The effects of sulforaphane on canine osteosarcoma proliferation and invasion

Rizzo, Valeria; Levine, Corri B.; Wakshlag, Joseph J.

doi:10.1111/vco.12212

Cited by 12 publications

(11 citation statements)

References 49 publications

(75 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sulforaphane interacts with several cancer pathways including PI3K/AKT and MAPK/MEK/ERK in colon and prostate cancer, leukemia and multiple myeloma cell lines 9,19‐21,35 . Interestingly, several proteins identified in this study are known to interplay with these pathways.…”

Section: Discussionmentioning

confidence: 74%

“…Sulforaphane interacts with several cancer pathways including PI3K/AKT and MAPK/MEK/ERK in colon and prostate cancer, leukemia and multiple myeloma cell lines. 9,[19][20][21]35 Interestingly, several proteins identified in this study are known to interplay with these pathways. For example, lamin A/C and pleckstrin, both of which are positively associated with the PI3K/AKT pathway, [41][42][43]47,54,55 were significantly downregulated following supplementation with SFN in this study (FC D7/D0 = 0.2 and 0.4, respectively).…”

Section: Gene Set Enrichment Analysismentioning

confidence: 79%

“…A bioavailability profile of SFN in healthy dogs is comparable to humans, with a significant decrease in HDAC activity observed 24 hours after consumption of 1 dose of SFN 34 . Sulforaphane also reduces cell invasion and decreases focal adhesion kinase phosphorylation in canine osteosarcoma cell lines 35 . However, to our knowledge, the impact of SFN in cancer‐bearing dogs has not been explored.…”

Section: Introductionmentioning

confidence: 96%

“…34 Sulforaphane also reduces cell invasion and decreases focal adhesion kinase phosphorylation in canine osteosarcoma cell lines. 35 However, to our knowledge, the impact of SFN in cancer-bearing dogs has not been explored.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Prospective evaluation of the lymph node proteome in dogs with multicentric lymphoma supplemented with sulforaphane

Parachini‐Winter

Bracha

Ramsey

et al. 2020

Veterinary Internal Medicne

View full text Add to dashboard Cite

Background: Lymphoma (LSA) is a common malignancy in dogs. Epigenetic changes are linked to LSA pathogenesis and poor prognosis in humans, and LSA pathogenesis in dogs. Sulforaphane (SFN), an epigenetic-targeting compound, has recently gained interest in relation to cancer prevention and therapy. Objective: Examine the impact of oral supplementation with SFN on the lymph node proteome of dogs with multicentric LSA. Animals: Seven client-owned dogs with multicentric LSA. Methods: Prospective, nonrandomized, noncontrolled study in treatment-naïve dogs with intermediate or large cell multicentric LSA. Lymph node cell aspirates were obtained before and after 7 days of oral supplementation with SFN, and analyzed via label-free mass spectrometry, immunoblots, and Gene Set Enrichment Analysis. Results: There was no clinical response and no adverse events attributed to SFN. For individual dogs, the expression of up to 650 proteins changed by at least 2-fold (range, 2-100) after supplementation with SFN. When all dogs where analyzed together, 14 proteins were significantly downregulated, and 10 proteins were significantly upregulated after supplementation with SFN (P < .05). Proteins and gene sets impacted by SFN were commonly involved in immunity, response to oxidative stress, gene transcription, apoptosis, protein transport, maturation and ubiquitination. Conclusions and Clinical Importance: Sulforaphane is associated with major changes in the proteome of neoplastic lymphocytes in dogs.

show abstract

Section: Discussionmentioning

confidence: 74%

Section: Gene Set Enrichment Analysismentioning

confidence: 79%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Prospective evaluation of the lymph node proteome in dogs with multicentric lymphoma supplemented with sulforaphane

Parachini‐Winter

Bracha

Ramsey

et al. 2020

Veterinary Internal Medicne

View full text Add to dashboard Cite

show abstract

“…Both studies showed that Vorinostat was able to induce histone H3 acetylation in these canine cancer cells. The effects of another HDACi, sulforaphane, has been shown on canine osteosarcoma cells, significantly decreasing cell invasion and downregulating focal adhesion kinase (FAK) signaling (203).…”

Section: Histone Deacetylase Inhibitors (Hdaci)mentioning

confidence: 99%

Epigenetic Mechanisms in Canine Cancer

2020

View full text Add to dashboard Cite

A plethora of data has highlighted the role of epigenetics in the development of cancer. Initiation and progression of different cancer types are associated with a variety of changes of epigenetic mechanisms, including aberrant DNA methylation, histone modifications, and miRNA expression. At the same time, advances in the available epigenetic tools allow to investigate and reverse these epigenetic changes and form the basis for the development of anticancer drugs in human oncology. Although human and canine cancer shares several common features, only recently that studies emerged investigating the epigenetic landscape in canine cancer and applying epigenetic modulators to canine cancer. This review focuses on the existing studies involving epigenetic changes in different types of canine cancer and the use of small-molecule inhibitors in canine cancer cells.

show abstract

Sulforaphane absorption and histone deacetylase activity following single dosing of broccoli sprout supplement in normal dogs

Curran

Bracha

Wong

et al. 2018

Veterinary Medicine & Sci

View full text Add to dashboard Cite

The role of epigenetic alterations during cancer has gained increasing attention and has resulted in a paradigm shift in our understanding of mechanisms leading to cancer susceptibility. Sulforaphane (SFN) is a naturally occurring isothiocyanate derived from the precursor glucosinolate, glucoraphanin (GFN), which is found in cruciferous vegetables such as broccoli. Sulforaphane has been shown to suppress tumour growth by several mechanisms including inhibiting histone deacetylases. The objective of this study was to provide a detailed analysis of sulforaphane absorption following a single oral dose of a broccoli sprout supplement in normal dogs. A single dose of broccoli sprout supplement (with active myrosinase) was orally administered to 10 healthy adult dogs. Blood and urine samples were collected prior to dosing, and at various time points post‐dosing. Plasma total SFN metabolite levels peaked at 4 h post‐consumption and were cleared by 24 h post‐consumption. Urinary SFN metabolites peaked at 4 h post‐consumption, and remained detectable at 24 and 48 h post‐supplement consumption. A trend for decrease in histone deacetylase activity was observed at 1 h post‐consumption and a significant decrease was observed at 24 h post‐consumption. The data presented herein indicate that oral SFN is absorbed in dogs, SFN metabolites are detectable in plasma and urine post‐dosing, and SFN and its metabolites have some effect on histone deacetylase activity following a single dose.

show abstract

The effects of sulforaphane on canine osteosarcoma proliferation and invasion

Cited by 12 publications

References 49 publications

Prospective evaluation of the lymph node proteome in dogs with multicentric lymphoma supplemented with sulforaphane

Prospective evaluation of the lymph node proteome in dogs with multicentric lymphoma supplemented with sulforaphane

Epigenetic Mechanisms in Canine Cancer

Sulforaphane absorption and histone deacetylase activity following single dosing of broccoli sprout supplement in normal dogs

Contact Info

Product

Resources

About