggested. The present study was designed to evaluate the dose-response relationship of the free radical scavenging properties of silibinin dihemisuccinate (SDH) in nitrite-induced Hb oxidation in vitro and in vivo. Different concentrations of SDH were added, before and after different intervals of inducing Hb oxidation in erythrocytes lysate, and formation of methemoglobin (MetHb) was monitored spectrophotometrically; the same approach was utilized to evaluate the effect of the same doses of SDH on the integrity of erythrocytes after induction of hemolysis. Moreover, the most effective dose of SDH was administered in rats before challenge with toxic dose of sodium nitrite, and MetHb formation was monitored as mentioned before. The results showed that in both in vitro and in vivo models, SDH successfully attenuates Hb oxidation after challenge with sodium nitrite; this protective effect was not related to the stage of the catalytic stage of Hb oxidation, though the effect was more prominent when the compound was administered before nitrite. In conclusion, SDH can effectively, in concentration-dependent pattern, attenuate sodium nitrite-induced Hb oxidation and maintain integrity of red blood cells both in vitro and in vivo.

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Section: Controlmentioning

confidence: 98%

Free radical scavenging activity of silibinin in nitrite-induced hemoglobin oxidation and membrane fragility models

Marouf

Zalzala

Al-Khalifa

et al. 2011

Saudi Pharmaceutical Journal

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“…1 shows that glycyrrhizin, silymarin, and ursodeoxycholic acid displayed no visible cytotoxic effects at a broad range of concentration (0.5-500 μM). Previous studies indicated that silymarin at 25 μM achieves the highest hepatoprotective effect in FL83B mouse liver cells and at a concentration exceeding 25 μM abruptly increases cell damage in rat hepatocytes (Sainz-Pardo et al 1994;Lo et al 2014). …”

Section: Microarray Analysis Of Hepatoprotectant-regulated Gene Exprementioning

confidence: 97%

Glycyrrhizin, silymarin, and ursodeoxycholic acid regulate a common hepatoprotective pathway in HepG2 cells

Hsiang

Kao

et al. 2015

Phytomedicine

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Antioxidant properties of silybin glycosides

Kosina

Křen²,

Gebhardt

et al. 2002

Phytother. Res.

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New soluble derivatives of the hepatoprotective flavonolignan silybin (1), namely silybin galactoside (2), glucoside (3), lactoside (4) and maltoside (5) were investigated for their radical scavenging and antilipoperoxidation properties. According to cyclic voltammetry the results show that glycosides are weaker electron donors than silybin, although it was of interest that they were found to be more potent scavengers of the 1,1-diphenyl-2-picrylhydrazyl and the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)-derived radicals. The glycosides (2)-(5) were more efficient than silybin in preventing tert-butylhydroperoxide-induced lipoperoxidation of rat liver mitochondrial membranes. Furthermore, glycosides (2)-(5) were significantly more cytoprotective than silybin in tert-butylhydroperoxide-damaged rat erythrocytes and primary hepatocyte cultures. Glycosylation of silybin substantially reduced its toxic effects in primary cultured hepatocytes observed during prolonged incubation. These results suggest that silybin glycosides are suitable soluble derivatives of silybin for experimental studies and may have therapeutic potential.

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The effects of silymarin on the attachment and viability of primary cultures of rat hepatocytes

Cited by 6 publications

References 5 publications

Free radical scavenging activity of silibinin in nitrite-induced hemoglobin oxidation and membrane fragility models

Free radical scavenging activity of silibinin in nitrite-induced hemoglobin oxidation and membrane fragility models

Glycyrrhizin, silymarin, and ursodeoxycholic acid regulate a common hepatoprotective pathway in HepG2 cells

Antioxidant properties of silybin glycosides

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