The effects of TPT and dietary quercetin on growth, hepatic oxidative damage and apoptosis in zebrafish

Zhang, Chunnuan; Jiang, Dongxue; Wang, Junhui; Qi, Qian

doi:10.1016/j.ecoenv.2021.112697

Cited by 21 publications

(9 citation statements)

References 59 publications

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“…Research has shown that adding baicalein to feed can reduce the serum MDA content of grass carp (Ctenopharyngodon idella) while increasing CAT and SOD activities, thereby enhancing antioxidant capacity [45]. Adding quercetin to feed can increase the antioxidant enzyme activity in the liver of Yellow River carp [46], zebrafish [47], common carp (Cyprinus carpio) [48], and snakehead (Channa argus) [49] and reduce liver MDA content. Adding an appropriate amount of Lycium barbarum Polysaccharide to feed can not only improve the growth performance and feed utilization of snakehead fish but also enhance its antioxidant capacity.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Short-Term Artificial Feed Domestication on the Expression of Oxidative-Stress-Related Genes and Antioxidant Capacity in the Liver and Gill Tissues of Mandarin Fish (Siniperca chuatsi)

Zhang,

Yuan,

et al. 2024

Genes

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To investigate whether Mandarin fish developed oxidative stress after being domesticated with artificial feed, we conducted a series of experiments. Oxidative stress is an important factor leading to diseases and aging in the body. The liver integrates functions such as digestion, metabolism, detoxification, coagulation, and immune regulation, while the gills are important respiratory organs that are sensitive to changes in the water environment. Therefore, we used the liver and gills of Mandarin fish as research materials. The aim of this study was to investigate the effects of short-term artificial feed domestication on the expression of oxidative stress genes and the changes in oxidative-stress-related enzyme activity in the liver and gills of Mandarin fish. We divided the Mandarin fish into two groups for treatment. The control group was fed with live bait continuously for 14 days, while the experimental group was fed with half artificial feed and half live bait from 0 to 7 days (T-7 d), followed by solely artificial feed from 7 to 14 days (T-14 d). The experimental results showed that there was no difference in the body weight, length, and standard growth rate of the Mandarin fish between the two groups of treatments; after two treatments, there were differences in the expression of genes related to oxidative stress in the gills (keap1, kappa, gsta, gstt1, gstk1, SOD, and CAT) and in the liver (GPx, keap1, kappa, gsta, gstt1, gr, and SOD). In the liver, GPx activity and the content of MDA were significantly upregulated after 7 days of domestication, while in the gills, SOD activity was significantly upregulated after 7 days of domestication and GPx activity was significantly downregulated after 14 days of domestication. These results suggest that artificial feed domestication is associated with oxidative stress. Moreover, these results provide experimental basic data for increasing the production of aquaculture feed for Mandarin fish.

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Section: Discussionmentioning

confidence: 99%

The Effect of Short-Term Artificial Feed Domestication on the Expression of Oxidative-Stress-Related Genes and Antioxidant Capacity in the Liver and Gill Tissues of Mandarin Fish (Siniperca chuatsi)

Zhang,

Yuan,

et al. 2024

Genes

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“…These results are consistent with previous studies [ 61 , 62 ]. Moreover, quercetin protects zebrafish livers against TPT-induced damage by increasing the activities of antioxidant enzymes, reducing ROS levels, inhibiting apoptosis, and by reducing inflammation [ 63 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Quercetin on the Intestinal Microflora of Freshwater Dark Sleeper Odontobutis potamophila

Zhu

Liu

et al. 2022

Antioxidants

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Flavonoids have antimicrobial and anti-oxidation properties. The effects of the flavonoid quercetin on the intestinal microflora of freshwater dark sleeper Odontobutis potamophila were tested for the first time. Odontobutis potamophila juveniles were treated with quercetin for 21 days at one of three concentrations (2.5, 5.0, or 10.0 mg/L) and compared with a control group that was not treated with quercetin. Quercetin improved the stability of the intestinal flora in O. potamophila and the probiotic bacteria Bacillus spp. and Lactobacillus spp. increased in species abundance after the low concentration quercetin treatments. Furthermore, the abundance of pathogenic bacteria Plesiomonas spp., Aeromonas spp., and Shewanella spp. decreased after the fish had been exposed to quercetin. Activity of hepatic antioxidant enzymes (superoxide dismutase, SOD), (glutathione S-transferase, GST), (glutathione peroxidase, GSH-Px), and (total antioxidant capacity, T-AOC) increased in the livers of O. potamophila treated with quercetin, thereby increasing their hepatic antioxidant capacity and their ability to scavenge free radicals.

