The effects of β-amino-butyric acid on resistance of cucumber against root-knot nematode, Meloidogyne javanica

Abstract

In this study, the effects of b-amino-butyric acid (BABA) on root-knot nematode(Meloidogyne javanica) infection of cucumber and accumulation of total phenolic compounds, hydrogen peroxide and activity of some enzymes related to plant defense mechanisms, i.e., guaiacol peroxidase (GPOX), polyphenol oxidase (PPO), catalase (CAT) in cucumber roots infected with nematode were investigated. Results of this study show that treating the cucumber seedlings with the above elicitor significantly reduces the nematode infection level (the nematode galls, number of egg masses per plant and number of eggs per individual egg mass) compared to control. Additionally, treatment of cucumber roots by BABA and BABA ? nematode, significantly increased peroxidase, polyphenol oxidase and catalase activities in root tissues, 1 day after nematode inoculation in comparison to nematode inoculated plants as control and sterile water-treated plants. Enzyme activities reached to a maximum level at 4, 4 and 3 days after nematode inoculation, respectively. Additionally, the amount of H 2 O 2 , a product of oxidative stress, was significantly increased in the BABA and BABA ? nematode treatments in comparison to control. Such increases have occurred in two phases and maximum levels of it were observed at 5 days after inoculation. Inoculation of cucumber plants by BABA also significantly increased accumulation of total phenol in comparison to control and maximum level of it was observed at 7 days after nematode inoculation. The results suggest that the inhibitory effect of BABA on the root-knot nematode (M. javanica) may be related to its ability to enhance defense responses in the cucumber roots.

DL-β-Aminobutyric Acid-Induced Resistance in Soybean against Aphis glycines Matsumura (Hemiptera: Aphididae)

“…In this study, some defense related enzymes were activated by BABA, such as PAL, POX and POD, which were all involved in the response to SA attack. The similar phenomenon was also reported in artichoke, cucumber, apple, grapefruit [12], [16], [70], [71]. Therefore, we believe that this is a common phenomenon in BABA-treated plants.…”

“…tomato in Arabidopsis thaliana L. [15], Meloidogyne javanica in Cucumis sativus L. [16], Myzus persicae in Sinapsis alba L. [17], and Acyrthosiphon pisum in Vicia faba L. var. minor [18].…”

Induction of resistance to Meloidogyne incognita by DL-Beta amino butyric acid under salt stress condition

“…In the present study, BABA was tested against M. incognita on tomatoes. Our findings were in agreement with previous studies (Sahebani et al 2011;Oka et al 1999).…”

“…BABA has also been used as a plant inducer against root-knot nematodes in tomatoes (Oka et al 1999). Sahebani et al (2011) investigated the effects of BABA on cucumbers infected with M. javanica and the accumulation of total phenolic compounds, hydrogen peroxide and the activity of some enzymes related to plant defence mechanisms. They showed that treating the cucumber seedlings with BABA significantly reduced the nematode galls, number of egg masses per plant and number of eggs per individual egg mass compared to the control.…”

Induced systemic resistance to Meloidogyne spp by <i>β</i>,amino butyric acid in tomato

“…Induced systemic resistance to plant pathogen provides an ideal control measures against plant pathogens [3]. Chemical, physical and biological inducers to control the root knot nematodes were used [4]. In recent years, various chemical, physical and biological agents were used to induce resistance to many plant pathogens.…”