The effects of type 1 diabetes mellitus and of tobacco smoke on dissipation of catecholamines in pulmonary endothelial cells — a non-neuronal site of uptake1

Bryan-Lluka, Lesley J.; McKee, G. J.

doi:10.1007/978-3-7091-9175-0_4

Cited by 1 publication

(1 citation statement)

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“…Changes in COMT in the kidney need to be characterized because combined anti‐oxidant and COMT inhibitor treatment has been reported to reverse renal abnormalities in diabetic rats 14 . Otherwise, monoamine clearance has been documented to be increased in the lungs of type I diabetics 15 . Moreover, it shall also be concerned that the decrease of COMT activity is produced in type I diabetic disorder only.…”

Section: Discussionmentioning

confidence: 99%

Decrease In Catechol‐O‐Methyltransferase Activity In The Liver Of Streptozotocin‐Induced Diabetic Rats

Wang

Liu

Tzeng

et al. 2002

Clin Exp Pharma Physio

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1. The present study compared the activity of catechol- O-methyltransferase (COMT) in the liver and plasma of streptozotocin (STZ)-induced diabetic rats with that in normal rats. The activity of COMT was estimated by the metabolism of noradrenaline to metanephrine (MN), both measured by high-performance liquid chromatography with electrochemical detection. 2. Formation of MN was decreased in the liver of STZ- diabetic rats compared with normal rats. The amount of MN was also decreased in plasma obtained from STZ-diabetic rats. A Michaelis-Menten plot showed a reduction in the maximum velocity and an increase in the Km for COMT in liver samples from STZ-diabetic rats. 3. The role of hyperglycaemia in the lowering of COMT activity was then investigated using phlorizin or insulin at doses sufficient to normalize plasma glucose from STZ-diabetic rats. Both insulin and phlorizin treatment of STZ-diabetic rats for 4 days restored the activity of COMT to that seen in normal rats. Thus, correction of hyperglycaemia in STZ-diabetic rats can reverse the decrease in COMT activity. The activity of COMT is lower in STZ-diabetic rats than in normal rats mainly due to the higher plasma glucose.

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Section: Discussionmentioning

confidence: 99%