The effects of various antioxidants on the development of parthenogenetic porcine embryos

Yuh, Hyung Soo; Yu, Dong; Shin, Mi Jung; Kim, Hei Jung; Bae, Ki Beom; Lee, Dong Seok; Lee, Hwi Cheul; Chang, Won-Kyong; Park, Soo Bong; Kim, Tae Hyun; Park, Hum Dai; Ha, Jung‐Hong; Hyun, Byung Hwa; Ryoo, Zae Young

doi:10.1007/s11626-009-9250-1

Cited by 11 publications

(6 citation statements)

References 30 publications

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“…Recent studies have indicated the effects of antioxidants on the development of parthenogenetic porcine embryos. Antioxidants increase the average number of total cells at the blastocyst [55]. However, in this study no difference was detected between the P. bracteatum and the control group in the rate and developmental competence of oocytes obtained through IVF.…”

Section: Discussioncontrasting

confidence: 79%

The effect of intra‐peritoneal administration of Papaver bracteatum Lindl. extract on development of NMRI mice oocytes treated with Doxorubicin

Jafarian

Eimani

Azarnia

et al. 2012

Reprod Medicine & Biology

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Purpose The effect of water-alcohol Papaver bracteatum Lindl. extract on development of mice oocytes treated with Doxorubicin (dox) was examined in this study. Methods The mice were classified into four groups. Control group, mice injected intraperitoneally (IP) with saline. Extract group alone, mice treated with 200 mg/kg of body weight (bw), IP, twelve consecutive days. Dox group alone, mice were given dox, IP, 10 mg/kg bw. Experimental group treated with extract and dox together. Effect of the extract on the development of mice oocytes treated with dox were evaluated through assisted reproductive technology techniques (ARTs). Results Developmental rate and blastocyst formation was improved by using the extract. A significant increase in in vitro developmental competence in comparison with dox group (P \ 0.05) was observed. Conclusions The results of this study indicated that P. bracteatum Lindl. extract could prevent dox toxicity of dox affecting both follicle or oocytes, and therefore it can result in improved embryo development which was observed in mice treated with dox plus P. bracteatum Lindl. compared to mice treated with dox alone.

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Section: Discussioncontrasting

confidence: 79%

The effect of intra‐peritoneal administration of Papaver bracteatum Lindl. extract on development of NMRI mice oocytes treated with Doxorubicin

Jafarian

Eimani

Azarnia

et al. 2012

Reprod Medicine & Biology

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“…Treatment with pGM-CSF in the IVC medium also improved the developmental potential of porcine IVF embryos (28). In addition, the supplementation of IVC medium with β-ME was able to increase the developmental competence of porcine PA embryos (29). β-ME was also able to increase intracellular glutathione levels and reduce damage to the embryo caused by ROS, thus improving embryo development.…”

Section: Discussionmentioning

confidence: 88%

Improvement in the blastocyst quality and efficiency of putative embryonic stem cell line derivation from porcine embryos produced in vitro using a novel culturing system

Cheong

Kim

Kwak

et al. 2015

Molecular Medicine Reports

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Porcine embryonic stem cells (pESCs) have great potential for application in translational biomedical research, including xenotransplantation and disease models. Obtaining high-quality blastocysts is the most important factor in the isolation and colonization of primary ESCs and the establishment of ESC lines. In pigs, in vitro-derived blastocysts have a limited cell number compared to in vivo-derived blastocysts and show an indefinite inner cell mass, which may result in failure to establish pESC lines. In the present study, the effects of resveratrol (RES), granulocyte-macrophage colony stimulating factor (GM-CSF) and β-mercaptoethanol (β-ME) on the quality of blastocysts and the efficiency of colony derivation were investigated for the establishment of ESCs. A novel culturing system was developed in which 2 µM RES was added to the oocyte in vitro maturation (IVM) medium, and 10 ng/ml pGM-CSF and 10 µM β-ME were added to embryo in vitro culture (IVC) medium. This novel system showed significantly more parthenogenetic activation (PA) blastocysts (54.5 ± 1.8% vs. 43.4 ± 1.2%; P<0.05) and in vitro fertilization (IVF) blastocysts (36.9 ± 3.3% vs. 26.2 ± 2.9%; P<0.06) at day seven as compared with that in the control system. The PA and IVF blastocysts from the novel system showed a significantly greater hatching rate (P<0.05) and greater cell numbers (55.1 ± 2.0 vs. 45.6 ± 2.0; P<0.05 and 78.9 ± 6.8 vs. 58.5 ± 7.2; P<0.06, for PA and IVF, respectively) at day seven compared to that in the control system. After seeding on feeder cells, the PA blastocysts produced by the novel system showed a significantly increased rate of attachment (28.8 ± 3.9% vs. 17.2 ± 2.4%; P<0.062). Finally, two putative pESC lines from PA embryos produced by the novel system and one by the control system were established. In conclusion, the novel system improved blastocyst quality and increased the derivation efficiency of putative pESC lines from porcine PA and IVF embryos produced in vitro.

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“…Usually, supplementation of embryo culture media with antioxidants is associated not only with improvements in embryo production rates, but also with enhancing embryo quality 12 , 33 . In our study, the increased blastocyst rates observed after supplementation with 10 µM NMPG were not associated with an improvement in embryonic quality in terms of TCN or the ICM/TE ratio.…”

Section: Discussionmentioning

confidence: 99%

N-(2-mercaptopropionyl)-glycine enhances in vitro pig embryo production and reduces oxidative stress

Cambra

Martínez

Rodriguez‐Martinez

et al. 2020

Sci Rep

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This study evaluated the effects of different concentrations (1, 10, 25, 50, and 100 µM) of the antioxidant N-(2-mercaptopropionyl)-glycine (NMPG), during the culture of in vitro-fertilized porcine oocytes. While the highest concentrations of NMPG (50 and 100 µM) were toxic to the developing embryos during the first two days of culture, 25 µM NMPG achieved cleavage rates that were similar to those achieved by the control but did not sustain blastocyst production by Day 7 of culture. Compared to the control culture medium, the culture medium supplemented with 10 µM NMPG increased (P < 0.05) the rates of blastocyst formation, decreased (P < 0.05) the intracellular levels of reactive oxygen substances, and downregulated (P < 0.05) the expression of the oxidative stress related gene GPX1. In conclusion, these results demonstrated that supplementation of porcine embryo culture medium with 10 µM NMPG can attenuate oxidative stress and increase the yield of in vitro production of blastocysts.

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The effects of various antioxidants on the development of parthenogenetic porcine embryos

Cited by 11 publications

References 30 publications

The effect of intra‐peritoneal administration of Papaver bracteatum Lindl. extract on development of NMRI mice oocytes treated with Doxorubicin

The effect of intra‐peritoneal administration of Papaver bracteatum Lindl. extract on development of NMRI mice oocytes treated with Doxorubicin

Improvement in the blastocyst quality and efficiency of putative embryonic stem cell line derivation from porcine embryos produced in vitro using a novel culturing system

N-(2-mercaptopropionyl)-glycine enhances in vitro pig embryo production and reduces oxidative stress

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