The efficacy of the use of IR laser phototherapy associated to biphasic ceramic graft and guided bone regeneration on surgical fractures treated with miniplates: a histological and histomorphometric study on rabbits

Abstract

The aim of the present study was to assess, by light microscopy and histomorphometry, the repair of surgical fractures fixed with internal rigid fixation (IRF) treated or not with IR laser (λ780 nm, 50 mW, 4 × 4 J/cm(2) = 16 J/cm(2), ϕ = 0.5 cm(2), CW) associated or not to the use of hydroxyapatite and guided bone regeneration. Surgical tibial fractures were created under general anesthesia on 15 rabbits that were divided into 5 groups, maintained on individual cages, at day/night cycle, fed with solid laboratory pelted diet, and had water ad libidum. The fractures in groups II, III, IV, and V were fixed with miniplates. Animals in groups III and V were grafted with hydroxyapatite and GBR technique used. Animals in groups IV and V were irradiated at every other day during two weeks (4 × 4 J/cm(2), 16 J/cm(2) = 112 J/cm(2)). Observation time was that of 30 days. After animal death, specimens were taken, routinely processed to wax, cut and stained with HA and Sirius red, and used for histological assessment. The results of both analyses showed a better bone repair on all irradiated subjects especially when the biomaterial and GBR were used. In conclusion, the results of the present investigation are important clinically as they are suggestive that the association of hydroxyapatite, and laser light resulted in a positive and significant repair of complete tibial fractures treated with miniplates.

Effects of photobiomodulation on bone defects grafted with bone substitutes: A systematic review of in vivo animal studies

“…One study was excluded because there was no bone defect [20] while the other study excluded as no bone substitute utilized for grafting procedures [26]. Furthermore, three studies were excluded due to the following reasons: the recipient site was an extraction socket or bone fracture site [32–34], PBMT was not utilized [35], in vitro study analysis [25]. Eight studies were excluded as they were based on utilization of autogenous bone grafts for bone repair and regeneration [24, 27, 36–41] (inter‐reviewer agreement, κ = 0.94).…”

“…One study was excluded because there was no bone defect [20] while the other study excluded as no bone substitute utilized for grafting procedures [26]. Furthermore, three studies were excluded due to the following reasons: the recipient site was an extraction socket or bone fracture site [32][33][34], PBMT was not utilized [35], in…”

Validation of the laser‐induced fluorescence effect of aromatic amino acids, protoporphyrin IX, and tetracycline hydrochloride on hydroxyapatite

“…However, intrinsic enamel stains and “irregular” enamel structures without dental caries also produce very high readings by the DD device. [ 3,16–18 ] Furthermore, previous experiment had shown that aromatic amino acids (including tryptophan, phenylalanine, and tyrosine) are responsible for the fluorescence and colour properties of synthesised hydroxyapatite as the simplified model of teeth but non‐aromatic amino acids. [ 11 ] Therefore, we hypothesised that aromatic amino acids may also be the cause of DD fluorescence and the increased readings.…”

“…During energy exchange, different molecules acquire energy and produce different optical frequencies, which is known as the Raman shift, and from these data, we can obtain information on the molecular vibration and rotation. [ 16–18 ] Our research group found that Raman spectroscopy can detect changes in the content of organic matter and inorganic matter in teeth before and after bleaching and that the Raman fluorescence intensity (FI) correlates with tooth colour. [ 14,19–21 ]…”

Cuttlebone used as a bone xenograft in bone healing

“…A number of research studies have focused on the healing of bone defects with remodelling (Bruder et al 1998;Khan 2000;Pinheiro et al 2014;Thormann et al 2014). In recent years, many commercial bone graft materials have been introduced.…”