The Effects of some Polycyclic Hydrocarbons on Mouse Liver -SH Levels

Calcutt, G.; Doxey, D. L.; Coates, Joan

doi:10.1038/bjc.1959.80

Cited by 3 publications

(3 citation statements)

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“…Glutathione estimations have been done in a few cases during the experiments with N.2 fluoroenylacetamide and p-dimethylaminoazobenzene. There the evidence would imply deletion of sulphydryl as was previously found by Calcutt et al (1959) for the case of anthracene and liver tissue. In this particular case it is known that mercapturate formation plays a part in the metabolism of anthracene, but there is no evidence available of mercapturate formation after treatment with urethane.…”

supporting

confidence: 63%

“…Recently, Calcutt and Doxey (1959) described a simple technique for tissue -SH measurements using small weights of tissue. This technique was then used to determine the effects of various carcinogenic and non-carcinogenic hydrocarbons on the liver -SH values of mice previously treated with one of these hydrocarbons (Calcutt, Doxey and Coates, 1959). This work dealt with the relationships of-SH levels to the metabolism of the agents in question, since all are known to be metabolised in the liver but none can be regarded as a true hepato-carcinogen.…”

mentioning

confidence: 99%

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The Effects of Some Chemical Carcinogens upon the -SH Levels of Target and Non-Target Tissues

Calcutt¹,

Doxey²,

Coates³

1960

Br J Cancer

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THE role of sulphydryl (-SH) groups in both tumour growth and the induction of tumours has been a subject of interest for many years. Actual experimental work in this field has been limited and often only via indirect approaches. Methods of measuring the -SH within tissues have been unsatisfactory and in many cases involved tedious procedures with specialised equipment. Recently, Calcutt and Doxey (1959) described a simple technique for tissue -SH measurements using small weights of tissue. This technique was then used to determine the effects of various carcinogenic and non-carcinogenic hydrocarbons on the liver -SH values of mice previously treated with one of these hydrocarbons (Calcutt, Doxey and Coates, 1959). This work dealt with the relationships of-SH levels to the metabolism of the agents in question, since all are known to be metabolised in the liver but none can be regarded as a true hepato-carcinogen.The present paper records a study of the effects of known chemical carcinogens on the -SH levels of susceptible and non-susceptible tissues. We have been influenced in this choice of subject by knowledge of two previous papers which bear upon this field. Boyland and Mawson (1938) found that intraperitoneal injection of 3: 4: 5: 6 dibenzocarbazole (a known hepato-carcinogen) causes a considerable rise in liver glutathione which is persistent over several months. The livers of mice injected with methylcholanthrene or 1 : 2: 5: 6 dibenzanthracene (neither being a liver carcinogen) did not show a similar increase in glutathione. Di Paolo and Niedbala (1957) found that after a single painting with either 1: 2: 5: 6 dibenzanthracene or 9:10 dimethylbenzanthracene the -SH content of mouse skin was raised above the normal value and this increase was persistent for at least five days. The chemically related, but non-carcinogenic compound, anthracene, was found to have no effect on skin -SH levels.

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confidence: 63%

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The Effects of Some Chemical Carcinogens upon the -SH Levels of Target and Non-Target Tissues

Calcutt¹,

Doxey²,

Coates³

1960

Br J Cancer

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“…The sulphydryl content of 40 samples of the HeLa cells, measured by the method of Calcutt and Doxey (1959) was 10 1 ± 19 jug. per 100 mg. wet weight.…”

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confidence: 99%