The effects of the centrifugation speed on the survival of autogenous fat grafts in a rat model

Bozkurt, Mehmet; Kapi, Emin; Şirinoğlu, Hakan; Güvercin, Emre; Filinte, Gaye Taylan; Filinte, Deniz

doi:10.3109/2000656x.2015.1137926

Cited by 10 publications

(3 citation statements)

References 23 publications

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“…10 Each steps has further technical details such as the length of the fat aspiration cannula, fat graft harvesting pressure, centrifugation or washing of the harvested grafts, and the technique and syringe used in the injection step. 11 The application of these technical details mostly depends on the individual experience and preference of the senior surgeons rather than evidence-based data obtained by experimental studies. 1,3,12,13 The presented study focuses on an important issue in fat harvesting and questions the necessity of tumescent solution usage before fat graft harvest.…”

Section: Discussionmentioning

confidence: 99%

Effects of Tumescent Solution on Fat Survival

et al. 2017

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Autologous fat transfer is a commonly used procedure in plastic surgery practice. The long-term survival rate of fat grafts is the most important issue for satisfactory results. The presented study includes the effects of different tumescent solutions on long-term fat graft survival. A total of 24 rats were divided into four groups: sham, lidocaine, adrenaline, and lidocaine + adrenaline groups. In all groups except the sham group, right inguinal fat pad was harvested 10 minutes after injecting 5 cc of the appropriate tumescent solution. The fat pad was trimmed and reimplanted to the interscapular area. After 3 months, fat pad was reharvested and sent for histopathologic evaluation. The harvested fat grafts were weighted in both surgical sessions. A significant difference was observed in comparison of fat grafts weights between the initial operation and the postoperative third month (p = 0.002). By intergroup comparisons, a significant difference was observed between sham and adrenaline groups (p = 0.002) and between sham and lidocaine + adrenaline groups (p = 0.007). No statistical difference was observed by the comparison of TUNNEL results (p = 0.663). The histopathologic evaluation of the specimens revealed similar results between groups. The injection of tumescent solutions containing only lidocaine before fat harvesting yields similar long-term fat graft survival rates in comparison to the conduction of surgical procedure without injecting any tumescent fluid. However, the injection of solutions containing adrenaline with or without lidocaine may decrease the long-term survival rates of fat autografts.

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Section: Discussionmentioning

confidence: 99%

Effects of Tumescent Solution on Fat Survival

et al. 2017

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“…Another study done by [17] concluded that maximal long-term durability and fat cell viability results were obtained in the groups with 2000 rpm or 447.2 G-force/4 minutes and 3000 rpm or 1006.2 Gforce/4 minutes centrifugation speed, indicating that 4 minutes centrifugation with an average G-force of 698.75 or 2500 rpm provides the best results for the survival of autogenous fat grafts.…”

Section: Improvementmentioning

confidence: 97%

Comparative Study between Platelet Rich Plasma and Adipose Derived Stem Cells in Periorbital Rejuvenation

Mikhael

Elhabaa

Shoulah

et al. 2020

Benha Journal of Applied Sciences

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Periorbital hyperpigmentation (POH) is a commonly encountered worldwide problem that is characterized by relatively dark coloration around the eyelids. Its difficulty is due to its resistance to treatment, complex pathogenesis, and lacking straight forward long-term therapeutic options. both PRP and adipose-derived stem cells are therapeutic options that could be used as an alternative form of therapy alone or in combination with other conventional treatments of POH. Research has to focus on standardizing the PRP and ADSCs formulations and have a consensus data from the clinical trials from different research groups for better prognosis and to use as an alternative to conventional therapies. In this study, we compare the effect of platelet rich plasma injection , depending on the fact that platelets secrete growth factors and cytokines that have a positive influence on tissue healing and regeneration, versus the effect of adipose derived stem cells , that release important growth factors necessary for wound healing, immune system modulation and reducing inflammation. Regarding the results of this study, Both fat injection and PRP are effective treatments of POH and were associated with improvement. Better response, patients' satisfaction was significantly associated with fat injection when compared with PRP. Patients' satisfaction and physician's evaluation were significantly associated with improvement. Both procedures were tolerated and accepted by the patients. Both modalities are promising and may represent a good temporary solution for the problem of POH.

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“…6,11,14,15 Historically, the majority of surgeons harvested fat with a syringe coupled with manual aspiration, 16 then performed centrifugation. However, since high-speed centrifugation is harmful to adipose tissue cells, 17–19 other techniques have been developed using decantation, filtration, or gentle centrifugations. 15,20–23 In addition, washing the tissue to remove potentially pro-inflammatory substances such as blood, oil, and cellular debris has been shown to improve outcomes.…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of a New Automatic System and Four Existing Techniques for Autologous Fat Grafting

Nelissen,

Licciardi,

Nizet

et al. 2023

Plastic and Reconstructive Surgery - Global Open

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Background: Autologous fat grafting is increasingly used worldwide and is a very attractive technique in many ways. However, treatment duration and postinjection tissue resorption remain problematic elements, which are largely related to the preparation method used. Moreover, few scientific studies objectively compare different fat preparation methods. This study analyzes the efficiency and quality of lipoaspirates prepared with a new filtration/centrifugation system (Adipure) in comparison with several existing techniques. Methods: Patient lipoaspirates were processed by five different techniques: decantation, centrifugation, Macrofill, Puregraft, and Adipure. Adipose tissue was evaluated in vitro for tissue resorption and oil formation, as well as in vivo after subcutaneous injections in immunodeficient mice. Adipose grafts were collected after 1 month, weighed, and analyzed by histology with a detailed scoring method. Results: Decanting gives inferior results to all other techniques, in terms of amount of tissue and oil in vitro, or graft weight and histological analysis in vivo. Methods using classical Coleman centrifugation (1200g), or a modified one (400g) associated with washes (Macrofill) produce very similar results, both in vitro and in vivo. Techniques using filtration systems (Puregraft and Adipure) produce less oil overall and have a higher grafting efficiency. The best results regarding grafting efficiency and oil quantity are found with the Adipure device. Conclusions: A combination of filtration and very low-speed centrifugation potentiates the advantages of these techniques, in terms of graft efficiency. The adipose tissue purification being done in a few minutes, in an automatic way, undoubtedly provides a strong advantage for the use of this new system.

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The effects of the centrifugation speed on the survival of autogenous fat grafts in a rat model

Cited by 10 publications

References 23 publications

Effects of Tumescent Solution on Fat Survival

Effects of Tumescent Solution on Fat Survival

Comparative Study between Platelet Rich Plasma and Adipose Derived Stem Cells in Periorbital Rejuvenation

Comparative Analysis of a New Automatic System and Four Existing Techniques for Autologous Fat Grafting

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