The Effects of Tetramisole  (R 8299) on the Growing Chicken

Cyclopropenoid fatty acids (CPFAs), found in cottonseed, have been shown to have detrimental health effects to susceptible livestock. Previous quantitative analytical methods for the determination of CPFAs expressed these acids in terms of their relative abundance with respect to other fatty acids in the oil, necessitating the concurrent analysis of other fatty acids. The proposed analytical method describes the quantitation of three relevant CPFAs for cotton (malvalic acid, sterculic acid, and dihydrosterculic acid) in cottonseed in micrograms per gram fresh weight of sample. The method involves extraction of the oil, saponification, and derivatization of the free fatty acids with 2-bromoacetophenone to give the phenacyl esters. These esters are then separated by dual-column reverse-phase high-performance liquid chromatography and quantitated via external standards. This is the first method to include external calibration standards for CPFAs and, as such, is capable of direct quantification with no further data conversion required. CPFA data generated from the analysis of cottonseed, cottonseed meal, and cottonseed oil produced in the United States in 2002 are presented.

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A Quantitative Method for the Determination of Cyclopropenoid Fatty Acids in Cottonseed, Cottonseed Meal, and Cottonseed Oil (Gossypium hirsutum) by High-Performance Liquid Chromatography

Obert

Hughes

Sorenson

et al. 2007

J. Agric. Food Chem.

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Relative Deposition of the Anthelmintic Tetramisole in Eggs and Poultry Tissues

Allewijn

Marsboom

1967

Poultry Science

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The Effects of Tetramisole (R 8299) on the Growing Chicken

Cited by 2 publications

References 2 publications

A Quantitative Method for the Determination of Cyclopropenoid Fatty Acids in Cottonseed, Cottonseed Meal, and Cottonseed Oil (Gossypium hirsutum) by High-Performance Liquid Chromatography

A Quantitative Method for the Determination of Cyclopropenoid Fatty Acids in Cottonseed, Cottonseed Meal, and Cottonseed Oil (Gossypium hirsutum) by High-Performance Liquid Chromatography

Relative Deposition of the Anthelmintic Tetramisole in Eggs and Poultry Tissues

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