The efficacy of soft contact lens disinfection solutions against Serratia marcescens and Pseudomonas aeruginosa

Parment, Per Arne; Colucci, Betina; Nyström, Bertil

doi:10.1111/j.1600-0420.1996.tb00083.x

Cited by 14 publications

(12 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…OptiFree® Express®, containing Polyquad® and Aldox® as antimicrobials, was more effective against both bacterial strains than ReNu® MultiPlus®, containing Dymed® and Poloxamine® or SoloCare Aqua™ containing polyhexanide. Several studies have compared the antimicrobial efficacies of these solutions, with remarkably contrasting results 19, 21, 30–33. Dannelly and Waworuntu19 found a lower log reduction of planktonic bacteria suspended in ReNu® MultiPlus®, if it was first preincubated with a contact lens for 7 days, that is, much longer than overnight exposure as used here.…”

Section: Discussionmentioning

confidence: 85%

Bacterial transmission from lens storage cases to contact lenses—Effects of lens care solutions and silver impregnation of cases

et al. 2008

View full text Add to dashboard Cite

The killing efficacies of multipurpose lens care solutions on planktonic and biofilm bacteria grown in polypropylene contact lens storage cases with and without silver impregnation and effects on bacterial transmission from storage cases to silicone hydrogel contact lenses were investigated. For transmission studies, biofilms of Staphylococcus aureus 835 or Pseudomonas aeruginosa no. 3 were grown on lens storage cases and incubated with a contact lens in different multipurpose lens care solutions (Opti-Free(R)Express(R), ReNu(R) MultiPlus(R), and SoloCare Aquatrade mark) or 0.9% NaCl. In addition, planktonic bacteria were directly suspended in multipurpose solutions and their killing efficacies were determined. The numbers of transmitted live and dead bacteria on the lenses were measured using a combination of plate counting and fluorescence microscopy. The highest killing efficacies were shown by Opti-Free(R) Express(R) for planktonic as well as for biofilm bacteria. Silver impregnation of lens cases in combination with the prescribed solution increased the killing efficacy for P. aeruginosa in biofilms, whereas effects for S. aureus were minor. Lowest numbers of live and dead bacteria were transmitted to a lens in Opti-Free(R) Express(R) multipurpose solution, with no significant differences between lens types and no effects of silver impregnation. (c) 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 2008.

show abstract

Section: Discussionmentioning

confidence: 85%

Bacterial transmission from lens storage cases to contact lenses—Effects of lens care solutions and silver impregnation of cases

et al. 2008

View full text Add to dashboard Cite

show abstract

“…The isolation rate of S. marcescens from contact lens-coupled corneal infections ranges from 5 to 28%; almost comparable to the isolation rate of P. aeruginosa in contact lens-related keratitis [69,167]. S. marcescens is a motile gram negative rod abundantly dispersed in nature and contributes to contact lens-related keratitis [141,142].…”

Section: Bacterial Keratitismentioning

confidence: 87%

Pathogenesis of microbial keratitis

Lakhundi

Siddiqui

Khan

2017

Microbial Pathogenesis

181

121

View full text Add to dashboard Cite

Microbial keratitis is a sight-threatening ocular infection caused by bacteria, fungi, and protist pathogens. Epithelial defects and injuries are key predisposing factors making the eye susceptible to corneal pathogens. Among bacterial pathogens, the most common agents responsible for keratitis include Staphylococcus aureus, Pseudomonas aeruginosa, Streptococcus pneumonia and Serratia species. Fungal agents of corneal infections include both filamentous as well as yeast, including Fusarium, Aspergillus, Phaeohyphomycetes, Curvularia, Paecilomyces, Scedosporium and Candida species, while in protists, Acanthamoeba spp. are responsible for causing ocular disease. Clinical features include redness, pain, tearing, blur vision and inflammation but symptoms vary depending on the causative agent. The underlying molecular mechanisms associated with microbial pathogenesis include virulence factors as well as the host factors that aid in the progression of keratitis, resulting in damage to the ocular tissue. The treatment therefore should focus not only on the elimination of the culprit but also on the neutralization of virulence factors to minimize the damage, in addition to repairing the damaged tissue. A complete understanding of the pathogenesis of microbial keratitis will lead to the rational development of therapeutic interventions. This is a timely review of our current understanding of the advances made in this field in a comprehensible manner. Coupled with the recently available genome sequence information and high throughput genomics technology, and the availability of innovative approaches, this will stimulate interest in this field.

show abstract

“…Serratia marcescens has previously been found to be resistant to contact lens disinfecting solutions containing polyquaternium‐1 (Parment et al. 1996; Lever & Miller 1999; Hume et al.…”

Section: Discussionmentioning

confidence: 99%

“…2007). Several studies have shown that polyquaternium solutions may have delayed inactivation kinetics against S. marcescens (Parment et al. 1996; Parment 1997; Lever & Miller 1999; Santodomingo‐Rubido et al.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Detection of virulence factors in Serratia strains isolated from contact lens‐associated corneal ulcers

Pinna

Usai

Sechi

et al. 2011

Acta Ophthalmologica

View full text Add to dashboard Cite

. Purpose: This paper reports on the microbiological findings pertaining to three Serratia isolates from soft contact lens‐related corneal ulcers, which represent a complication of contact lens wear reported with increasing frequency. Methods: Bacterial identification and antibiotic susceptibility testing were performed using the Vitek system. Serratia’s ability to form biofilm, produce gelatinase, elastase and alkaline protease, and invade human corneal epithelial (HCE) cells was investigated. The isolates’ susceptibility to the following disinfectants was tested: (a) ReNu MultiPlus®, containing polyaminopropyl biguanide 0.0001%; (b) Opti‐Free Express®, containing polyquaternium‐1 0.001% and myristamidopropyldimethylamine 0.0005%; (c) Opti‐Free Replenish®, containing polyquaternium‐1 0.001% and myristamidopropyldimethylamine 0.0005%, and (d) Oxysept Comfort®, a one‐step 3% hydrogen peroxide‐catalase system. Results: Two Serratia marcescens and one Serratia liquefaciens were identified. All the strains were susceptible to aminoglycosides and fluoroquinolones. No isolate formed biofilm or significantly invaded HCE cells; all produced alkaline protease and gelatinase, but not elastase. Opti‐Free Express® and Opti‐Free Replenish® were active against S. liquefaciens, but failed to kill the S. marcescens isolates within the minimum recommended time (6 hours). ReNu MultiPlus® needed 6 hours to kill one strain of S. marcescens, which is 2 hours more than recommended. Conversely, Oxysept Comfort® was always effective within the minimum recommended time (6 hours). Conclusions: The ability to produce alkaline protease and gelatinase may play a major role in the pathogenesis of contact lens‐related Serratia keratitis. Several types of contact lens solutions may be ineffective in eradicating Serratia from contaminated contact lens cases within the minimum recommended time. Only exposure to Oxysept Comfort® was always effective against Serratia in this study.

show abstract

The efficacy of soft contact lens disinfection solutions against Serratia marcescens and Pseudomonas aeruginosa

Cited by 14 publications

References 16 publications

Bacterial transmission from lens storage cases to contact lenses—Effects of lens care solutions and silver impregnation of cases

Bacterial transmission from lens storage cases to contact lenses—Effects of lens care solutions and silver impregnation of cases

Pathogenesis of microbial keratitis

Detection of virulence factors in Serratia strains isolated from contact lens‐associated corneal ulcers

Contact Info

Product

Resources

About