The effects of temperature and season of collection on the onset and duration of diapause in embryos of the annual fish <i>Nothobranchius guentheri</i>

Markofsky, Jules; Matias, Jonathan R.

doi:10.1002/jez.1402020107

Cited by 64 publications

(51 citation statements)

References 14 publications

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“…A. viarius also reached somitogenesis (10 somites or less) in 16 days (Arezo et al, 2007), while A. nigrofasciatus reached this stage in about 12 days. This discrepancy could be further enhanced if the temperatures employed were the same, since the speed of development is usually directly related to the temperature (Markofsky & Matias, 1977).…”

Section: Discussionmentioning

confidence: 99%

Incubation media affect the survival, pathway and time of embryo development in Neotropical annual fish Austrolebias nigrofasciatus (Rivulidae)

Fonseca

Volcan²,

Robaldo

2017

Journal of Fish Biology

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To analyse the survival, pathway and time of embryo development in the annual fish Austrolebias nigrofasciatus eggs were monitored in four liquid media and two damp media under experimental conditions for 130 days until their development was complete. Eggs kept in the same breeding water from oviposition remained in diapause I (DI) during all experiments. In constrast, up to the stage prior to entering diapause II (DII), the other media had no influence on development. Embryos at this stage (DII), however, show longer development time when treated in medium with water and powdered coconut shell so that about 80% of embryos remained in DII at 100 days. In contrast, all other treatments had a significantly lower proportion of embryos remaining in DII. When treated with Yamamoto's solution in humid media, embryos showed the fastest development. The first fully developed embryos (DIII) were seen at 27 days after oviposition. It took an average of 46-58 days for 50% of eggs in each treatment to reach DIII. Compared with other studies, survival in all incubation media was high at between 70 and 98%. Taken together, it can be concluded that all incubation media were found to be viable for maintaining embryos. Altering developmental trajectories through the manipulation of diapauses in different media makes this species a potential model organism for laboratory studies.

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Section: Discussionmentioning

confidence: 99%

Incubation media affect the survival, pathway and time of embryo development in Neotropical annual fish Austrolebias nigrofasciatus (Rivulidae)

Fonseca

Volcan²,

Robaldo

2017

Journal of Fish Biology

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“…Embryos can enter a facultative developmental arrest or diapause at three distinct stages of development, which allow them to survive the variable durations of the dry seasons. Little is known about the mechanisms that trigger the onset and duration of each diapause, except that in Nothobranchius guentheri low temperature and low oxygen concentration increase the proportion of embryos entering diapauses (Peters, 1963;Markofsky and Matias, 1977).…”

Section: Introductionmentioning

confidence: 99%

A microinjection protocol for the generation of transgenic killifish (Species: Nothobranchius furzeri)

Hartmann

Englert

2012

Developmental Dynamics

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Background: A challenge in age research is the absence of short-lived vertebrate model organisms. The turquoise killifish Nothobranchius furzeri has an exceptionally short lifespan of 4-10 months depending on the strain. Thus, it possesses the shortest known maximum lifespan of a vertebrate species that can be bred in captivity. Results: Here we show the successful introduction of DNA and RNA molecules into the one-cell embryo of N. furzeri. For this purpose, we adapted existing microinjection protocols to inject through the remarkably thick and robust chorion of N. furzeri's eggs. The injected DNA transgene was integrated into the genome and transmitted to subsequent generations as indicated by the expression of the fluorophore enhanced green fluorescent protein (EGFP). Furthermore, we could confirm a special phase during embryonic development in which embryogenesis occurs within a re-aggregated mass of previously dispersed cells as it has been described for other related cyprinodont fish species. Conclusions: The transgenesis protocol described here provides a basis for a variety of genetic manipulations including overexpression of genes and determining their effects on lifespan and longevity. The feasibility to perform transgenesis is an important step to establish N. furzeri as a new model in age research.

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“…Inglima et al (1981) and Levels et al (1986b) reported that embryonic development with its diapauses is sensitive to substances produced and released into the water by the adult fishes. Among the exogenous (environmental) factors, capable of affecting diapauses, are oxygen availability (Peters 1963, Levels et al 1986a, dehydration of the substrate (Peters 1963, Wourms 1972c, Matias 1982) and temperature (Markofsky & Matias 1977a, Matias & Markofsky 1978. Season-dependent variations in diapause frequency were also found (Markofsky & Matias 1977a).…”

Section: Introductionmentioning

confidence: 99%

Intrinsic variability in the frequency of embryonic diapauses of the annual fish Nothobranchius korthausae, regulated by light:dark cycle and temperature

Levels¹,

Denucé²

1988

Environ Biol Fish

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SynopsisThe influence of the 1ight:dark cycle and temperature on the embryonic development, especially diapause, of the annual fish Nothobranchius korthausae was investigated. The variability of the frequency of diapause II during constant L:D cycle and temperature, but at different times of the year, was also studied. In agreement with previous studies it appeared that diapause I does practically not occur in N. korthausae (and other Nothobranchius species), even under sub-optimal conditions which are known to induce diapause II and III. Only at very low temperatures, a first developmental arrest could be induced during the dispersed phase, as well as during the reaggregation phase. Diapause II and III can be induced more easily, by exposing the embryos to low (sub-optimal) temperatures and short light periods (or constant darkness). Both diapauses are inhibited or terminated at high temperature and long light periods (12L:12D). The occurrence of an indirect 1ight:dark response via the adult fishes could not be demonstrated. At moderate temperatures and in constant darkness (or short light periods) considerable variability in diapause II-frequency was observed. This could be an intrinsic feature of annual fish development, constituting a strategy for better survival of the species.

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The effects of temperature and season of collection on the onset and duration of diapause in embryos of the annual fish Nothobranchius guentheri

Cited by 64 publications

References 14 publications

Incubation media affect the survival, pathway and time of embryo development in Neotropical annual fish Austrolebias nigrofasciatus (Rivulidae)

Incubation media affect the survival, pathway and time of embryo development in Neotropical annual fish Austrolebias nigrofasciatus (Rivulidae)

A microinjection protocol for the generation of transgenic killifish (Species: Nothobranchius furzeri)

Intrinsic variability in the frequency of embryonic diapauses of the annual fish Nothobranchius korthausae, regulated by light:dark cycle and temperature

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