The effects of vitrification on gene expression in mature mouse oocytes by nested quantitative PCR

Habibi, Anoosha; Farrokhi, Naser; Silva, Fernando Moreira da; Bettencourt, Bruno Filipe; Bruges-Armas, Jácome; Amidi, Fardin; Hosseini, Ahmad

doi:10.1007/s10815-010-9453-0

Cited by 26 publications

(17 citation statements)

References 23 publications

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“…It has been known that cryopreservation could modulate several internal genes within oocytes (24). In the oocyte, spindle checkpoint proteins play a role of controlling entry into anaphase and chromosome segregation.…”

Section: Genementioning

confidence: 99%

Effect of antifreeze protein supplementation in vitrification medium on mouse oocyte developmental competence

Jee

Lee

et al. 2011

Fertility and Sterility

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Section: Genementioning

confidence: 99%

Effect of antifreeze protein supplementation in vitrification medium on mouse oocyte developmental competence

Jee

Lee

et al. 2011

Fertility and Sterility

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“…Here, in a continuation of our previous studies (Habibi et al, 2010(Habibi et al, , 2011, a lower concentration of CPAs (7.5% EG and 7.5% DMSO) next to the higher concentration reported earlier by Kuwayama and Kato (2000) (15% EG and 15% DMSO) was applied to vitrify four-cell embryos and compared with non-vitrified cells at the same developmental stage both at the molecular level and morphological features. In comparison with blastocysts, four-cell mouse embryos have smaller volume and therefore one can accommodate more on vitrification tools such as Cryotop and further to this note it requires shorter period of time to collect them.…”

mentioning

confidence: 82%

“…Amongst a variety of exploratory molecular techniques, transcript analysis, gene-based or global analysis, provide some insights in the early development (Di Pietro et al, 2010;Habibi et al, 2010), better understanding of how to treat the cells to minimize the probable side-effects of vitrification and somewhat demonstrates the well-being of the cells during and subsequent to the cryopreservation (Mamo et al, 2006). For example, transcript analysis of histone deacetyltransferase 1 expression in mouse demonstrated a reduced expression upon cryopreservation indicating a probable negative impact on embryo development (Li et al, 2011).…”

mentioning

confidence: 99%

Transcript analysis of Heat shock protein 72 and protein 53 of 4-cell mouse embryos following Cryotop vitrification

Habibi¹,

Farrokhi²,

Silva³

et al. 2013

CZECH J ANIM SCI

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ABSTRACT:The effects of two different concentrations of cryoprotectants on survival and developmental capacity of four-cell mouse embryos were compared by Cryotop vitrification to demonstrate that lower concentrations provide the same results as higher previously reported concentrations with lesser negative molecular impact on embryo cells. For this latest, embryos were compared via transcript analyses of Heat shock protein 72 (Hsp72) and protein 53 (p53). Four-cell embryos were obtained from superovulated female mice and randomly assigned to one of three following groups: (i) control (non-vitrified), (ii) vit 1 (15% v/v: 7.5% ethylene glycol (EG) and 7.5% dimethyl sulfoxide (DMSO), and (iii) vit 2 (30% v/v: 15% EG + 15% DMSO). The cells vitrified by Cryotop were thawed and side-by-side to the control group divided into two parts: one part was used to analyze the morphological traits, survival rate, and embryo cleavage ability to form blastocysts, and the other part was examined for changes in transcript levels of Hsp72 (Hspa1a + Hspa1b), p53, and Hprt1 (reference gene) by quantitative Real-Time polymerase chain reaction (qPCR). The results were analyzed by One Way Analysis of Variance and the mean values compared with LSD (P < 0.05). The relative expression of p53 in vit 2 (30% v/v) was significantly higher than in vit 1 (15% v/v) and in vit 1 it was higher than in the control. The relative expression of Hsp72 was the same in vit 1 and vit 2 and significantly higher than in the control. The survival, cleavage, and blastocyst rates were the same for both vitrification treatments and significantly lower than in the control group. The up-regulations of Hsp72 and p53 following vitrification were suggestive of imposed heat shock, cold stress, and DNA damage to the mouse 4-cell embryos.

