The effects of wumei pill on intestinal flora and neurotransmitters in rats with diarrhea-predominant irritable bowel syndrome (IBS-D)

Ding, Xiaojie; Sun, Xiling; Yu, Xiaofei; Zhao, Shutian; Yang, Yi; Xu, Wenjuan; Wang, Kejun; Zou, Xiaolan; Dong, Zhao

doi:10.1063/1.5092406

Cited by 1 publication

(1 citation statement)

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“…It exerts beneficial effects through its primary active component, polysaccharides, which has been reported to help treat diarrhea [ 13 ]. Another famous Chinese herbal formula, Wumei Pill, is useful for treating diarrhea-predominant irritable bowel syndrome (IBS-D) by improving the ratio of Bifidobacterium/Enterobacteriaceae (B/E value) and reducing the hippocampal tissue glutamate (Glu), Î³-aminobutyric acid (Î³-GABA), dopamine (DA) and 5-hydroxytryptamine (5-HT) contents [ 14 ]. Through more than two decades of clinical practice and research, we created a novel Chinese herb, called ‘modified Renshen Wumei decoction (MRWD),’ which could be an external therapeutic agent to help ameliorate symptoms of persistent diarrhea.…”

Section: Introductionmentioning

confidence: 99%

Modified Renshen Wumei Decoction Alleviates Intestinal Barrier Destruction in Rats with Diarrhea

Guan

Zhao

Huang

et al. 2021

J. Microbiol. Biotechnol.

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Modified Renshen Wumei decoction (MRWD), a famous traditional Chinese medicine, is widely used for treating persistent diarrhea. However, as the mechanism by which MRWD regulates diarrhea remains unknown, we examined the protective effects of MRWD on intestinal barrier integrity in a diarrhea model. In total, 48 male rats were randomly distributed to four treatment groups: the blank group (CK group), model group (MC group), Medilac-Vita group (MV group) and Chinese herb group (MRWD group). After a 21-day experiment, serum and colon samples were assessed. The diarrhea index, pathological examination findings and change in D-lactate and diamine oxidase (DAO) contents illustrated that the induction of diarrhea caused intestinal injury, which was ameliorated by MV and MRWD infusion. Metabolomics analysis identified several metabolites in the serum. Some critical metabolites, such as phosphoric acid, taurine, cortisone, leukotriene B4 and calcitriol, were found to be significantly elevated by MRWD infusion. Importantly, these differences correlated with mineral absorption and metabolism and peroxisome proliferator-activated receptor (PPAR) pathways. Moreover, it significantly increased the expression levels of TLR4, MyD88 and p-NF-κB p65 proteins and the contents of IL-1 and TNF-α, while the expression levels of occludin, claudin-1 and ZO-1 proteins decreased. These deleterious effects were significantly alleviated by MV and MRWD infusion. Our findings indicate that MRWD infusion helps alleviate diarrhea, possibly by maintaining electrolyte homeostasis, improving the intestinal barrier integrity, and inhibiting the TLR4/NF-κB axis.

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Section: Introductionmentioning

confidence: 99%