The efficacy of a sulphated polysaccharide fraction from Hypnea musciformis against diarrhea in rodents

Sousa, Nayara Alves de; Barros, Francisco Clark Nogueira; Araújo, Thiago Souza; Costa, Douglas Soares da; Souza, Luan Kelves Miranda de; Sousa, Francisca Beatriz M.; Leódido, Ana Carolina Machado; Pacífico, Dvison M.; Araújo, Simone de; Bezerra, Francisco Felipe; Freitas, Ana Lúcia Ponte; Medeiros, Jand Venes R.

doi:10.1016/j.ijbiomac.2016.02.028

Cited by 23 publications

(18 citation statements)

References 69 publications

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“…It can then be inferred that the inhibitory effects of RAG on castor oil-induced diarrhea could be due to inhibition of any of these mediators [56], which would affect the intraluminal fluid accumulation produced by castor oil. Studies by Araújo et al [32], Sousa et al [46], Leódido et al [47], and Bezerra et al [57] on the antidiarrheal actions of red algae-sulfated polysaccharides of G. cervicornis, H. musciformis, G. intermedia, and a heteropolysaccharide from cashew gum (Anacardium occidentale), respectively, showed a result similar to ours, using a similar technique. Our results showed a small difference in effect between the doses of 60 and 120 mg/kg, indicating a plateau effect towards 120 mg/kg.…”

Section: Rag Reduces Castor Oil-induced Diarrheasupporting

confidence: 86%

“…Pretreatment with naloxone did not impair the effects of RAG (60 mg/kg) treatment (30.9% inhibition), although it prevented the activity of loperamide (Figure 4a). Therefore, the antidiarrheal effect of RAG is not dependent on an opioid mechanism and a similar result has been observed with polysaccharides extracted from red algae [45][46][47]. Thus, RAG may delay gastrointestinal transit by other mechanisms that modulate gastrointestinal motility.…”

Section: Rag Effects On Gastrointestinal Motilitysupporting

confidence: 68%

“…Additionally, the determination of the solubility of RAG at different pHs was performed, and the results are provided in Table 1. RAG showed high solubility at all pH values tested, especially in acidic and basic media, corresponding with the pH found in certain regions in the gastrointestinal tract such as the stomach and the intestines [44], the main sites of action of polysaccharides tested by our research group [31,32,[45][46][47]. The structure of RAG is rich in hydroxyl (OH) groups (Figure 1a), which provides a high degree of hydration leading to greater biopolymer interaction with water and consequently greater solubility [48].…”

Section: Chemical Analysis Of Ragmentioning

confidence: 99%

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Biopolymer Extracted from Anadenanthera colubrina (Red Angico Gum) Exerts Therapeutic Potential in Mice: Antidiarrheal Activity and Safety Assessment

Araújo

Oliveira²,

Sousa

et al. 2020

Pharmaceuticals

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Anadenanthera colubrina var. cebil (Griseb.) Altschul (Fabaceae family), commonly known as the red angico tree, is a medicinal plant found throughout Brazil’s semi-arid area. In this study, a chemical analysis was performed to investigate the antidiarrheal activity and safety profile of red angico gum (RAG), a biopolymer extracted from the trunk exudate of A. colubrina. Upon FT-IR spectroscopy, RAG showed bands in the regions of 1608 cm−1, 1368 cm−1, and 1029 cm−1, which relate to the vibration of O–H water molecules, deformation vibration of C-O bands, and vibration of the polysaccharide C-O band, respectively, all of which are relevant to glycosidic bonds. The peak molar mass of RAG was 1.89 × 105 g/mol, with the zeta potential indicating electronegativity. RAG demonstrated high yield and solubility with a low degree of impurity. Pre-treatment with RAG reduced the total diarrheal stool and enteropooling. RAG also enhanced Na+/K+-ATPase activity and reduced gastrointestinal transit, and thereby inhibited intestinal smooth muscle contractions. Enzyme-Linked Immunosorbent Assay (ELISA) demonstrated that RAG can interact with GM1 receptors and can also reduce E. coli-induced diarrhea in vivo. Moreover, RAG did not induce any signs of toxicity in mice. These results suggest that RAG is a possible candidate for the treatment of diarrheal diseases.

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Section: Rag Reduces Castor Oil-induced Diarrheasupporting

confidence: 86%

Section: Rag Effects On Gastrointestinal Motilitysupporting

confidence: 68%

Section: Chemical Analysis Of Ragmentioning

confidence: 99%

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Biopolymer Extracted from Anadenanthera colubrina (Red Angico Gum) Exerts Therapeutic Potential in Mice: Antidiarrheal Activity and Safety Assessment

