The effects of temperature changes on the isozyme and Hsp70 levels of the Amur sturgeon, Acipenser schrenckii, at two acclimation temperatures

Luo, Liang; Zhao, Zhigang; Zhang, Rui; Guo, Kun; Wang, Shihui; Xu, Wei; Wang, Chang‐An

doi:10.1016/j.aquaculture.2021.737743

Cited by 9 publications

(6 citation statements)

References 41 publications

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“…Similarly, acclimation to cold temperatures resulted in a reduction in the specific concentration of LDH in cod (G. morhua) liver and white muscle (Zakhartsev et al 2004). However, contrary to our results, exposure of Amur sturgeon (A. schrenckii) to heat shock (23C) decreased the intensity of a specific LDH-2 band during the 72-hour exposure period (Luo et al 2022), indicating a species-specific response to thermal stress.…”

Section: Heart 4 Discussioncontrasting

confidence: 99%

“…The findings of this study are similar to those reported for LDH in the loach Misgurnus fossilis (Ozernyuk et al 1994) and crucian carp Carassius carassius (Vornanen 1994) during seasonal temperature changes and in the cod Gadus morhua acclimated to 4°C and 12°C (Zakhartsev et al 2004). Recently, Luo et al (2022) showed that exposure to (13C) or heat (23C) shock did not influence the LDH isozyme expression pattern in Amur sturgeon (Acipenser schrenckii). Aswani and Trabucco (2019) observed that the two isozymes of brain AChE (a 59 Kda monomer and a 244 Kda tetramer) in Tilapia mossambica acclimated to 25 and 37°C had similar electrophoretic mobility and intensity but differed in their Kms, thermal stability, and activation energy, and suggested that the temperature adaptation of brain AChE in T. mossambica involves the aggregation-dissociation of the two isozymes.…”

Section: Heart 4 Discussionsupporting

confidence: 91%

“…Similarly, quantitative changes in liver G6PDH were detected by immunodetection technique during cold acclimation in I. punctatus (Seddon 1997). An alternative to the quantitative theory is the synthesis of qualitatively distinct molecules, in which the warm and cold-acclimated fish would differ mainly in the proportion of the genotype being expressed, such that the populations express isozymes that are qualitatively different (Schulte 2004;Luo et al 2022). A direct correlation between isozyme expression and temperature acclimation was reported in the brain acetylcholinesterase (AChE) of the trout (Salmo gairdnerii) with two variants, namely warm-variant and cold-variant, of which only the warm-variant occurred during warm acclimation (17°C) and the cold-variant occurred only during cold acclimation (2°C), while both isozymes (variants) were present during acclimation at an intermediate temperature (12°C) (Baldwin and Hochachka 1970).…”

Section: Introductionmentioning

confidence: 99%

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Acclimation to warm temperatures modulates lactate and malate dehydrogenase isozymes in juvenile Horabagrus brachysoma (Günther)

Dalvi,

Pal,

Debnath

2023

J Exp Bio & Ag Sci

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Differential expression of isozymes enables fish to tolerate temperature fluctuations in their environment. The present study explores the modulation of lactate dehydrogenase (LDH) and cytoplasmic malate dehydrogenase (sMDH) isozyme expression in the heart, muscle, brain, liver, gill, and kidney of juvenile Horabagrus brachysoma after 30 days of acclimation at 26, 31, 33, and 36°C. LDH and sMDH zymography were performed using native polyacrylamide gel electrophoresis. The zymography revealed five distinct bands of LDH isoenzymes (labelled from cathode to anode as LDH-A4, LDH-A3B1, LDH-A2B2, LDH-A1B3, and LDH-B4) and three distinct bands of sMDH isoenzymes (labelled from cathode to anode as sMDH-A2, sMDH-AB, and sMDH-B2), with considerable variation in their expression in the tissues. Acclimation to the test temperatures did not influence the expression patterns of LDH or sMDH isozymes. Densitometric analysis of individual isozyme bands revealed a reduction in the densities of bands containing the LDH-B and sMDH-B molecules, while the densities of bands containing the LDH-A and sMDH-A molecules increased in the gills and muscle, indicating the role of these organs in adaptive responses to thermal acclimation. However, the total densities of the LDH and sMDH isozymes increased with higher acclimation temperatures, indicating that adaptation to increased temperatures in H. brachysoma is primarily characterised by quantitative changes in isozyme expression.

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Section: Heart 4 Discussioncontrasting

confidence: 99%

Section: Heart 4 Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

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Acclimation to warm temperatures modulates lactate and malate dehydrogenase isozymes in juvenile Horabagrus brachysoma (Günther)

Dalvi,

Pal,

Debnath

2023

J Exp Bio & Ag Sci

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“…In tumors and cancer, the genes are mainly involved in melanoma, acute myeloid leukemia, liver tumor, glioma, breast cancer, colon cancer, bladder cancer, and ovarian cancer, which may suggest that temperature stimulation stress does great harm to organisms. Many studies of immune stress have focused on HSP70 , including in Japanese sturgeon ( Acipenser schrenckii ) ( Luo et al, 2022 ), Nile tilapia ( O. niloticus ) ( Iri et al, 2022 ), and the ray-finned fish Schizothorax wangchiachii ( Wang et al, 2022 ). In our study, the genes stimulated by temperature were mainly concentrated in immune, metabolic, apoptosis, and cancer-related pathways, which was similar to results reported for Atlantic salmon (heat stress) ( Nuez-Ortín et al, 2018 ), pufferfish ( Takifugu fasciatus ) (cold stress) ( Wen et al, 2019 ), and cardinal fish ( Pterapogon kauderni ) (heat and cold stress) ( Pan et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Studies on the molecular level changes and potential resistance mechanism of Coreius guichenoti under temperature stimulation

Duan

Zhou

et al. 2022

Front. Genet.

