The efficacy of<i>Mycoplasma gallisepticum</i>K-strain live vaccine in broiler and layer chickens

Ferguson‐Noel, Naola; Williams, Susan M.

doi:10.1080/03079457.2015.1005054

Cited by 23 publications

(11 citation statements)

References 41 publications

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“…gallisepticum vaccination are bacterins, live vaccines, and an M. gallisepticum antigen expressing recombinant fowl pox vaccine (4). Currently, the worldwide used live M. gallisepticum vaccine strains are the 6/85 (Nobilis MG6/85; MSD Animal Health), ts-11 (VaxSafe MG; Bioproperties Pty Ltd.), and the F strain (Cevac MG-F; Ceva, Inc.).…”

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Development of Molecular Methods for Rapid Differentiation of Mycoplasma gallisepticum Vaccine Strains from Field Isolates

et al. 2019

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Mycoplasma gallisepticum is among the most economically significant mycoplasmas causing production losses in poultry. Seven melt-curve and agarose gel-based mismatch amplification mutation assays (MAMAs) and one PCR are provided in the present study to distinguish the M. gallisepticum vaccine strains and field isolates based on mutations in the crmA, gapA, lpd, plpA, potC, glpK, and hlp2 genes. A total of 239 samples (M. gallisepticum vaccine and type strains, pure cultures, and clinical samples) originating from 16 countries and from at least eight avian species were submitted to the presented assays for validation or in blind tests. A comparison of the data from 126 samples (including sequences available at GenBank) examined by the developed assays and a recently developed multilocus sequence typing assay showed congruent typing results. The sensitivity of the melt-MAMA assays varied between 10 1 and 10 4 M. gallisepticum template copies/reaction, while that of the agarose-MAMAs ranged from 10 3 to 10 5 template copies/reaction, and no cross-reactions occurred with other Mycoplasma species colonizing birds. The presented assays are also suitable for discriminating multiple strains in a single sample. The developed assays enable the differentiation of live vaccine strains by targeting two or three markers/vaccine strain; however, considering the high variability of the species, the combined use of all assays is recommended. The suggested combination provides a reliable tool for routine diagnostics due to the sensitivity and specificity of the assays, and they can be performed directly on clinical samples and in laboratories with basic PCR equipment.

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confidence: 99%

Development of Molecular Methods for Rapid Differentiation of Mycoplasma gallisepticum Vaccine Strains from Field Isolates

et al. 2019

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“…These results confirm, as reported for the ts-11 vaccine [ 27 ], that the MS-H seemed incapable of spreading and colonizing the oviduct, which was also shown by the negative results of the oviducts and, therefore, of the embryonated eggs. The laboratory findings of the present study suggest that there was no vertical spread of the MS vaccine, but further large scale studies should be carried out in view of the recent reports of MG vaccine transmission in the USA [ 28 ].…”

Section: Discussionmentioning

confidence: 87%

Application of different laboratory techniques to monitor the behaviour of a Mycoplasma synoviae vaccine (MS-H) in broiler breeders

Moronato

Cecchinato

Facchetti³

et al. 2018

BMC Vet Res

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BackgroundMycoplasma synoviae (MS) is a major poultry pathogen which causes severe economic losses in all the productive sectors. The prevalence of MS in European countries has increased in the last few years, leading to greater attention to the available methods to prevent its spread. The main strategy currently applied for its containment is the development and maintenance of MS-free breeder flocks. A live MS vaccine (MS-H) obtained by mutagenizing an Australian field strain has recently been introduced in Italy. The aim of the present study was to evaluate the vaccine behaviour in broiler breeder groups at different production stages and the effectiveness of the available laboratory tests in discriminating the MS-H from a field strain.ResultsThe vaccine diffused extensively through the population, shown by the wide serological response (over 80% of positive samples in RSA and 85% in ELISA), the high serological titres, the positivity of all the tracheal samples collected during the production phase by MS PCR and the positivity by cultivation from tracheal swabs at the end-point (55 weeks after vaccination). In contrast, only one swab from a sternal bursa was positive in MS PCR, while all the joint and oviduct samples were negative. There was no evidence of vertical transmission. Different genotyping techniques were used to achieve a clear classification of the MS positive samples. The vlhA and the obg gene analysis showed that most of the strains were homologous with the vaccine, but some ambiguous samples were further investigated with the multi locus sequence typing (MLST) scheme which confirmed the homology.ConclusionsThe development of a multi-technique approach to monitor vaccinated avian flocks, based both on serological and biomolecular methods, is advised as well as the use of effective genotyping techniques to analyse the MS strains circulating in high densely populated poultry areas.Electronic supplementary materialThe online version of this article (10.1186/s12917-018-1669-8) contains supplementary material, which is available to authorized users.

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“…For example, MG was considered as nonpathogenic, isolated from the respiratory and reproductive tract of chickens ( Wang et al., 1990 ), and evaluated as a possible vector for MG antigen expression ( Evan et al., 2005 ). Recently, the efficacy of K-strain as a live-attenuated vaccine was reported to be safe in chickens ( Ferguson-Noel and Williams, 2015 ). Moreover, another study compared the protection elicited by K-strain and ts-11 in layers and efficacy of K-strain and F-strain in broilers.…”

Section: Mg Vaccine Candidatesmentioning

confidence: 99%

“…Moreover, another study compared the protection elicited by K-strain and ts-11 in layers and efficacy of K-strain and F-strain in broilers. Their results showed that K-strain vaccine has equivalent efficacy and potential to protect vaccinated birds from field challenge ( Ferguson-Noel and Williams, 2015 ). F strain vaccine was administered in ovo to layers to reduce cost of labor and evaluate it as an effective vaccination to control MG infection in commercial layer flocks.…”

Section: Mg Vaccine Candidatesmentioning

confidence: 99%

Current status of vaccine research, development, and challenges of vaccines for Mycoplasma gallisepticum

Ishfaq

Khan

et al. 2020

Poultry Science

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Mycoplasma gallisepticum ( MG ) is an important avian pathogen that causes significant economic losses in the poultry industry. Surprisingly, the limited protection and adverse reactions caused by the vaccines, including live vaccines, bacterin-based (killed) vaccines, and recombinant viral vaccines is still a major concern. Mycoplasma gallisepticum strains vary in infectivity and virulence and infection may sometimes unapparent and goes undetected. Although extensive research has been carried out on the biology of this pathogen, information is lacking about the type of immune response that confers protection and selection of appropriate protective antigens and adjuvants. Regardless of numerous efforts focused on the development of safe and effective vaccine for the control of MG, the use of modern DNA vaccine technology selected in silico approaches for the use of conserved recombinant proteins may be a better choice for the preparation of novel effective vaccines. More research is needed to characterize and elucidate MG products modulating MG-host interactions. These products could be used as a reference for the preparation and development of vaccines to control MG infections in poultry flocks.

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The efficacy ofMycoplasma gallisepticumK-strain live vaccine in broiler and layer chickens

Cited by 23 publications

References 41 publications

Development of Molecular Methods for Rapid Differentiation of Mycoplasma gallisepticum Vaccine Strains from Field Isolates

Development of Molecular Methods for Rapid Differentiation of Mycoplasma gallisepticum Vaccine Strains from Field Isolates

Application of different laboratory techniques to monitor the behaviour of a Mycoplasma synoviae vaccine (MS-H) in broiler breeders

Current status of vaccine research, development, and challenges of vaccines for Mycoplasma gallisepticum

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