The effects of superovulation of donor sows on ovarian response and embryo development after nonsurgical deep-uterine embryo transfer

Ángel, Miguel; Gil, M.A.; Cuello, C.; Sanchez-Osorio, J.; Gomis, J.; Parrilla, Inmaculada; Vilà, Jordi; Colina, I.; Dı́az, Mònica; Reixach, Josep Peñuelas i; Vázquez, J.M.; Vázquez, Juan M.; Roca, José María López; Rodríguez‐Martínez, H.

doi:10.1016/j.theriogenology.2013.12.017

Cited by 27 publications

(54 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, comparing both groups, Synthetic line showed a lower percentage of normal embryos (95 vs. 98%) and a higher percentage of abnormal embryos (5 vs. 2%). It appears that an increasing ovulation rate is accompanied by an increase in number of abnormal embryos due to decreasing quality of oocytes, which is in agreement with other studies (Angel et al, 2014;Peiró et al, 2014). In fact, it has been shown that embryonic losses, number of oocytes and degenerated embryos are increased as ovulation rate rises (Angel et al, 2014).…”

Section: Discussionsupporting

confidence: 91%

Ovulation rate and early embryonic survival rate in female rabbits of a synthetic line and a local Algerian population

et al. 2016

View full text Add to dashboard Cite

A higher litter size at birth has been reported in female rabbits from a Synthetic line than in those of the Local Algerian population. The aim of this work was to analyse whether this difference in litter size was due to a higher ovulation rate and/or embryonic survival rate in Synthetic line than in Local Algerian population. In total, 24 multiparous female rabbits from Synthetic line and 23 from Local population were used in this experiment. Litter size at birth was recorded up to the first 3 parities. Litter size was 20% higher in Synthetic line than Local population. At their 4 th gestation, the females were euthanized at 72 h post coitum. Synthetic line females had 50% more ova and embryos than those of Local population (+4.42 ova and +3.92 embryos, respectively). Synthetic line displayed a lower percentage of normal embryos and a larger number of unfertilized oocytes than Local population (-2.81% and +0.64 oocytes, respectively), but differences were not relevant. Synthetic line showed a lesser embryonic stage of development at 72 h post coitum, showing a higher percentage of early morulae (31.50 vs. 8.50%) and a lower percentage of compact morulae (51.45 vs. 78.65%) than Local population. No relevant difference was found for early embryonic survival rate between Synthetic line and Local population. In conclusion, the difference in litter size was mainly due to a higher ovulation rate in the Synthetic line, allowing more embryos to develop in this line.

show abstract

Section: Discussionsupporting

confidence: 91%

Ovulation rate and early embryonic survival rate in female rabbits of a synthetic line and a local Algerian population

et al. 2016

View full text Add to dashboard Cite

show abstract

“…These data, together with the excellent fertility results following NsDU-ET [2][3][4][5][6], suggest that the insertion of the catheter does not cause significant damage to the cervix and/or uterine horn. The results of the present study parallel earlier studies and reinforce the suggestion that NsDU-ET is a non-invasive and welfare-preserving technology.…”

Section: Discussionmentioning

confidence: 65%

“…All NsDU-ETs were performed in weaned recipients that started estrus 0 or 24 h after the donors using a previously described method [2][3][4]. Briefly, six hours prior to ET, each recipient received a single intramuscular injection of a long-acting amoxicillin suspension (Clamoxyl LA ® ) at a dosage of 15 mg/kg body weight.…”

Section: Non-surgical Deep Uterine Embryo Transfermentioning

confidence: 99%

“…With the recent development of non-surgical deep uterine (NsDU)-ET technology, the commercial use of ET in pigs is now possible. The NsDU-ET technique is a reliable procedure that yields good fertility levels when coupled to fresh embryos [1][2][3] or stored embryos, either short-term [4] or long-term [5].…”

Section: Introductionmentioning

confidence: 99%

“…Several factors affecting the success of the NsDU-ET procedure, including superovulation of the donors, synchrony between donors and recipients, use of fresh, short-term or long-term stored embryos, number of embryos transferred per recipient and the recipient's parity, have all been recently assessed [2][3][4][5][6]. However, other factors not directly related to the success of ET have to be quantified to properly estimate the final costs of porcine ET, a most relevant factor for the successful establishment of 3 commercial ET programs.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Surgical embryo collection but not nonsurgical embryo transfer compromises postintervention prolificacy in sows

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

Developmental competence of porcine genome‐edited zygotes

Gil

Martínez

Nohalez

et al. 2017

Molecular Reproduction Devel

View full text Add to dashboard Cite

Genome editing in pigs has tremendous practical applications for biomedicine. The advent of genome editing technology, with its use of site-specific nucleases-including ZFNs, TALENs, and the CRISPR/Cas9 system-has popularized targeted zygote genome editing via one-step microinjection in several mammalian species. Here, we review methods to optimize the developmental competence of genome-edited porcine embryos and strategies to improve the zygote genome-editing efficiency in pigs. K E Y W O R D SCRISPR/Cas9, cytoplasmic microinjection, in vitro-produced zygotes, in vivo-derived zygotes, TALEN, ZFNOne-step production of genetically modified pigs . . . is simple and efficient, and holds the potential to be a powerful tool for genome manipulation for biomedical and biotechnological applications.

show abstract

The effects of superovulation of donor sows on ovarian response and embryo development after nonsurgical deep-uterine embryo transfer

Cited by 27 publications

References 39 publications

Ovulation rate and early embryonic survival rate in female rabbits of a synthetic line and a local Algerian population

Ovulation rate and early embryonic survival rate in female rabbits of a synthetic line and a local Algerian population

Surgical embryo collection but not nonsurgical embryo transfer compromises postintervention prolificacy in sows

Developmental competence of porcine genome‐edited zygotes

Contact Info

Product

Resources

About