The peak fat oxidation rate (PFO) and the exercise intensity that elicits PFO (Fat max) are associated with endurance performance during exercise primarily involving lower body musculature, but it remains elusive whether these associations are present during predominant upper body exercise. The aim was to investigate the relationship between PFO and Fat max determined during a graded exercise test on a ski-ergometer using double-poling (GET-DP) and performance in the long-distance crosscountry skiing race, Vasaloppet. Forty-three healthy men completed GET-DP and Vasaloppet and were divided into two subgroups: recreational (RS, n = 35) and elite (ES, n = 8) skiers. Additionally, RS completed a cycle-ergometer GET (GET-Cycling) to elucidate whether the potential relationships were specific to exercise modality. PFO (r 2 = .10, P = .044) and Fat max (r 2 = .26, P < .001) were correlated with performance; however, V O 2peak was the only independent predictor of performance (adj. R 2 = .36) across all participants. In ES, Fat max was the only variable associated with performance (r 2 = .54, P = .038). Within RS, DP V O 2peak (r 2 = .11, P = .047) and ski-specific training background (r 2 = .30, P = .001) were associated with performance. Between the two GETs, Fat max (r 2 = .20, P = .006) but not PFO (r 2 = .07, P = .135) was correlated. Independent of exercise mode, neither PFO nor Fat max were associated with performance in RS (P > .05). These findings suggest that prolonged endurance performance is related to PFO and Fat max but foremost to V O 2peak during predominant upper body exercise. Interestingly, Fat max may be an important determinant of performance among ES. Among RS, DP V O 2peak , and skiing experience appeared as performance predictors. Additionally, whole-body fat oxidation seemed specifically coupled to exercise modality. K E Y W O R D S crosscountry skiing, double-poling, peak fat oxidation, prolonged endurance performance | 2045 RØMER Et al. Note: Data are presented as mean ± 95% CI and range. Abbreviations: BMI, body mass index; FFM, fat-free mass; UBMM, upper body muscle mass (arm + torso). a n = 42 and n = 7 for all participants and ES, respectively.