The Effects of Synthetic Gonadotrophin Releasing Factor on the Release of Luteinizing Hormone and Follicle-Stimulating Hormone From Ovine Pituitary Tissue in Vitro

Crighton, D. B.

doi:10.1677/joe.0.0580387

Cited by 7 publications

(2 citation statements)

References 10 publications

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“…The height and duration of the LH peaks induced by administration of LH-RH are known to be much less than those of the natural peak before ovulation (Crighton et al, 1974). Individual variation in the height of LH peaks was too great to allow correlation between the occurrence of apparent ovulation and the height of the LH peak Crighton & Foster (1972), Crighton (1972) and Jonas et al (1973) which show that LH-RH will induce release of FSH in vitro and in vivo.…”

Section: Discussionmentioning

confidence: 99%

Progesterone Levels Following Treatment of Seasonally Anoestrous Ewes With Synthetic Lh-Releasing Hormone

Haresign¹,

Foster²,

Haynes³

et al. 1975

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114

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Summary. Plasma progesterone determinations were carried out on blood samples collected daily from Clun Forest ewes during the normal oestrous cycle and also after administration of LH-releasing hormone (LH-RH) during seasonal anoestrus.Levels of plasma progesterone at oestrus ranged from 0\m=.\1to 0\m=.\5 ng/ml and luteal phase levels from 3 to 6 ng/ml. Levels found during seasonal anoestrus were within the range of those observed at oestrus. Following treatment with LH-RH, there was an increase in the plasma LH level in all cases and ovulation occurred in twenty-three out of twenty-seven treated ewes. In the animals which ovulated, the plasma progesterone concentration either remained basal (eighteen animals) or rose to a lower level (<2 ng/ml) than that found during the luteal phase of the cycle.

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Section: Discussionmentioning

confidence: 99%

Progesterone Levels Following Treatment of Seasonally Anoestrous Ewes With Synthetic Lh-Releasing Hormone

Haresign¹,

Foster²,

Haynes³

et al. 1975

Reproduction

Self Cite

114

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“…The LH-releasing activity of synthetic LH-RH and related peptides was assessed by incubation of ovine pituitary tissue and biological assay of the incubation medium for LH as described previously. [42][43][44] The results are expressed in terms of NIH-LH-S17 (National Institutes of Health, Bethesda, Md.). Responses to synthetic peptides were examined at four dose levels.…”

mentioning

confidence: 99%

Synthesis and biological activity of luteinizing hormone-releasing hormone and related peptides

Dj¹,

Ad²,

Jd³

1975

J. Med. Chem.

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Syntheses of the decapeptide luteinizing hormone-releasing hormone, less thanGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2 are described. The basic properties of arginine can provide a simple repetitive isolation procedure for arginine-containing peptides. The biological activities of the decapeptide, of a range of fragments and modified fragments, and of two analogs with alteration in the series at position 4 were measured by in vitro incubation with sheep pituitary slices, measuring the liberated LH by bioassay. None of the compounds of shortened sequence were active, with the exception of less thanGlu-His-Trp which showed 1% of the decapeptide in one of four experiments. Neither [Ser(But)4]-LH-RH-nor [Leu4]-LH-RH showed significant activity indicating (despite the known activity of [Ala4]-LH-RH) the importance of this part of the structure for full biological activity.

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Immunochemical staining of the rat adenohypophysis in organ culture

1975

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Rat anterior pituitaries were cytologically studied following cultivation in organ culture, with and without the addition of hypothalamic and cortical extracts. Although five distinct cell types could be identified with classical stains in the uncultivated glands, the peroxidase-labeled antibody technique (using antibodies against STH, LTH, FSH, LH and TSH) showed that not all of the immune-specific cell types were being identified with the classical stains. This discrepancy was magnified following culture as chromophilic cells seen with classic stains decreased in number with an increase in culture time. The peroxidase technique, however, revealed that all cells remained constant in type and number regardless of time in culture. While the addition of either hypothalamic or cortical extract to the culture medium produced cytological alterations demonstrated by the classical dyes, the antibody technique showed no such alterations. Such a comparison of staining techniques emphasizes the hazards of relying solely on histological procedures to reveal the hormonal activity of the pituitary gland.

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The Effects of Synthetic Gonadotrophin Releasing Factor on the Release of Luteinizing Hormone and Follicle-Stimulating Hormone From Ovine Pituitary Tissue in Vitro

Cited by 7 publications

References 10 publications

Progesterone Levels Following Treatment of Seasonally Anoestrous Ewes With Synthetic Lh-Releasing Hormone

Progesterone Levels Following Treatment of Seasonally Anoestrous Ewes With Synthetic Lh-Releasing Hormone

Synthesis and biological activity of luteinizing hormone-releasing hormone and related peptides

Immunochemical staining of the rat adenohypophysis in organ culture

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