Araújo

Oliveira²,

Sousa

et al. 2020

Pharmaceuticals

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“…It is also supported by data that show polysaccharides from Gigartina skottsbergii, Tichocarpus crinitus, Gloiopeltis furcate, Chondrus crispus, and Halymenia durvillei, Geogiella confluens, and Cryptonemia seminervis are sulfated 15−42% (Barabanova et al 2005;Fenoradosoa et al 2009;Kolender and Matulewicz 2002;Mendes et al 2014;McCandless et al 1973;Piriz and Cerezo 1991;Yu et al 2010). In contrast, low sulfate content was detected in acidic polysaccharides from other red seaweed: 2.8% from P. decipiens (Matsuhiro and Urzua 1996), 8.4% from Grarcilaria birdiae (Souza et al 2012), 4.8% from Gracilaria cornea (Melo et al 2002), 1.2% from Gracilaria dura (Marinho-Soriano and Bourret 2005), and 5.08% from H. musciformis (Sousa et al 2016). The monosaccharide composition of SP1 and SP2 analyzed by Values were obtained by setting the sum of each mole number at 100% HPAEC-PAD revealed that galactose is the major monosaccharide (92.9% and 91.3% in mole %), while fucose (6.5% and 7.4%), glucose (1.8% and 0.6%), and xylose (0.9% and 0.7%) are present as minor components (Table 3 and Fig.…”

Section: Chemical Composition Of Spsmentioning

confidence: 99%

“…Superoxide radical scavenging activity of red algal sulfated polysaccharide has also been demonstrated (Souza et al 2007). Recently, red algal SPs showed anti-diarrheal activity (Sousa et al 2016), and also improved colitis (Brito et al 2014) in rat models. The information on biological activities of red algal polysaccharides have been reviewed and compiled (Jiao et al 2011;Usov 2011).…”

Section: Introductionmentioning

confidence: 99%

Anticancer Activity of Sulfated Polysaccharides Isolated from the Antarctic Red Seaweed Iridaea cordata

Kim¹,

Kim²,

Koo³

et al. 2016

Ocean and Polar Research

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: This study aimed to isolate and characterize sulfated polysaccharides (SPs) from Iridaea cordata and evaluate their anticancer activity. SPs of the Antarctic red seaweed were obtained by CaCl 2 (SP1) and ethanol precipitations (SP2) following diluted acid extraction at room temperature. Yields of SP1 and SP2 were approximately 14% and 23%, respectively, of the dry weight of red seaweed. The average molecular mass of the SP1 and SP2 was estimated about 1.84 × 10 3 and 1.42 × 10 3 kDa, respectively, by size-fractionation High-Performance Liquid Chromatography (HPLC). From the High-Performance AnionExchange Chromatography-Pulsed Amperometric Detection (HPAEC-PAD) analysis, the main monosaccharide was galactose with glucose and fucose as minor components. The sulfate content of SP2 (40.4%) was slightly higher than that of SP1 (33.8%). The FT-IR spectra also showed characteristic band of carrageenan-like sulfated polysaccharides. Taken together the SPs are thought to be carrageenan-like sulfated galactan. The polysaccharides (SPs) from I. cordata exhibited weak antitumor activity against PC-3 (prostate cancer), HeLa (cervical cancer), and HT-29 (human colon adenocarcinoma). To our knowledge, this is the first data on biological activity of the Antarctic red seaweed I. cordata.

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Orofacial antinociceptive effect of sulphated polysaccharide from the marine algae Hypnea pseudomusciformis in rodents

et al. 2018

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This study aimed to evaluate the antinociceptive effect of sulphated polysaccharide from the marine algae Hypnea pseudomusciformis (PLS) using rodent models of orofacial pain. Acute pain was induced by formalin, capsaicin, cinnamaldehyde, acidified saline or glutamate (cutaneous modes) and hypertonic saline (corneal model). In one experiment, animals were pretreated with ruthenium red, glibenclamide, naloxone, L-NAME, methylene blue or ketamine to investigate the mechanism of antinociception. In another experiment, animals pretreated with PLS or saline were submitted to the temporomandibular joint formalin test. In yet another, animals were submitted to craniofacial pain induced by mustard oil. Motor activity was evaluated with the open-field test. Cytotoxicity and antioxidant activities were also assessed. Pre-treatment with PLS significantly reduced nociceptive behavior associated with acute pain. Antinociception was effectively reduced, but not inhibited, by ruthenium red and ketamine. L-NAME and glibenclamide enhanced the PLS effect. PLS antinociception was resistant to methylene blue, naloxone and heating. PLS presented no cytotoxicity or antioxidant properties. Our results confirm the potential pharmacological relevance of PLS as an inhibitor of orofacial nociception in acute pain probably mediated by glutamatergic, nitrergic, TRPs and K + ATP pathways.

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The efficacy of a sulphated polysaccharide fraction from Hypnea musciformis against diarrhea in rodents

Cited by 23 publications

References 69 publications

Biopolymer Extracted from Anadenanthera colubrina (Red Angico Gum) Exerts Therapeutic Potential in Mice: Antidiarrheal Activity and Safety Assessment

Biopolymer Extracted from Anadenanthera colubrina (Red Angico Gum) Exerts Therapeutic Potential in Mice: Antidiarrheal Activity and Safety Assessment

Anticancer Activity of Sulfated Polysaccharides Isolated from the Antarctic Red Seaweed Iridaea cordata

Orofacial antinociceptive effect of sulphated polysaccharide from the marine algae Hypnea pseudomusciformis in rodents

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