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“…The output from the real-time PCR assays were C T values that represent the PCR cycle at which the amplification crosses a given threshold (0.1). All C T values in Table S4; data are plotted and analyzed as inverse C T representing the relevant abundance 66,80 assuming equal between species in the consortia.…”

Section: Methodsmentioning

confidence: 99%

Lactobacillus acidophilusdisrupts collaborative multispecies bile acid metabolism

Bernstein

Dautel

Khan

et al. 2018

Preprint

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26Bile acids are metabolic links between hosts and their gut microbiomes, yet little is known about 27 the roles they play in microbe-to-microbe interactions. Here we present a study designed to 28 investigate the effect that a common probiotic, Lactobacillus acidophilus, has on microbial 29interactions that lead to formation of secondary bile acids. A model microbial consortium was 30 built from three human gut isolates, Clostridium scindens, Collinsella aerofaciens, and Blautia 31 obeum, and cultured under different bile acid and probiotic treatments. A multi-omics platform 32 that included mass spectrometry-based metabolomics and activity-based proteomic probes was 33 used to produce two major results. The first, was that an uncommon secondary bile acid -34 ursocholate -was produced by a multi-species chemical synthesis pathway. This result 35 highlights a new microbe-to-microbe interaction mediated by bile acids. The second finding was 36 that the probiotic strain, L. acidophilus, quenched the observed interactions and effectively 37 halted consortial synthesis of ursocholate. Little is known about the role that ursocholate plays in 38 human health and development. However, we did discover that a decrease in ursocholate 39 abundance corresponded with successful weight loss in patients after gastric bypass surgery 40 versus those who did not lose weight after surgery. Hence, this study uncovered basic knowledge 41 that may aid future designs of custom probiotic therapies to combat obesity. 42 43 44 Probiotic supplementation likely reduces the risk of developing antibiotic associated diarrhea 9 53 and necrotizing enterocolitis in infants 10 . However, the therapeutic opportunities for probiotics 54 are advancing to more precisely target specific processes carried out by the gut microbiome to 55 impart health benefits beyond enhanced digestion [11][12][13] . Probiotic therapies are being explored 56 relieve symptoms of autism 14 , depression 4 , autoimmune diseases 15,16 , and irritable bowel 57 syndrome 17 among many other conditions with positive -albeit conflicting -results. The 58 efficacy of probiotic treatments is variable. Differing results can obviously arise from 59 inconsistent study design -e.g., probiotic strain, dose -trial size, but they are also indicative of a 60 large scientific knowledge gap and incomplete understanding of the mechanisms by which 61 probiotics impact the GI-tract microbial ecosystem. 62There are many hypotheses about the modes of action by which the gut microbiome and 63 probiotic microbes impact human health. One proposed model is through modulation of the host 64 immune system 18 , which has been concluded from studies that showed probiotic treatments 65 affecting host immune function in humans facing pathogenic challenges [19][20][21] or with autoimmune 66 disorder 15,16,22 . Another hypothesis is that probiotics increase microbial competition within the 67 4 intestinal ecosystem, thereby making it more difficult for pathogenic bacteria to survive 23,24 . 68 Studies ...

show abstract

The effects of vitrification on gene expression in mature mouse oocytes by nested quantitative PCR

Abstract: although vit(1) treatment had lower survival rate compared to control group, it demonstrated better stability comparing to vit(2) based on the transcript analysis.

Cited by 26 publications

References 23 publications

Effect of antifreeze protein supplementation in vitrification medium on mouse oocyte developmental competence

Effect of antifreeze protein supplementation in vitrification medium on mouse oocyte developmental competence

Transcript analysis of Heat shock protein 72 and protein 53 of 4-cell mouse embryos following Cryotop vitrification

Lactobacillus acidophilusdisrupts collaborative multispecies bile acid metabolism

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