The Effects of β-Hydroxy-β-Methylbutyrate (HMB) on Chemotaxis, Phagocytosis, and Oxidative Burst of Peripheral Blood Granulocytes and Monocytes in Goats

Abstract

The objective of this study was to determine the effect of β-hydroxy-β-methylbutyrate (HMB) on the chemotactic activity, phagocytic activity, and oxidative metabolism of peripheral blood granulocytes and monocytes in goats. Goat kids aged 30 ± 3 days were divided into two groups of 12 animals each: I—control, and II—experimental. Experimental group animals were fed a diet supplemented with HMB in the amount of 50 mg/Kg BW; whereas the diets of control goats were not supplemented. At the beginning of the experiment (day 0) and on experimental days 15, 30, and 60, blood was sampled from the jugular vein to determine and compare chemotactic activity (MIGRATEST® kit), phagocytic activity (PHAGOTEST® kit), and oxidative metabolism (BURSTTEST® kit) of peripheral blood granulocytes and monocytes by flow cytometry. The analyses of the chemotactic and phagocytic activity of granulocytes and monocytes revealed statistically higher levels of phagocytic activity in the experimental group than in the control group, as expressed by the percentage of phagocytic cells and mean fluorescence intensity. HMB also enhanced the oxidative metabolism of both granulocytes and monocytes, expressed by the rate of oxidative metabolism and mean fluorescence intensity after stimulation with Escherichia coli bacteria and PMA (4-phorbol-12-β-myristate-13-acetate).

The influence of dietary supplementation with the leucine metabolite β-hydroxy-β-methylbutyrate (HMB) on the chemotaxis, phagocytosis and respiratory burst of peripheral blood granulocytes and monocytes in calves

“…The signi cant increase in the phagocytic activity of monocytes and granulocytes in the experimental group relative to the control group was re ected by an increase in the percentage of phagocytising cells as well as an increase in the mean number of bacteria ingested per phagocyte, expressed as MFI. Similar results were noted in our previous studies of geese [32] and goats [45]. In a study by Siwicki et al [37], the potential killing activity (PKA) of granulocytes and monocytes increased by 140% in sh (rainbow trout and carp) stimulated with various doses of HMB relative to the control group.…”

The influence of dietary supplementation with the leucine metabolite β-hydroxy-β-methylbutyrate (HMB) on chemotaxis, phagocytosis and respiratory burst of peripheral blood granulocytes and monocytes in calves

“…The significant increase in the phagocytic activity of monocytes and granulocytes in the experimental group relative to the control group was reflected by an increase in the percentage of phagocytising cells as well as an increase in the mean number of bacteria ingested per phagocyte, expressed as MFI. Similar results were noted in our previous studies of geese [25] and goats [46]. In a study by Siwicki et al [38], the potential killing activity (PKA) of granulocytes and monocytes increased by 140% in fish (rainbow trout and carp) stimulated with various doses of HMB relative to the control group.…”

“…Phagocytic Activity of Blood Granulocytes and Monocytes was determined with PHAGOTEST® kit (Orpegen Pharma, Heidelberg, Germany) according to the manufacturer's specifications. The detailed method how the test was performed have been described previously [46]. Briefly, the The test allows determination of the percentage of phagocytic cells based on DHR-123 convertion to the fluorescence emitter (rhodamine 123 (R123) cation) due to the oxidation in mitochondria in the presence of H2O2 .…”

“…Briefly, the The test allows determination of the percentage of phagocytic cells based on DHR-123 convertion to the fluorescence emitter (rhodamine 123 (R123) cation) due to the oxidation in mitochondria in the presence of H2O2 . The protocol has been described in detailes previously [46].…”

The influence of dietary supplementation with the leucine metabolite β-hydroxy-β-methylbutyrate (HMB) on chemotaxis, phagocytosis and respiratory burst of peripheral blood granulocytes and monocytes in calves

“…The significant increase in the phagocytic activity of monocytes and granulocytes in the experimental group relative to the control group was reflected by an increase in the percentage of phagocytising cells as well as an increase in the mean number of bacteria ingested per phagocyte, expressed as MFI. Similar results were noted in our previous studies of geese [32] and goats [45]. In a study by Siwicki et al [37], the potential killing activity (PKA) of granulocytes and monocytes increased by 140% in fish (rainbow trout and carp) stimulated with various doses of HMB relative to the control group.…”