The effects of some carbohydrates on growth and somatic embryogenesis in citrus callus culture

Kayim, Mukaddes; Koc, N. Kemal

doi:10.1016/j.scienta.2006.01.040

Cited by 36 publications

(18 citation statements)

References 13 publications

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“…Although genotypic variation in the somatic embryogenic response has been observed in other species (Kim et al 2004;Kayim and Koc 2006), only moderate effects were observed for the three cultivars of taro examined in this study. Further studies using additional cultivars are required to determine the full extent of such effects.…”

Section: Discussioncontrasting

confidence: 49%

Somatic embryogenesis, organogenesis and plant regeneration in taro (Colocasia esculenta var. esculenta)

Deo

Harding

Taylor

et al. 2009

Plant Cell Tiss Organ Cult

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Callus was initiated in three different ''esculenta'' taro cultivars by culturing corm slices in the dark on half-strength MS medium supplemented with 2.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) for 20 days followed by subculture of all corm slices to half-strength MS medium containing 1.0 mg/l thidiazuron (TDZ). Depending on the cultivar, 20-30% of corm slices produced compact, yellow, nodular callus on media containing TDZ. Histological studies revealed the presence of typical embryogenic cells which were small, isodiametric with dense cytoplasms. Somatic embryos formed when callus was transferred to hormone-free medium and *72% of the embryos germinated into plantlets on this medium. Simultaneous formation of roots and shoots during germination, and the presence of shoot and root poles revealed by histology, confirmed that these structures were true somatic embryos. Plants derived from somatic embryos appeared phenotypically normal following 2 months growth in a glasshouse. This method is a significant advance on those previously reported for the esculenta cultivars of taro due to its efficiency and reproducibility.

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Section: Discussioncontrasting

confidence: 49%

Somatic embryogenesis, organogenesis and plant regeneration in taro (Colocasia esculenta var. esculenta)

Deo

Harding

Taylor

et al. 2009

Plant Cell Tiss Organ Cult

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“…In mutagenesis , mutagens viz., physical(gamma rays) and chemicals such as ethyl methane sulfonate (EMS) and methyl methane sulfonate (MMS) are most frequently used (Jain, 2005). Citrus is a plant that have long juvenility period and breeding of which is restricted by conventional methods due to complication of their genetic systems (Kayim and Koe, 2006). Although several citrus rootstocks have been propagated through tissue culture, very limited work are reported on in vitro multiplication of rough lemon (Chaturvedi et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Effect of mutagens on regeneration and growth of in vitro grown epicotyl segments of rough lemon seedlings (Citrus jambhiri Lush.)

Kaur

2015

JANS

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Abstract:The present study on the effect of mutagens on regeneration and growth of in vitro grown epicotyls segments of rough lemon seedlings (Citrus jambhiri Lush.) was carried out during the years 2009-2010 and 2010-2011 in the Tissue Culture Laboratory, Department of Fruit Science, Punjab Agricultural University, Ludhiana. Developmental characteristics of the in vitro grown epicotyls segments on regeneration media were treated employing gamma radiation in Gray(Gy) at 0, 5,10,15,20,25,30,35,40 and 45 Gy; the alkylating agent ethyl methane sulfonate (EMS) and methyl methane sulfonate (MMS) each at 0, 0.1, 0.2, 0.3, 0.4,0.5 and 0.6% (v/v) were evaluated. Epicotyl segments from one month old in vitro grown seedling were cultured in regeneration medium (MS+BAP 1.0 mglit -1 ) under controlled laboratory conditions (25±2⁰ C, 16 hr photoperiod, 2000 lux light). LD 50 , the dose required to kill half of the tested population corresponded to 35Gy for gamma radiation, 0.3% each for EMS and MMS treatments. Number of days taken for regeneration increased with increasing dose of gamma irradiation, EMS and MMS. Percent regeneration, number of buds, number of shoots, shoot length, number of leaves, internodal length, primary root length and number of secondary roots decreased with increasing dose of gamma radiation , EMS and MMS. The study would be beneficial to induce desirable variations in plant growth characteristics of rough lemon by the use of mutagens treatment.

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“…Distinctive from other plants, SE of citrus embryogenic calluses (EC) requires carbohydrates like glycerol rather than hormones (Kayim and Koc 2006). It is reported that endogenous polyamine level (Wu et al 2009) is relevant to SE capability maintenance of citrus EC.…”

Section: Introductionmentioning

confidence: 99%

Stage and tissue-specific modulation of ten conserved miRNAs and their targets during somatic embryogenesis of Valencia sweet orange

Liu

et al. 2010

Planta

114

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Somatic embryogenesis (SE) is a remarkable process of plant somatic cells developing into an embryo capable of forming a complete plant. MiRNAs play important roles in plant development by regulating expression of their target genes, but its function in SE has rarely been studied. Herein, ten conserved miRNAs with critical functions in plant development are detected by stem-loop qRT-PCR in the SE system of Valencia sweet orange. Sixteen unigenes from citrus are predicted to be targeted by six of the miRNAs. Cleavage sites on 15 of these target mRNAs are mapped by 5'RACE, of which ten are reported in this study. Transcript abundances of the 16 target unigenes are detected by qRT-PCR during SE process. Stage and tissue-specific expressions of miRNAs and their targets suggest their possible modulation on SE of citrus callus: miR156, 168 and 171 exert regulatory function during somatic embryo induction process; miR159, 164, 390 and 397 are related to globular-shaped embryo formation; miR166, 167 and 398 are required for cotyledon-shaped embryo morphogenesis; in addition, target genes of miR164, 166 and 397 are associated with SE disability of nonembryogenic callus. Exploration of miRNA-mediated modulation on SE is expected to provide new insights into plant cell totipotency, as well as how to maintain and enhance the embryogenic capacity of somatic cells.

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The effects of some carbohydrates on growth and somatic embryogenesis in citrus callus culture

Cited by 36 publications

References 13 publications

Somatic embryogenesis, organogenesis and plant regeneration in taro (Colocasia esculenta var. esculenta)

Somatic embryogenesis, organogenesis and plant regeneration in taro (Colocasia esculenta var. esculenta)

Effect of mutagens on regeneration and growth of in vitro grown epicotyl segments of rough lemon seedlings (Citrus jambhiri Lush.)

Stage and tissue-specific modulation of ten conserved miRNAs and their targets during somatic embryogenesis of Valencia sweet orange

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