The effects of vasopressin and related peptides on osmoregulation in Amoeba proteus

Couillard, Pierre; Pothier, F.; Mayers, Philippe

doi:10.1016/0016-6480(89)90037-3

Cited by 11 publications

(3 citation statements)

References 26 publications

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“…The contractile vacuole is found in close association with the Golgi apparatus [42]. The concept of volume and osmotic regulation is well established in the case of the contractile vacuoles of protozoa and algae [14][15][16]. Water exclusion into the…”

Section: Discussionmentioning

confidence: 99%

“…Radiation-induced vacuoles in 3T3 cells are also derived from the endoplasmic reticulum and Golgi apparatus [17], but ceramide induces numerous vesicles of lysosome without toxic effects [41]. In many types of amoeba, numerous vesicles in small diameter as well as tubular structures derived from endoplasmic reticulum are believed to exude their contents into the contractile vacuole through fusion [14][15][16]. The contractile vacuole is found in close association with the Golgi apparatus [42].…”

Section: Discussionmentioning

confidence: 99%

“…Contractile vacuoles are present in amoeba and contribute to the regulation of water content [14][15][16]. Vacuoles in cells are formed by radiation injury, virustransformation and toxin injury [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

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External Cl<sup>-</sup>-Dependent Formation of Watery Vacuoles by Long-Term Hypotonic Shock in 3T3-L1 Cells

Iwasa

Hirono²,

Sugita³

et al. 2001

Cell Physiol Biochem

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Osmotic shock transiently induces a volume change in the cells, followed by a restoration of the cell volume due to intracellular water regulation. Effect of long-term osmotic shock on the water regulation is not completely understood. Vacuole formation by long-term osmotic shock was investigated to clarify the water exclusion mechanism from cytoplasm into intracellular vacuoles in 3T3-L1 cells. Incubation of cells in hypotonic solution reversibly induced the vacuole formation. Staining of vacuoles with fluorescent dyes revealed that vacuoles were derived from endoplasmic reticulum and Golgi apparatus but not lysosomes. Membrane-impermeable fluorescent dyes were taken up into some vacuoles from cytoplasm and extracellular solution, suggesting that some vacuoles exhibit the dynamic changes for the connection of plasma membrane, and that transporter for membrane-impermeable dyes might be active in some vacuole membranes. External Cl^-, but not Na⁺, was required for vacuole formation. DPC suppressed the vacuole formation and increased cell height, and further incubation with DPC increased the number of dead cells. Bumetanide, dimethylamiloride, and HgCl₂ did not suppress the hypotonic stress-induced formation of water vacuoles. These findings suggest that 3T3-L1 cells regulate the intracellular water content through the DPC-sensitive external Cl^--dependent vacuole formation during long-term osmotic stress.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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External Cl<sup>-</sup>-Dependent Formation of Watery Vacuoles by Long-Term Hypotonic Shock in 3T3-L1 Cells

Iwasa

Hirono²,

Sugita³

et al. 2001

Cell Physiol Biochem

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Vasopressin‐immunoreactive neurons and neurohemal systems in cockroaches and mantids

Davis

Hildebrand

1992

J of Comparative Neurology

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Vasopressin-like neuropeptides of insects are of special interest because of their possible function as hormones and neuromodulators. Therefore, this study was undertaken by using whole-mount immunofluorescent staining by two antisera that recognize different types of vasopressin-like immunoreactive groups of neurons in the cockroaches Periplaneta americana, Leucophaea maderae, Nauphoeta cinerea, Diploptera punctata, and Blaberus discoidalis and in the mantids Litaneuria minor and Tenodera aridifolia sinensis. Using an antiserum to Arg/vasopressin, only two cells, the paired ventral paramedian (PVP) neurons, were immunostained in the central nervous system (CNS) of the cockroaches. These cells are located in the subesophageal ganglion, project throughout the CNS, and appear to be neurosecretory. Their varicose collaterals extend into the dorsal (motor) neuropil of the segmental ganglia, and this neuropil may be the principal site of the release of their neurosecretion. The PVP neurons were also stained by an antiserum to Lys/vasopressin; in addition, this antiserum stained several other groups of neurons, most of which appeared to be neurosecretory. Two pairs of Lys/vasopressin-immunoreactive cells are similar to the PVP neurons in that they are located in the subesophageal ganglion, extend through the ventral nerve cord, have collaterals in the dorsal neuropil of the segmental ganglia, and appear to be neurosecretory within the CNS. In addition, midventral and anteroventral clusters of Lys/vasopressin-immunoreactive neurosecretory neurons in the subesophageal ganglion project neurohemal release sites on the corpora allata. Other types of Lys/vasopressin-immunoreactive neurons include median and lateral neurosecretory cells of the protocerebrum and neurosecretory cells in the tritocerebrum, all of which project to the corpora cardiaca. In the abdominal ganglia there are posterolateral clusters of Lys/vasopressin neurosecretory neurons, and these cells extend to neurohemal release sites on the transverse and lateral cardiac nerves. In mantids the anti-Arg/vasopressin and anti-Lys/vasopressin antisera stained most of the same groups of neurons that these antisera recognized in cockroaches. The results of this study suggest that there are two or more vasopressin-like peptides in cockroaches and mantids and that these peptides may be released either as hormones in the blood or as neurosecretions within the CNS. Their function(s) in these insects remains to be determined.

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Peculiarities of Signal Transduction from Arginine-Vasopressin Receptors Located on the Apical Surface of Epithelial Cells of the Frog Urinary Bladder and on the Amoeba Outer Membrane

Bagrov

Manusova

2005

J Evol Biochem Phys

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The effects of vasopressin and related peptides on osmoregulation in Amoeba proteus

Cited by 11 publications

References 26 publications

External Cl<sup>-</sup>-Dependent Formation of Watery Vacuoles by Long-Term Hypotonic Shock in 3T3-L1 Cells

External Cl<sup>-</sup>-Dependent Formation of Watery Vacuoles by Long-Term Hypotonic Shock in 3T3-L1 Cells

Vasopressin‐immunoreactive neurons and neurohemal systems in cockroaches and mantids

Peculiarities of Signal Transduction from Arginine-Vasopressin Receptors Located on the Apical Surface of Epithelial Cells of the Frog Urinary Bladder and on the Amoeba Outer Membrane

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