The Effects of Titanium and Hydroxyapatite on Osteoblastic Expression and Proliferation in Rat Parietal Bone Cultures

Massas, R.; Pitaru, S.; Weinreb, Max M.

doi:10.1177/00220345930720060201

Cited by 59 publications

(40 citation statements)

References 11 publications

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“…Massas et al 10 studied the effects of titanium, hydroxyapatite, and polystyrene (control) on the proliferation of rat calvarial cells and on the capacity to express alkaline phosphatase and to respond to parathyroid hormone stimulation. These authors reported that the nature of the substrate did not affect the DNA and protein contents of experimental and control cultures throughout the experimental period.…”

Section: Introductionmentioning

confidence: 99%

Porous bioactive glass and hydroxyapatite ceramic affect bone cell functionin vitro along different time lines

El‐Ghannam

Ducheyne

Shapiro

1997

J. Biomed. Mater. Res.

118

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We describe the effects on cell function of treating porous bioactive glass (BG) such that its surface is a composite of carbonated hydroxyapatite and serum protein. The effects on bone cell function of porous hydroxyapatite (HA) ceramic and porous glass treated to become amorphous calcium phosphate only also were studied subsequent to their having adsorbed a serum protein layer. Substrates treated for different durations were seeded with MC3T3-E1 cells and cultured for 3-17 days. Whereas cells seeded on any substrates, BG and HA produced collagen types I and III, bone sialoprotein, and osteopontin, there were significant differences between HA and BG, and among the various surface conditions created on BG. Covering the glass surface with hydroxyapatite and serum protein enhanced expression of high alkaline phosphatase activity, high rates of cell proliferation, and production of mineralized extracellular matrix. The enhancement may be due to the adsorption of a high quantity of fibronectin from the serum onto the reacted bioactive glass surface.

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Section: Introductionmentioning

confidence: 99%

Porous bioactive glass and hydroxyapatite ceramic affect bone cell functionin vitro along different time lines

El‐Ghannam

Ducheyne

Shapiro

1997

J. Biomed. Mater. Res.

118

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“…Osteoblasts cultured on HA-coated Ti showed high differentiation and mineralization compared with non-coated Ti 32,33) . Expression of ALP and parathyroid hormone response were higher in cultures grown on HA than in cultures grown on Ti 34) , and the in vitro formation of extracellular matrices was greater on CaP coatings than on Ti. Several mechanisms have been suggested to underlie osteogenesis on CaP ceramics.…”

Section: Discussionmentioning

confidence: 91%

Influence of thin carbonate-containing apatite coating with molecular precursor method to zirconia on osteoblast-like cell response

et al. 2014

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The influence of a thin carbonate-containing hydroxyapatite (CA) coating to tetragonal zirconia polycrystal (TZP) on osteoblastlike cell response was investigated. TZP disks were subjected to blasting and acid etching. Thin CA coatings were deposited by the molecular precursor method (TZP-CA). Initial cell adhesion of mouse osteoblast-like cells MC3T3-E1 was enhanced, and marked progress of actin filaments was observed on TZP-CA compared to on TZP. After 3, 5 or 7 days, cell proliferation on TZP-CA was significantly higher than that on TZP. Alkaline phosphatase activity was slightly lower on TZP-CA than on TZP at 7 days, and no difference was observed at 14 or 21 days. At 28 days incubation, collagenous fibers with mineral precipitants accompanied by phosphorous and amino groups were observed. These results indicate that thin CA coating with molecular precursor method offers promise as a means of enhancing cell response, particularly initial adhesion and proliferation of MC3T3-E1 cells.

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“…Alkaline phosphatase expression and parathyroid hormone response were higher in cultures grown in HA than in cultures grown in titanium, 12) and the in vitro formation of extracellular matrices was greater on Ca-P coatings than on titanium. Several mechanisms of the principal factors involved in osteogenesis on Ca-P ceramics have been considered.…”

Section: Surface Modifications Of Titanium With a Thin Cal-mentioning

confidence: 89%

Bio-Functionalization of Titanium Surfaces for Dental Implants

Yoshinari¹,

Matsuzaka

Inoue

et al. 2002

Mater. Trans.

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Since dental implants are used in contact with many different tissues, it is necessary to have optimum surface compatibility with the host bone tissues and soft tissues. Furthermore, dental implant surfaces exposed to the oral cavity must remain plaque-free. Such materials can be created under well-controlled conditions by modifying the surfaces of metals that contact those tissues. "Tissue-compatible implants," which are compatible with all host tissues, must integrate with bone tissue, easily form hemidesmosomes, and prevent bacterial adhesion. This research was aimed at developing such tissue-compatible implants by modifying titanium surfaces using a dry process for closely adhering to the titanium substrate and ensuring good wear resistance. The process includes ion beam dynamic mixing (thin calcium phosphates), ion implantation, titania spraying, ion plating and ion beam mixing. At the bone tissue/implant interface, a thin calcium phosphate coating and rapid heating with infrared radiation was effective in controlling the dissolution without cracking the coating. This thin calcium phosphate coating may directly promote osteogenisis, but also enable immobilization of functional proteins or drugs such as bisphosphonate for dug delivery system. At the oral fluid/implant interface, an alumina coating and F + -implantation were responsible for inhibiting the adhesion of microbial plaque. In conclusion, dry-process surface modification is useful in controlling the physicochemical nature of surfaces, including the surface energy and the surface electrical charge, and in developing tissue-compatible implants.

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The Effects of Titanium and Hydroxyapatite on Osteoblastic Expression and Proliferation in Rat Parietal Bone Cultures

Cited by 59 publications

References 11 publications

Porous bioactive glass and hydroxyapatite ceramic affect bone cell functionin vitro along different time lines

Porous bioactive glass and hydroxyapatite ceramic affect bone cell functionin vitro along different time lines

Influence of thin carbonate-containing apatite coating with molecular precursor method to zirconia on osteoblast-like cell response

Bio-Functionalization of Titanium Surfaces for Dental Implants

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