The effects of subcutaneous and intraocular administration of class B ODN CpG in chicken on the expression of TLR21, IFN-γ and IL-1β

Chrząstek, Klaudia; Wieliczko, Alina

doi:10.2478/pjvs-2014-0089

Cited by 3 publications

(2 citation statements)

References 32 publications

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“…Interestingly, TLRs recognizing others PAMPs are also upregulated, such as TLR2, TLR3, TLR4, TLR5, and TLR6. Of note, TLR21, the chicken TLR9, was detected but surprisingly not differentially expressed ( Chrząstek and Wieliczko, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

An integrated transcriptomics and metabolomics study of the immune response of newly hatched chicks to the cytosine-phosphate-guanine oligonucleotide stimulation

et al. 2020

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The immunological immaturity of the innate immune system during the first-week post-hatch enables pathogens to infect chickens, leading to the death of the animals. Current preventive solutions to improve the resistance of chicks to infections include vaccination, breeding, and sanitation. Other prophylactic solutions have been investigated, such as the stimulation of animal health with immunostimulants. Recent studies showed that administration of immune-modulators to one-day-old chicks, or in ovo, significantly reduces mortality in experimental bacterial or viral infection challenge models. Owing to a lack of molecular biomarkers required to evaluate chicken immune responses and assess the efficacy of vaccines or immune-modulators, challenge models are still used. One way to reduce challenge experiments is to define molecular signatures through omics approaches, resulting in new methodologies to rapidly screen candidate molecules or vaccines. This study aims at identifying a dual transcriptomics and metabolomics blood signature after administration of CpG-ODN (cytosine-phosphate-guanine oligodeoxynucleotides), a reference immune-stimulatory molecule. A clinical study was conducted with chicks and transcriptomics and metabolomics analyses were performed on whole-blood and plasma samples, respectively. Differentially expressed genes and metabolites with different abundance were identified in chicks treated with CpG-ODN. The results showed that CpG-ODN activated the innate immune system, within hours after administration, and its effect lasted over time, as metabolomics and transcriptomics profiles still varied 6 D after administration. In conclusion, through an integrated clinical omics approach, we deciphered in part the mode of action of CpG-ODN in post-hatch chicks.

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Section: Discussionmentioning

confidence: 99%

An integrated transcriptomics and metabolomics study of the immune response of newly hatched chicks to the cytosine-phosphate-guanine oligonucleotide stimulation

et al. 2020

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“…chTLR21 expression could be induced by stimulation with CpG-ODN. Inoculation of class B CpG ODN subcutaneously potently upregulated the expression of TLR21, interleukin (IL)-1β, and interferon (IFN)-γ mRNA in the blood cells (Chrzastek and Wieliczko, 2014). Another study conducted in vitro indicated that the mRNA expression levels of TLR21 and IFN-γ were substantially increased by Class B CpG ODN stimulation at 1 h post-stimulation in a concentration-dependent manner, while this phenomenon only exited in HG cells isolated from young birds .…”

Section: Chtlr21mentioning

confidence: 99%

The Evasion of Antiviral Innate Immunity by Chicken DNA Viruses

2021

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The innate immune system constitutes the first line of host defense. Viruses have evolved multiple mechanisms to escape host immune surveillance, which has been explored extensively for human DNA viruses. There is growing evidence showing the interaction between avian DNA viruses and the host innate immune system. In this review, we will survey the present knowledge of chicken DNA viruses, then describe the functions of DNA sensors in avian innate immunity, and finally discuss recent progresses in chicken DNA virus evasion from host innate immune responses.

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Effects of Synthetic CpG Oligodeoxynucleotide K3 on Immune Response, Behavior, and Physiology in Male Layer Chicks (<i>Gallus gallus</i>)

Tachibana,

Mimura,

Khan

et al. 2024

J. Poult. Sci.

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Unmethylated cytosine-phosphate-guanine (CpG) motifs are often found in bacteria and viruses, but are rare in mammals. In mammals, CpG oligodeoxynucleotides (CpG ODN) stimulate the innate immune system via toll-like receptor 9 (TLR9). However, TLR9 is absent in birds; instead, TLR21 serves as the receptor for CpG ODN. While CpG ODN induce behavioral and physiological changes in mammals, there is limited research on their effects on behavioral and physiological parameters in birds. The aim of the present study was to determine whether intraperitoneal injection of K3, a synthetic class B CpG ODN, affected food intake, voluntary activity, cloacal temperature, blood constituents, and feed passage from the crop in chicks ( Gallus gallus ). Additionally, the effects of K3 (GC), which contains GpC motifs instead of CpG motifs, were investigated to determine the importance of these CpG motifs. Intraperitoneal injection of K3 significantly increased the mRNA expression of interleukin-1β, interleukin-6, interleukin-8, and interferon-γ in the spleen. These changes were not observed with K3 (GC) administration. Intraperitoneal injection of K3 significantly decreased food intake but did not affect voluntary activity. K3 also significantly increased cloacal temperature, tended to increase plasma glucose and corticosterone concentrations and significantly decreased feed passage from the crop. In contrast, K3 (GC) showed no effects on these parameters. These results demonstrate that class B CpG ODN is associated with anorexia, hyperthermia, and reduced feed passage through the digestive tract in chicks during bacterial and viral infections.

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The effects of subcutaneous and intraocular administration of class B ODN CpG in chicken on the expression of TLR21, IFN-γ and IL-1β

Cited by 3 publications

References 32 publications

An integrated transcriptomics and metabolomics study of the immune response of newly hatched chicks to the cytosine-phosphate-guanine oligonucleotide stimulation

An integrated transcriptomics and metabolomics study of the immune response of newly hatched chicks to the cytosine-phosphate-guanine oligonucleotide stimulation

The Evasion of Antiviral Innate Immunity by Chicken DNA Viruses

Effects of Synthetic CpG Oligodeoxynucleotide K3 on Immune Response, Behavior, and Physiology in Male Layer Chicks (<i>Gallus gallus</i>)

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