The effects of α-zearalanol on the proliferation of bone-marrow-derived mesenchymal stem cells and their differentiation into osteoblasts

Abstract

The aim of this study was to explore the effects of α-zearalanol (α-ZAL) on the proliferation of mouse bone-marrow-derived mesenchymal stem cells (BMSCs) and their differentiation into osteoblasts. Six- to eight-week-old BALB/C mice were used either as recipients or as bone marrow donors. BMSCs were isolated and collected using a differential adhesion method, with use of 10 % fetal bovine serum and Iscove's modified Dulbecco's medium. After the third generation, the BMSCs were randomly placed into the following subgroups: a control group, an osteogenic medium (OM) group, a 17β-estradiol group, an α-ZAL 10(-7) mol/L group, an α-ZAL 10(-6) mol/L group, and an α-ZAL 10(-5) mol/L group. Flow cytometry was used to identify the BMSCs collected from the bone marrow. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test was performed, and markers of the osteoblasts were measured in the different subgroups. In addition, expression of osteoprotegerin and expression of receptor activator of nuclear factor κB ligand were examined using Western blot. In contrast to the control and OM groups, BMSCs in the α-ZAL groups exhibited long fusiform shapes, and contact inhibition was observed when the cells were closely packed. After induction, the BMSCs grew well and exhibited triangular, star, polygonal, or irregular shapes. Clumps and multiple cells were evident. The trends of the proliferation and differentiation for the control, OM, 17β-estradiol, and α-ZAL groups were similar. Compared with the control and OM groups, in the α-ZAL groups the expression levels of alkaline phosphatase, procollagen type I N-terminal propeptide, bone morphogenetic protein 2, and osteocalcin were significantly increased (p < 0.05). In addition, α-ZAL inhibited osteoclastogenesis by increasing the expression of osteoprotegerin and decreasing the expression of nuclear factor κB ligand. In conclusion, α-ZAL can increase the proliferation of BMSCs and their differentiation into osteoblasts and can effectively suppress osteoclastogenesis.

The Role of miRNAs in Zearalenone-Promotion of TM3 Cell Proliferation

“…ZEA, at a low concentration, enhanced cell proliferation of a colon carcinoma cell line [12]. It has also been suggested that α-ZAL can increase the proliferation of bone marrow stromal cells and of granulosa cells [13,14].…”

Zearalenone Promotes Cell Proliferation or Causes Cell Death?

“…What’s more, several studies have indicated that the derivatives of ZEA can also stimulate cell growth. α-zearalanol (α-ZAL), one of the derivatives of ZEA, could effectively stimulate the proliferation of BMS cells, induce differentiation into osteoblasts and suppress osteoclastogenesis formation [ 12 ]. α-Zearalenol (α-ZEL), the another one derivative of ZEA, showed a strong effect of stimulating on granulosa cells, even when treated with fumonisin B1 (FB1) which could inhibit the growth of granulosa cells [ 13 ].…”

The molecular mechanism of platelet lysate promotes transformation of non-union cells into osteoblasts

“…In this study, we found that after the addition of PL, the expression levels of OPG, OCN, OPN, ALP protein were increased, but the expression level of RANKL protein did not change, the ratio of OPG/RANKL was increased. Researchers isolated various types of cells from bone delayed healing tissues, such as long fusiform or short fusiform, which were similar to nonunion cells and could differentiate to nonunion cells ( 19 ). But female nonunion cells were affected by estrogen and progesterone, influenced the reliability of experimental results.…”