The efficacy of eugenol and tricaine methanesulphonate as anaesthetics for juvenile Chinese sea bass (Lateolabrax maculatus) during simulated transport

Abstract

Summary The purpose of this study was to evaluate the anaesthesia effects of eugenol and MS‐222 sedatives applied on juvenile Lateolabrax maculatus during simulated transport. In experiment 1, the juveniles were divided into two groups, with seven concentrations tested on each group (eugenol [4, 6, 8, 10, 12, 14 and 16 mg/L] and MS‐222 [20, 30, 40, 50, 60, 70 and 80 mg/L]). Induction and recovery times were recorded. The time for anaesthesia was shortened, and the time for complete recovery was prolonged as the anaesthetic concentration increased. The optimal transport concentration for each anaesthetic tested was 6 mg/L of eugenol and 30 mg/L for MS‐222. In experiment 2, the 5‐hr simulated transport test showed that the survival rate of L. maculatus juveniles with anaesthesia was 100%, and without anaesthesia, survival was 60%. After 24 hr of recovery following transport, the fish showed 100% survival for the group with added anaesthetic and 40% for the group without added anaesthetic. Compared to the non‐anaesthetized groups, the anaesthetized transport groups showed significant increases in the levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) (p < 0.05). The levels of AST, ALT and alkaline phosphatase (AKP) were significantly higher in the MS‐222 transport group than in the eugenol transport group (p < 0.05). The levels of AKP were significantly higher in the non‐anaesthetized transport group than in the anaesthetized group (p < 0.05). According to the present experiment results, eugenol was an efficient anaesthetic in L. maculatus, and we recommend eugenol instead of MS‐222 as an anaesthetic for the short‐time transport of L. maculatus.

Anaesthetic effects of eugenol on preservation and transportation of yellow catfish ( Pelteobagrus fulvidraco )

“…In addition, the increased dosage of eugenol resulted in a quicker response to induction and slower recovery in yellow catfish. This might be that the sharp diffusion gradient between the water and fish was created by the increasing dose of anaesthetic (Hasimuna et al, 2020), and similar findings about eugenol have been well documented in Chinese sea bass (L. maculatus) (Wang et al, 2018), crucian carp (Carassius auratus) (Le et al, 2018) and common carp (Cyprinus carpio) (Yousefi et al, 2018). These results may suggest that the exposure of eugenol can induce fish anaesthesia in a dose-dependent manner.…”

Evaluation of eugenol as a sedative for the transportation of common snook Centropomus undecimalis (Bloch, 1792)

“…Full induction (motion and ventilation reduction and reception only to gross stimulation) was reached at 118.62 ± 8.23 s, which is within the optimal induction time reported for fish (less than 3 min) (Gilderhus & Marking, 1987). In general, studies register higher eugenol concentrations for the sedation of fish (Bolasina et al, 2017;Wenhao et al, 2019), which suggests the specificity of the response of fish to this chemical and the effectiveness of eugenol on common snook juveniles. Once in the recovery tank, all fish regained normal behaviour in 189.32 ± 26.67 s, with no significant differences registered among experimental units (p = 0.85).…”

Preservative Effects of Gelatin Active Coating Containing Eugenol and Higher CO2 Concentration Modified Atmosphere Packaging on Chinese Sea bass (Lateolabrax maculatus) during Superchilling (−0.9 °C) Storage

“…In the last several years, the annual output of sea bass culture has reached more than 100,000 tons [1]. The consumption of sea bass has also increased due to its nutritional characteristics as well as its benefits to consumer health [2]. However, fresh sea bass is high in protein and water and is prone to corruption and deterioration in the process of transportation, storage, processing, and marketing [3,4].…”