The Effects of Temperature on the Susceptibility of Potato Plants to Infection and Accumulation of Potato Leafroll Virus

Abstract

Temperature both before and after aphid inoculation with potato leafroll virus (PLRV) greatly influenced the susceptibility of potato plants to infection and virus accumulation, as evaluated with ELISA using cultivars with different ratings for the resistance to PLRV. Pre‐incubation at 15 compared to 27 °C increased the susceptibility of plants to infection and a subsequent PLRV accumulation. The virus was detected by ELISA in a greater proportion of plants and reached a higher concentration, when the plants were kept at 27 than at 15 °C after inoculation. The mean ELISA values obtained with PLRV‐infected plants in the 15/27 combination of the pre‐/post‐inoculation temperatures over the period 1—6 wks after inoculation were significantly higher than those in the 27/27, 15/15 and 27/15 treatments, and the values obtained in the 27/27 treatment were significantly higher than those in the 15/15 and 27/15 ones. A hypersensitive‐like intolerance reaction to PLRV occurred in the resistant cv. Irga only in the plants kept at 27 °C after inoculation.

Temperature-Induced Viral Resistance in Emiliania huxleyi (Prymnesiophyceae)

“…Our results clearly demonstrate an elevated temperature-induced resistance to EhV infection in an array of sensitive host strains (CCMP374, CCMP1516, and DWN61/87/10). Though novel in E. huxleyi , similar temperature effects on infectivity/sensitivity have been demonstrated in the bloom-forming Rhaphidophyte, Heterosigma akashiwo -HaV system [17] , and in terrestrial plant host/virus systems such as the Potato Leafroll Virus [44] and Soybean Mosaic Virus [45] . Together with the results from our study on E. huxleyi , it is clear that temperature plays a fundamental mechanistic role in host-virus interactions.…”

Effect of temperature on the algicidal activity and the stability of HaV (Heterosigma akashiwo virus)

“…Indeed, in studies on terrestrial plant viruses, it is reported that the hosts' resistance against virus replication is changeable with temperature (Fraser & Loughlin 1982). However, f.urther examination at a molecular biological level would be required in order to elucidate effects of temperature on both infectivity of HaV and sensitivity of H. akashiwo (Aozaki et al 1989, Syller 1991. Although HaV08 was inoculated at higher m.0.i.…”

“…Especially in studies of plant viruses, temperature is considered to be a significant factor that influences the dynamics of the disease process in the host plant (Aozaki et al 1989, Mansky et al 1991, Syller 1991. For example, multiplication of the tobacco mosaic virus 'E-mail: nagasaki@nnf.affrc.go.jp O Inter- Research 1998 infecting tomato is severely reduced at high temperatures due to genetic control by the host for disease suppression (Fraser & Loughlin 1982).…”

Two allelic or tightly linked genetic factors at the PLRV.4 locus on potato chromosome XI control resistance to potato leafroll virus accumulation

“…Ten plants per clone were inoculated with PLRV using Myzus persicae (Sulz) as the virus vector, as described ). The plants were tested 5 weeks after inoculation by quantitative ELISA as described by Syller (1991). Transmission of PLRV to Physalis floridana Rydb.…”