The effects on some non-enzymatic antioxidants and oxidative stress ofAstacus leptodactylus(Esch., 1823) of starvation periods

Abstract

This study investigated the effect of starvation (78 days) and refeeding (33 days) on the oxidative stress [malondialdehyde (MDA)] and the non‐enzymatic antioxidants [vitamin E (VE), vitamin C (VC), vitamin A (VA), beta carotene (βC) and reduced glutathione (GSH)] in the hepatopancreas, muscle and gill tissues of freshwater crayfish (Astacus leptodactylus). Crayfish were divided into three experimental groups: control (fed), starved (not fed) crayfish for 78 days and refeeding crayfish for 33 days after 78 days of starvation. The biochemical analysis of the tissues was conducted at 3, 18, 33, 48, 63 and 78 days of starvation and feeding and at 3, 18 and 33 days of refeeding. It was determined that crayfish can withstand starvation period of 78 days. In all of the periods, the MDA levels were significantly higher in the tissues of starved crayfish when compared with the control. The findings of this study demonstrate that starvation has a negative effect on the VE, VC, VA, βC and GSH levels in the crayfish. The measured parameters returned to control values after 33 days of the refeeding. Additionally, the starvation resulted in decreased levels of VE, VA and βC in the abdomen muscle of crayfish consumed by humans.

Impact of Copper Oxide Nanoparticles on freshwater crayfish, Procambarus clarkia. A combined histopathological, Biochemical and genotoxicological study

“…This decrease may be due to the intensification of turnover between reduced and oxidized glutathione under stress conditions and as a result caused an increase in the consumption of some peptides for the synthesis of heavy metalbinding proteins like metallothioneins (Radwan et al, 2010). In this regard, Barim (2018) found that GSH was significantly decreased in the hepatopancreas of freshwater crayfish (Astacus leptodactylus) upon starvation. They attributed this decrease in GSH content during exposure to starvation to an increased utilization of GSH, which can be converted into oxidized glutathione, and inefficient GSH regeneration.…”

Autophagy: a necessary defense against extreme cadmium intoxication in a multigenerational 2D experiment

“…[23][24][25] . Moreover, in 2017, from the basal strain, three additional populations were generated, which differed in terms of the Cd concentration in the food (11,22 and 44 μg of Cd per g of food (dry weight)) 29 . Prior to the present experiment, approximately 20 generations of Cd-selected insects had occurred.…”

“…Autophagy enables the maintenance of homeostasis during winter dormancy, which is associated with food and energy deficiency, because autophagy supplies cells with nutrients 7 . Starvation is considered to cause prooxidative changes 8 – 11 , generating endoplasmic reticulum (ER) stress, which is recognized as a proautophagic factor 12 , 13 . The same mechanisms explain the proautophagic action of metals such as cadmium 14 .…”

Effect of short‐term fasting and re‐feeding on growth, digestive enzyme activities and antioxidant defence in yellowfin seabream,Acanthopagrus latus(Houttuyn, 1782)

“…avoiding excessive lipid accumulation; Grigorakis & Alexis, ) for processing purposes. It has been shown that starvation can induce depletion of organ antioxidant stores and increase the generation of oxygen free radicals, particularly in liver (Barim‐Öz, ; Bayir et al, ; Furné et al, ; Morales, Pérez‐Jiménez, Carmen Hidalgo, Abellán, & Cardenete, ; Pascual, Pedrajas, Toribio, López‐Barea, & Peinado, ; Pérez‐Jiménez, Guedes, Morales, & Oliva‐Teles, ). In addition, many studies have reported that the activities of the digestive enzymes can be greatly down‐regulated in fish during fasting periods as a consequence of a reduction of mechanochemical stimulators of gastrointestinal tract (GI; e.g.…”

“…liver (Barim-Öz, 2018;Bayir et al, 2011;Furné et al, 2009;Morales, Pérez-Jiménez, Carmen Hidalgo, Abellán, & Cardenete, 2004;Pascual, Pedrajas, Toribio, López-Barea, & Peinado, 2003; Pérez-Jiménez, Guedes, Morales, & Oliva-Teles, 2007). In addition, many studies have reported that the activities of the digestive enzymes can be greatly down-regulated in fish during fasting periods as a consequence of a reduction of mechanochemical stimulators of gastrointestinal tract (GI; e.g.…”