The effects of temperature and salinity on 17-α-ethynylestradiol uptake and its relationship to oxygen consumption in the model euryhaline teleost (Fundulus heteroclitus)

Blewett, Tamzin A.; MacLatchy, Deborah L.; Wood, Chris M.

doi:10.1016/j.aquatox.2012.04.009

Cited by 45 publications

(18 citation statements)

References 88 publications

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“…These findings suggest that the chgH:rfp transgenic marine medaka system could be applied for detection of waterborne EE2 at a wide range of salinities, which is in agreement with our previous observations with E2 exposure (Cho et al, 2013). However, although the general applicability of this transgenic approach to variable salinities could be established, a more accurate quantitative comparison of the effects of salinity might also be needed since salinity conditions have been reported to affect the EE2 uptake by the euryhaline teleost, killifish Fundulus heteroclitus (Blewett et al, 2013). In addition, it has been reported that marine medaka fry acclimated to either freshwater or seawater possessed differential basal levels of choriogenin H transcripts (Lee et al, 2012b).…”

Section: Ee2-induced Transgene Expression Under Different Salinity Cosupporting

confidence: 77%

Expression Patterns of the chgH:rfp Transgene in Response to 17α-Ethinylestradiol (EE2) Exposure in Marine Medaka Oryzias dancena

Nam¹,

Cho²,

Kim³

2015

Fisheries and aquatic sciences

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The functional utility of a transgenic marine medaka Oryzias dancena strain carrying the red fluorescent protein (RFP) gene driven by an endogenous choriogenin H (chgH) promoter was evaluated for its ability to detect waterborne 17α-ethinylestradiol (EE2), a synthetic estrogen derivative. The chgH:rfp transgenic marine medaka larvae showed an age-dependent tendency in the efficiency of EE2-mediated transgene expression, in which transgenic larvae older than 6 days post-hatching displayed a more effective response in their transgene expression to EE2 than did younger hatchlings. During experimental exposures to high concentrations of EE2 (200 to 1,000 ng/L), the transgenic responses in the hatchlings were broadly dose-and duration-dependent. With exposures using lower doses of EE2 (25, 50 and 100 ng/L), EE2-induced transgenic RFP was also observed in the transgenic larvae, although the lower doses required exposure of longer duration. Under the EE2 exposure and microscope assay conditions used in our study, transgenic marine medaka larvae exhibited a similar degree of EE2-mediated RFP phenotype expression at various salinity levels (0, 15 and 30 ppt).

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Section: Ee2-induced Transgene Expression Under Different Salinity Cosupporting

confidence: 77%

Expression Patterns of the chgH:rfp Transgene in Response to 17α-Ethinylestradiol (EE2) Exposure in Marine Medaka Oryzias dancena

Nam¹,

Cho²,

Kim³

2015

Fisheries and aquatic sciences

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“…For liver samples (n ¼ 37), although over half of detected steroids (AED, T, 19-NT, CRN, CPTA, 17a-HPA and MGT) were statistically related to NTND (p < 0.05), the correlation was not found between NTND and the total steroids (Table S15). The apparent differences in distribution of steroids among these four tissues were probably due to different chemical properties (Table S1), different exposure concentration (Table 1), uptake rates (Blewett et al, 2013) and metabolic processes (Jurgella et al, 2006;Scott et al, 2014). As deduced from this study, good correlations between single steroid and other individual steroid/total steroids in fish plasma and muscle samples could give valuable information on distribution of steroids in fish tissues.…”

Section: Chemical Indicators Of Steroids In Fish Tissuesmentioning

confidence: 74%

Steroid bioaccumulation profiles in typical freshwater aquaculture environments of South China and their human health risks via fish consumption

Liu

et al. 2017

Environmental Pollution

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“…However, sewage effluents generally contain a mixture of various complex organic compounds including other contaminants and other pharmaceuticals and personal care products, [3,17,25,34] which may influence the bioconcentration potential of individual pharmaceuticals. [35] Furthermore, it is important to remember that differences in temperature and water properties, [36] and in fish size, age and condition [6] may complicate comparisons across species and studies because these factors have the potential to influence the level of bioconcentration. In our study, blood-plasma concentrations were the highest, and significantly higher than in the brain and liver samples.…”

Section: Discussionmentioning

confidence: 99%

Tissue-specific uptake of the benzodiazepine oxazepam in adult Eurasian perch (Perca fluviatilis)

et al. 2016

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Environmental context. Although pharmaceuticals are found in wild fish, there is no consensus as to which fish tissue to use when measuring the pharmaceutical concentrations. Using the psychiatric pharmaceutical oxazepam in Eurasian perch as an example, we found that oxazepam was distributed in the perch tissues in the following order of concentration: muscle , liver ¼ brain , blood plasma. These results help to interpret and compare previous studies, and provide guidance for future studies.Abstract. Psychoactive substances are used worldwide and constitute one of the common groups of pharmaceutical contaminants in surface waters. Typically, in field surveys and laboratory studies, muscle or whole-body homogenates are used to quantify pharmaceutical concentrations in biota, although uptake of pharmaceuticals may be tissue-specific. Therefore, the aim of this study was to investigate the tissue-specific (muscle, liver, brain and blood plasma) uptake of the anxiolytic oxazepam in adult Eurasian perch (Perca fluviatilis). In laboratory experiments, perch were exposed to four different concentrations (2, 4, 12 and 20 mgL À1 ) of oxazepam for 6 days, and muscle, liver, brain tissue and blood plasma were sampled to determine tissue-specific bioconcentration. We found that the tissue-specific bioconcentration was independent of oxazepam concentration. However, among tissue types, bioconcentration was significantly different, with the concentration in muscle , liver ¼ brain , blood plasma. Hence, it is important to consider the type of tissue used to quantify pharmaceutical uptake in fish, for predictions of species-specific sensitivity and comparisons across studies. Furthermore, our results indicate a somewhat lower transportability (brain/plasma ratio 0.54) of oxazepam from blood to brain in fish compared with in mammals, which should be kept in mind when employing 'read-across' approaches.

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The effects of temperature and salinity on 17-α-ethynylestradiol uptake and its relationship to oxygen consumption in the model euryhaline teleost (Fundulus heteroclitus)

Cited by 45 publications

References 88 publications

Expression Patterns of the chgH:rfp Transgene in Response to 17α-Ethinylestradiol (EE2) Exposure in Marine Medaka Oryzias dancena

Expression Patterns of the chgH:rfp Transgene in Response to 17α-Ethinylestradiol (EE2) Exposure in Marine Medaka Oryzias dancena

Steroid bioaccumulation profiles in typical freshwater aquaculture environments of South China and their human health risks via fish consumption

Tissue-specific uptake of the benzodiazepine oxazepam in adult Eurasian perch (Perca fluviatilis)

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