The effects of β-glucan isolated from Pleurotus ostreatus on methotrexate treatment in rats with adjuvant arthritis

Abstract

The purpose of this study was to evaluate the effect of β-(1,3/1,6)-D: -glucan isolated from Pleurotus ostreatus (β-glucan-PO) on prophylactic treatment of adjuvant arthritis (AA) with methotrexate (MTX) in rats. Groups of rats with AA were treated with methotrexate (1 mg/kg/week), β-glucan-PO (1 mg/kg every second day) or their combination for the period of 28 days from adjuvant application. Body mass, hind paw swelling, arthrogram scores and a level of serum albumin were measured as markers of inflammation and arthritis. Treatment with low dose of MTX significantly inhibited the markers of both inflammation and arthritis. MTX and its combination with β-glucan-PO significantly increased body mass of arthritic rats. β-glucan-PO administered alone significantly decreased both the hind paw swelling and arthritic score. In combination with MTX, β-glucan-PO markedly potentiated the beneficial effects of MTX, which resulted in a more significant reduction of hind paw swelling and arthritic scores. The concentration of albumin in the serum of arthritic controls was significantly lower than in healthy controls. Both MTX alone and the combination treatment with MTX + β-glucan-PO significantly inhibited the decrease in serum albumin. β-Glucan-PO increased the treatment efficacy of basal treatment of AA with MTX.

Inhibitory effect of estrogen receptor beta on P2X3 receptors during inflammation in rats

“…According to the methods reported previously [31], the paw size of rats were assessed by using Drain-age Act [32].…”

Modern Pharmacological Approaches to Therapies: Substances Tested in Animal Models of Rheumatoid Arthritis

“…® and methotrexate combination in AA The study of Rovensky et al (Rovensky et al, 2011) was focused on the effect of IMG on inflammatory and arthritic markers in rats with AA during basal treatment with MTX. The treatment was prophylactic, which means that the animals were treated immediately after administration of the adjuvant, with the same design as used in our previous experiments.…”

Effectiveness of Immunotherapies from Oyster Mushroom (Pleurotus species) in the Management of Immunocompromised Patients

“…Beta glucan from P. pulmonarius inhibited (LPS)-induced production of prostaglandin E2 (PGE2) and nitricoxide (NO) through the down-regulation of expression of COX-2 and iNOS, respectively. β-glucan also inhibited LPS dependent DNA-binding activity of AP-1 and NF-қB in RAW264.7 cells [97]. Oral administration of β-glucan markedly suppressed secretion of TNF-α and IL-6 in mice challenged with LPS in vivo.…”

“…The β-((1-3/1-6)-D-glucan isolated from P. ostreatus decreased the arthritis development in rats [96]. Jedinak et al [97] reported that β-glucan from P. pulmonarius suppressed lipopolysacharide (LPS)-induced secretion of tumor necrosis factor-α(TNF-α), interleukin-6 (IL-6), and IL-12p40 from RAW264.7 macrophages. Beta glucan from P. pulmonarius inhibited (LPS)-induced production of prostaglandin E2 (PGE2) and nitricoxide (NO) through the down-regulation of expression of COX-2 and iNOS, respectively.…”