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“…TPT disrupts lipid metabolism in Marisa cornuarietis at environmental concentrations 9 . TPT also interferes with retinal axon development in fish at low doses 10 , induces oxidative stress and apoptosis in zebrafish 11 , and disrupts glucocorticoid synthesis in the rat adrenal cortex via several mechanisms: 1) it lowers serine/threonine kinase 1 (AKT1) phosphorylation and Sirtuin 1 (SIRT1)/peroxisome proliferator-activated receptor-gamma coactivator 1α (PGC-1α) levels, 2) activates AMP-activated protein kinase (AMPK), and 3) possibly induces reactive oxidative species (ROS) production 12 . TPT also appears to affect reproduction and mortality at decreasing concentrations in temporally overlapping generations 13 .…”

Section: Introductionmentioning

confidence: 99%

Short-term triphenyltin exposure alters microbial homeostasis in the silkworm (Bombyx mori) midgut

Zhou,

Zhang,

Chen

et al. 2023

Sci Rep

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Triphenyltin (TPT) is a widespread synthetic chemical used in many fields and its potential risk to organisms has been comprehensively investigated using different animal models and species. Currently, little is known about the effects of TPT exposure on microbial midgut diversity, therefore we explored these effects in the lepidopterous silkworm model using 16S rDNA sequencing. In total, 5273 and 5065 operational taxonomic units (OTUs) were identified in control and TPT-exposure group samples, ranging from 424 to 728 OTUs/sample. Alpha-diversity analyses revealed that TPT exposure induced the fluctuations of gut microbial diversity and abundance while beta-diversity analyses identified a distinct impact on major gut microbiota components. In our microbiome analyses, 23 phyla and 353 genera were recognized in the control group, while 20 phyla and 358 genera were recognized in the TPT exposure group. At the genus level, midgut microbiota were composed of several predominant bacterial genera, including Muribaculaceae, Lactobacillus, and UCG-010. In the TPT exposure group, o__Bacillales, f__Bacillaceae, and f__Caldicoprobacteraceae abundance was relatively high, while f__Oscillospiraceae, f__Fusobacteriaceae, and f__SC_I_84 abundance was relatively high in the control group. Gene function analyses in silkworm microbiota after TPT exposure showed that biosynthesis of ansamycins, fructose and mannose metabolism, glycerolipid metabolism, type II diabetes mellitus, glycolysis/gluconeogenesis, lipid metabolism, translation proteins, atrazine degradation, DNA repair and recombination proteins, nicotinate and nicotinamide metabolism were significantly increased. Collectively, our silkworm model identified gut microbial diversity risks and the adverse effects from TPT exposure, which were similar to other aquatic animals. Therefore, TPT levels in environmental samples must be monitored to prevent ecological harm.

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The effects of TPT and dietary quercetin on growth, hepatic oxidative damage and apoptosis in zebrafish

Cited by 21 publications

References 59 publications

The Effect of Short-Term Artificial Feed Domestication on the Expression of Oxidative-Stress-Related Genes and Antioxidant Capacity in the Liver and Gill Tissues of Mandarin Fish (Siniperca chuatsi)

The Effect of Short-Term Artificial Feed Domestication on the Expression of Oxidative-Stress-Related Genes and Antioxidant Capacity in the Liver and Gill Tissues of Mandarin Fish (Siniperca chuatsi)

Effects of Quercetin on the Intestinal Microflora of Freshwater Dark Sleeper Odontobutis potamophila

Short-term triphenyltin exposure alters microbial homeostasis in the silkworm (Bombyx mori) midgut

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