The efficacy, biodistribution and safety of an inhibinDNAvaccine delivered by attenuatedSalmonella choleraesuis

Abstract

Summary DNA vaccines, the third‐generation vaccines, were extensively studied. The attenuated Salmonella choleraesuis (S. choleraesuis) was widely focused as a carrier to deliver DNA vaccines in the chromosome–plasmid balanced‐lethal system. The efficacy of inhibin DNA vaccine delivered by attenuated S. choleraesuis was proved in mice and cows in our previous studies. In this study, the efficacy of inhibin DNA vaccine was confirmed in rhesus monkeys. To further study the biodistribution and safety, the mice were immunized under laboratory conditions. The results of the rhesus monkeys showed the plasma IgA and IgG titres against inhibin were elevated, and the oestradiol (E2) and progesterone (P4) levels were increased with immunizing inhibin DNA vaccine. The biodistribution and safety assessment displayed the body weight, pathological change and haematology indexes where there is no significant difference between vaccinated mice and control. And the genomics analysis showed there was no integration of the inhibin gene into the mouse genome 2 months after immunization. This study indicated the inhibin DNA vaccine delivered by attenuated S. choleraesuis was safe. And this vaccine was a potential means to improve their reproductive traits in primates and other animals.

Screening and Identification of Nanobody Against Inhibin Α-Subunit from a Camelus Bactrianus Phage Display Library

“…Moreover, the idea of using inhibin immunity to increase animal fertility is emerging. Chen et al revealed that active immunization of rhesus monkeys with an inhibin DNA vaccine increases their IgA and IgG antibody titers, which is an effective method to induce ovulation in primates and promote the reproduction of endangered rhesus monkeys (Chen et al, 2018). Inhibin immunity can not only promote the development of follicles but also induce superovulation in animals.…”

DNA Vaccines—How Far From Clinical Use?

“…For oral administration of DNA, non-pathogenic ( L. lactis [ 221 ] or attenuated ( S. thyphimurium [ 222 ] and L. monocytogenes [ 223 ]) bacterial strains are often used as vectors, termed bactofection [ 224 ]. In the intestine, these bacteria may be phagocytosed directly by mucosal DC/macrophages spreading extensions into the gut lumen or after M cell-mediated transcytosis at the Peyer’s patches [ 225 ].…”