Different methods to biosynthesis of Gold nanoparticles was done with using cyanobacterium Lyngbya sp. , and salt gold (HAuCL4) and evaluate their antibacterial activity .Gold nanoparticles were prepared in different ways ,dry and fresh weight of this cyanobacterium to obtain aqueous extract. The synthesized nanoparticles were characterized using analytical techniques such as UV-Visible spectroscopy, SEM (Scanning Electron Microscopy), FTIR (Fourier Transform Infrared) and XRD (X-Ray Diffraction). UV-visible spectroscopy show a Surface Plasmon Resonance (SPR) peak at (532- 552) nm; confirmed by (SEM) image revealed the spherical shape and the size range from (39.1-61.9) and (59.4-84.0) nm for dry, wet aqueous extract respectively. FTIR result showed containing of ( phenol ,protein and carbonyl) as afunctional group. The crystalline nature of the nanoparticles was evident from Peaks in the (XRD) pattern. biosynthesis