The efficacy of curcumin in managing acute inflammation pain on the post‐surgical removal of impacted third molars patients: A randomised controlled trial

Zhou

Cell Biology International

et al. 2021

Articular cartilage damage and chondrocyte apoptosis are common features of rheumatoid arthritis and osteoarthritis. Recently, curcumin has been reported to exhibit protective effects on degeneration in articular cartilage diseases. However, the effects and mechanisms of curcumin on articular chondrocyte injury remain to be elucidated. The aim of the present study is to investigate the chondroprotective mechanisms of curcumin on interleukin‐1β (IL‐1β)‐induced chondrocyte apoptosis in vitro. The results revealed that IL‐1β decreased cell viability and induced apoptosis in primary articular chondrocytes. Curcumin pretreatment reduced IL‐1β‐induced articular chondrocyte apoptosis. In addition, treatment with curcumin increased autophagy in articular chondrocytes and protected against IL‐1β‐induced apoptosis. The curcumin‐mediated protection against IL‐1β induced apoptosis was abolished when cells were treated with the autophagy inhibitor 3‐methyladenine or transfected with Beclin‐1 small interfering RNA. Furthermore, IL‐1β stimulation significantly increased the phosphorylation levels of nuclear factor (NF)‐κB p65 and glycogen synthase kinase‐3β, and decreased the phosphorylation levels of β‐catenin in articular chondrocytes, and these alterations to the phosphorylation levels were partly reversed by treatment with curcumin. Dual‐luciferase and electrophoretic mobility shift assays demonstrated that IL‐1β increased NF‐κB p65 promoter activity in chondrocytes, and this was also reversed by curcumin. Pretreatment with the NF‐κB inhibitor pyrrolidine dithiocarbamate enhanced the protective effects of curcumin on chondrocyte apoptosis, but Wnt/β‐catenin inhibitor, XAV‐939, did not exhibit this effect. Molecular docking and dynamic simulation studies results showed that curcumin could bound to RelA (p65) protein. These results indicate that curcumin may suppress IL‐1β‐induced chondrocyte apoptosis through activating autophagy and restraining NF‐κB signaling pathway.

Section: Discussionmentioning

confidence: 99%

Curcumin ameliorates IL‐1β‐induced apoptosis by activating autophagy and inhibiting the NF‐κB signaling pathway in rat primary articular chondrocytes

Zhou

Cell Biology International

et al. 2021

“…Other investigations, demonstrated that the use of non-steroidal anti-in ammatory drugs can prevent the anesthesia and surgery-associated memory de cits probably via their pain-alleviating effects (Shen et al 2013;Kawano et al 2014). In the same perspective, curcumin, an active ingredient of plant Curcuma longa, is apparently effective in treating POP related to GA and surgery in human (Agarwal et al 2011;Maulina et al 2018), and in mice (Sahbaie et al 2014). As well as suppressing of neuro-in ammatory activation associated with neurodegenerative diseases (Sundaram et al 2017), and preventing anesthetics exposure-induced POCD in mice (Ji et al 2015;Wu et al 2017).…”

Section: Discussionmentioning

confidence: 98%

Postoperative cognitive recovery in children after both general and locoregional anesthesia and surgery

Hammoumi

Dahchour

Chhiti

et al. 2020

Preprint

Background and objectivesRecent researches have demonstrated that there is a progressive impairment in neurocognitive function following general anesthesia and surgery, and particularly, have evidenced that anesthetics impaired mechanisms of learning and memory, for days to months, in both adults and children.This study aimed to evaluate the influence of different types of anesthesia (General or Locoregional) on cognitive recovery by trial and error method, and also take into consideration other factors that would have an impact on the cognitive performance after anesthesia and surgery in children.MethodsIn the present cross-sectional study, 64 young children, aged between 10 and 15 years, have passed the CALM test (software based on trial and error method) 24h after their surgery, and the operating file filled by the anesthetist. The statistical analysis includes a descriptive part of our sample, a univariate, and multivariate analysis of the results of the CALM test and the various medical factors and lifestyles.Results and conclusionsThe cognitive performance of patients exposed to locoregional anesthesia (81.3%) was higher (p=0.01) when compared to patients under general anesthesia (34.4%). Both agitation (Neurological state) and postoperative pain significantly (p=0.02) altered the cognitive performance of patients. However, none of the other factors assessed such as gender, environmental living, schooling, pre-anesthetic consultation, and the type of intervention affects the postoperative cognitive performance of patients.Locoregional anesthesia has less effect on cognitive recovery when compared to general anesthesia. Both the postoperative pain and agitation alter cognitive performances of operated children.

“…In the present study, the Curcumin also presented a favorable tissue response at the final period of 63 days. This can be attributed to the fact that, as described above, the Curcumin is able to regulate an amount of transcription factors, cytokines, kinases, adhesion molecules, and enzymes related to inflammation process [29].…”

Section: Discussionmentioning

confidence: 98%

Photosensitizers and Exposure Times To Light Showed Tissue Compatibility In Isogenic Mice

Oliveira

Pucinelli

et al. 2021

BDS

Objective: The aim of this study was to evaluate the subcutaneous tissue response after different protocols to photodynamic therapy (PDT). In Phase 1, were tested the diode laser (used for 1min) associated to the photosensitizer phenothiazine chloride solution (PCS) in different concentrations. In Phase 2 – the diode laser and LED were tested associated to two different photosensitizers, PCS and Curcumin, in different exposure times of light application. Material and Methods: After 7, 21 and 63-days the animals were euthanized and the subcutaneous tissue processed to histological analysis. Qualitative and semi-quantitative descriptions of the inflammatory process and immunohistochemical technique were performed. The obtained data were analyzed by Kruskal-Wallis and Dunn’s post-test (alpha = 0.5). Results: On Phase 1, the tissue response was very similar among the groups. For the inflammatory infiltrate, PCS with concentration of 10mg/mL exhibited the most intense reaction (p > 0.05). On Phase 2, at 7-days period, the analyzed parameters presented small magnitude and after 21 and 63-days, all the parameters demonstrated tissue compatibility. Conclusion: Both photosensitizers presented proper tissue compatibility regardless the different concentrations used on Phase 1 and different durations of light exposure on Phase 2. Keywords Photodynamic therapy; Phenothiazine chloride solution; Curcumin; Isogenic mice; Subcutaneous tissue.