The effects of turbulent jet flows on plant cell suspension cultures

MacLoughlin, P. F.; Malone, Dermot M.; Murtagh, J. T.; Kieran, Patricia

doi:10.1002/(sici)1097-0290(19980620)58:6<595::aid-bit5>3.0.co;2-d

Cited by 29 publications

(8 citation statements)

References 29 publications

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“…Thus, plant cells are more susceptible to shear stress during the late exponential growth and early stationary phases when the cells are of relatively large size and contain large vacuoles [126]. Numerous studies on the shear sensitivity of cultured plant cells to hydrodynamic environment have been comprehensively investigated (detailed in [80,121,122,[128][129][130][131][132]) by either cultivating suspended cells under shear forces (regulated by changes of agitation speed and/or aeration rate) during the period of cultivation in bioreactors or exposing plant cells to well-defined, laminar or turbulent flow conditions in specific devices. However, the clear mechanism of hydrodynamic shear stress induced-damage to suspended plant cells is not well understood due to the diversity of cell lines, aggregate size distribution and cell morphologies, cell wall composition and culture age.…”

Section: Shear Sensitivity Of Suspended Plant Cell Culturesmentioning

confidence: 99%

Bioreactor engineering for recombinant protein production in plant cell suspension cultures

Huang

McDonald

2009

Biochemical Engineering Journal

162

105

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Section: Shear Sensitivity Of Suspended Plant Cell Culturesmentioning

confidence: 99%

Bioreactor engineering for recombinant protein production in plant cell suspension cultures

Huang

McDonald

2009

Biochemical Engineering Journal

162

105

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“…A limitation associated with this approach is that different threshold values may be obtained depending on the duration of exposure of the cells to the shear environment. In general, shear effects have been examined in either short‐term (0.5 s to 12 h) experiments under well‐defined conditions of laminar or turbulent flow (Hooker et al, 1989; Kieran et al, 1995; MacLoughlin et al, 1998; Zhong et al, 1994), or in short‐ or long‐term experiments in bioreactors under relatively ill‐defined hydrodynamic conditions (Leckie et al, 1991b; Markx et al, 1991; Scragg et al, 1988a; Takeda et al, 1994; Zhong et al, 1994).…”

Section: Analysis Of Shear Effectsmentioning

confidence: 99%

Design of Mixing Systems for Plant Cell Suspensions in Stirred Reactors

Doran

1999

Biotechnol. Prog.

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The properties of dense plant cell suspensions, such as high viscosity, shear sensitivity, cell aggregation, and tendency to foam, present significant technical challenges for bioreactor design and operation. As in all fermentation processes, reactors used for large-scale plant cell culture must provide adequate mixing and mass transfer at nondamaging levels of hydrodynamic shear. In stirred vessels, this means that the impeller must be operated with restricted power input and speed, while still achieving complete gas dispersion and solids suspension. Although many experimental studies of conventional and novel impellers have been carried out, no clear guidelines for impeller choice have emerged. In this paper, an engineering analysis is used to identify which agitators and operating regimes have greatest potential for high-density plant culture applications, irrespective of whether they have been tested experimentally. Ways of improving impeller performance by modifying internal reactor geometry are also discussed.

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“…Programmed death was observed as a defense mechanism involving DNA cleavage upon pathogens infection (Mittler et al, 1996;Ryerson et al, 1996). Necrosis, on the other hand, is a passive phenomenon resulting from dramatic stresses in the cell environment (Dunlop et al, 1994;Kieran et al, 1995;MacLoughlin et al, 1998;Takeda et al, 1998). The consequence is a disappearance of cells from the tissue or the culture medium.…”

Section: Introductionmentioning

confidence: 99%

Determination of growth and lysis kinetics in plant cell suspension cultures from the measurement of esterase release

Steward

Martin

Engasser

et al. 1999

Biotechnol. Bioeng.

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The death of Medicago sativa L. cells cultivated in a batch culture was investigated by measuring both the appearance of intact dead cells determined on the basis of the trypan blue (TB) dye exclusion, and the release of the cytoplasmic esterase activity into the culture medium upon cell death. Taking into account the strong instability of this released esterase activity, the total dead cell and lysed cell densities have been estimated. A mechanism for cell death and lysis is proposed and the specific rates of cell growth, death and lysis estimated. The specific rate of appearance of TB dead cells was low and essentially constant (0.25 day−1) during the first 8 days of the batch culture, and then increased above 1.5 day−1 after 2 weeks of cultivation. Whereas no lysis occurred during the first seven days, this phenomenon occurred during the second period and accounted for about 20% of the total cell death by the end of the process. Thus, the viability determined by the trypan blue exclusion method appeared to be invalid after 7 days of culture. When lysis of viable cells is taken into consideration, the specific growth rate was significantly increased and growth was shown to continue for a further 8 days. Increased sensitivity of the cells to shear stresses and consequent cell lysis could be the result of a 35% increase in the cell size © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 66: 114–121, 1999.

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The effects of turbulent jet flows on plant cell suspension cultures

Cited by 29 publications

References 29 publications

Bioreactor engineering for recombinant protein production in plant cell suspension cultures

Bioreactor engineering for recombinant protein production in plant cell suspension cultures

Design of Mixing Systems for Plant Cell Suspensions in Stirred Reactors

Determination of growth and lysis kinetics in plant cell suspension cultures from the measurement of esterase release

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