“…thermal, chemical or other mechanical methods, ultrasound (US) has gained interest for such purpose, as it provides efficient sludge disintegration (Pilli et al, 2011;Tyagi et al, 2014) and does not require any chemical additive. Ultrasonic pretreatment was reported to improve biodegradability and bio-solid quality (Khanal et al, 2007;Trzcinski et al, 2015), to enhance biogas/methane production (Barber, 2005;Braguglia et al, 2015;Khanal et al, 2007;Onyeche et al, 2002), to reduce excess sludge (Onyeche et al, 2002) and required sludge retention time (Tiehm et al, 1997).…”