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In this study, we used transcriptome and proteome technology to analyze molecular level changes in tissues of Coreius guichenoti cultured at high temperature (HT) and low temperature (LT). We also screened for specific anti-stress genes and proteins and evaluated the relationships between them. We identified 201,803 unigenes and 10,623 proteins. Compared with the normal temperature (NT), 408 genes and 1,204 proteins were up- or down-regulated in brain tissues, respectively, at HT, and the numbers were 8 and 149 at LT. In gill tissues, the numbers were 101 and 1,745 at HT and 27 and 511 at LT. In gill tissues at both temperatures, the degree of down-regulation (average, HT 204.67-fold, LT 443.13-fold) was much greater than that of up-regulation (average, HT 28.69-fold, LT 17.68-fold). The protein expression in brain (average, up 52.67-fold, down 13.54-fold) and gill (average, up 73.02-fold, down 12.92-fold) tissues increased more at HT than at LT. The protein expression in brain (up 3.77-fold, down 4.79-fold) tissues decreased more at LT than at HT, whereas the protein expression in gill (up 8.64-fold, down 4.35-fold) tissues was up-regulated more at LT than at HT. At HT, brain tissues were mainly enriched in pathways related to metabolism and DNA repair; at LT, they were mainly enriched in cancer-related pathways. At both temperatures, gill tissues were mainly enriched in pathways related to cell proliferation, apoptosis, immunity, and inflammation. Additionally, Kyoto Encyclopedia of Genes and Genomes pathway analysis showed more differentially expressed proteins in gill tissues than in brain tissues at HT and LT, and temperature stimulation led to the strengthening of metabolic pathways in both tissues. Of the 96 genes we identified as potentially being highly related to temperature stress (59 from transcriptome and 38 from proteome data), we detected heat shock protein 70 in both the transcriptome and proteome. Our results improved our understanding of the differential relationship between gene expression and protein expression in C. guichenoti. Identifying important temperature stress genes will help lay a foundation for cultivating C. guichenoti, and even other fish species, that are resistant to HT or LT.

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“…Even under conditions of intensive culture, crucian carps have obvious advantages in terms of their antioxidant capacity and growth in aquaponics systems [15]. At the cellular level, their stress response is characterized by the increased expression of the heat shock protein (HSP) [18]. The increased synthesis of HSP70 has been reported in response to various stressors, including hypoxia, pathogens and contaminants [18,19].…”

Section: Introductionmentioning

confidence: 99%

Influences of Aquaponics System on Growth Performance, Antioxidant Parameters, Stress Parameters and Gene Expression of Carassius auratus

Mao

Wang

Zhao

et al. 2023

Fishes

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Aquaponics is a new type of composite farming system, which combines aquaculture and hydroponics through ecological design to achieve scientific synergism. However, the effects of aquaponics on the welfare status and stress parameters of fish are unclear. In this study, 150 crucian carp with an average initial body weight of 7.06 ± 0.32 g were selected. Nine fish were randomly selected as the control group (NC group and hypoxia group) for stress, antioxidant and gene expression parameters after acclimation and hypoxia stress, respectively. The remaining crucian carps were randomly divided into three experimental groups with 20 crucian carps in each group (T0, T1 and T2, respectively), with three replicates. The fresh weight of the lettuce root in the T2 group had no significant effect (p > 0.05). Compared with the control group (T0 and T1), there were significant differences in the specific growth rate, the weight gain rate, the fresh leaf weight, the chlorophyll content and the water quality parameters in group T2 (p < 0.05). Regarding the biochemical parameters, superoxide dismutase and catalase showed significant differences between the T2 and T1 groups (p < 0.05). At the same time, the HSP70 and Prdx3 genes were upregulated in the liver of the T2 group compared to the NC group and the hypoxia group. The research suggests that aquaponics may reduce the hypoxia stress of crucian carp without affecting the growth of crucian carp and lettuce.

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The effects of temperature changes on the isozyme and Hsp70 levels of the Amur sturgeon, Acipenser schrenckii, at two acclimation temperatures

Cited by 9 publications

References 41 publications

Acclimation to warm temperatures modulates lactate and malate dehydrogenase isozymes in juvenile Horabagrus brachysoma (Günther)

Acclimation to warm temperatures modulates lactate and malate dehydrogenase isozymes in juvenile Horabagrus brachysoma (Günther)

Studies on the molecular level changes and potential resistance mechanism of Coreius guichenoti under temperature stimulation

Influences of Aquaponics System on Growth Performance, Antioxidant Parameters, Stress Parameters and Gene Expression of Carassius auratus

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