The efficiency of photothermal action of gold shell-isolated nanoparticles against tumor cells depends on membrane interactions

Camacho, Sabrina A.; Kobal, Mirella B.; Moreira, Lucas G.; Bistaffa, Maria J.; Roque, Thamires C.; Pazin, Wallance Moreira; Toledo, Karina Alves; Oliveira, Osvaldo N.; Aoki, Pedro H.B.

doi:10.1016/j.colsurfb.2021.112301

Cited by 8 publications

(5 citation statements)

References 111 publications

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“…Such area increase, while not highly significant, is likely attributed to the inherent complexity of the cell extract. Similar challenges have been documented in recent studies involving tumor lipid extract monolayers, where obtaining more pronounced effects proved intricate. , An illustrative model of the binding mechanism and impact of the photoreaction is presented in Figure b.…”

Section: Resultsmentioning

confidence: 98%

“…Similar challenges have been documented in recent studies involving tumor lipid extract monolayers, where obtaining more pronounced effects proved intricate. 71, 72 An illustrative model of the binding mechanism and impact of the photoreaction is presented in Figure 4b. Irradiation of Caco-2 lipid extract monolayers containing AuSHINRs@TBO (1:1 v/v) causes significant changes in the PM-IRRAS, especially in the head groups, as shown in Figure 3.…”

Section: T H I S C O N T E N T Imentioning

confidence: 99%

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Enhancing Phototoxicity in Human Colorectal Tumor Cells Through Nanoarchitectonics for Synergistic Photothermal and Photodynamic Therapies

Mendes de Almeida Junior,

Ferreira,

Camacho

et al. 2024

ACS Appl. Mater. Interfaces

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Phototherapies are promising for noninvasive treatment of aggressive tumors, especially when combining heat induction and oxidative processes. Herein, we show enhanced phototoxicity of gold shell-isolated nanorods conjugated with toluidine blue-O (AuSHINRs@TBO) against human colorectal tumor cells (Caco-2) with synergic effects of photothermal (PTT) and photodynamic therapies (PDT). Mitochondrial metabolic activity tests (MTT) performed on Caco-2 cell cultures indicated a photothermal effect from AuSHINRs owing to enhanced light absorption from the localized surface plasmon resonance (LSPR). The phototoxicity against Caco-2 cells was further increased with AuSHINRs@TBO where oxidative processes, such as hydroperoxidation, were also present, leading to a cell viability reduction from 85.5 to 39.0%. The molecular-level mechanisms responsible for these effects were investigated on bioinspired tumor membranes using Langmuir monolayers of Caco-2 lipid extract. Polarization-modulation infrared reflection−absorption spectroscopy (PM-IRRAS) revealed that the AuSHINRs@TBO incorporation is due to attractive electrostatic interactions with negatively charged groups of the Caco-2 lipid extract, resulting in the expansion of surface pressure isotherms. Upon irradiation, Caco-2 lipid extract monolayers containing AuSHINRs@TBO (1:1 v/v) exhibited ca. 1.0% increase in surface area. This is attributed to the generation of reactive oxygen species (ROS) and their interaction with Caco-2 lipid extract monolayers, leading to hydroperoxide formation. The oxidative effects are facilitated by AuSHINRs@TBO penetration into the polar groups of the extract, allowing oxidative reactions with carbon chain unsaturations. These mechanisms are consistent with findings from confocal fluorescence microscopy, where the Caco-2 plasma membrane was the primary site of the cell death induction process.

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Section: Resultsmentioning

confidence: 98%

Section: T H I S C O N T E N T Imentioning

confidence: 99%

Enhancing Phototoxicity in Human Colorectal Tumor Cells Through Nanoarchitectonics for Synergistic Photothermal and Photodynamic Therapies

Mendes de Almeida Junior,

Ferreira,

Camacho

et al. 2024

ACS Appl. Mater. Interfaces

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“…The zeta potential was also affected, especially from the AuNPs (−32.4 ± 0.6 mV) to the SERRS nanoprobes (−18 ± 2 mV), whose increase is related to the reduction of negative charges around the nanoparticles. The AuNPs synthesized via citrate are negatively charged [ 42 ] and the cationic MB [ 54 ] and inert silica shell [ 55 , 56 ] decrease the surface charge. After functionalization with the antibodies a slight decrease is noted in zeta potential (−22 ± 1 mV).…”

Section: Resultsmentioning

confidence: 99%

Immunoassay platform with surface-enhanced resonance Raman scattering for detecting trace levels of SARS-CoV-2 spike protein

Bistaffa

Camacho

Pazin

et al. 2022

Talanta

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“…Amongst the metallic nanoparticles, gold nanoparticles (AuNPs) stand out as the most investigated in the last decades. AuNPs have been pointed out as potential agents for imaging, sensoring, photothermia, and antimicrobial applications providing suitable therapeutic efficiency and low toxicity [ 10 , 11 , 12 , 13 ]. The AuNPs have also been reported as useful antitumoral therapeutics, for example, against breast cancer cells.…”

Section: Introductionmentioning

confidence: 99%

Co-Functionalization of Gold Nanoparticles with C7H2 and HuAL1 Peptides: Enhanced Antimicrobial and Antitumoral Activities

et al. 2022

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The functionalization of nanoparticles with therapeutic peptides has been pointed out as a promising strategy to improve the applications of these molecules in the field of health sciences. Peptides are highly bioactive but face several limitations such as low bioavailability due to the difficulty of overcoming the physiological barriers in the body and their degradation by enzymes. In this work, gold nanoparticles (AuNPs) were co-functionalized with two therapeutic peptides simultaneously. The peptides from the complementary determining region of monoclonal antibodies, composed of the amino acid sequences YISCYNGATSYNQKFK (C7H2) and RASQSVSSYLA (HuAL1) were chosen for having exhibited antitumor and antimicrobial activity before. The peptides-conjugated AuNPs were characterized regarding size, morphology, and metal concentration by using TEM, dynamic light scattering, and ICP-OES techniques. Then, peptides-conjugated AuNPs were evaluated regarding the antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. The antitumoral activity was evaluated in vitro by cell viability assays with metastatic melanoma cell line (B16F10-Nex2) and the cytotoxicity was evaluated against human foreskin fibroblast (Hs68) cell line. Finally, in vivo assays were performed by using a syngeneic animal model of metastatic melanoma. Our findings have highlighted the potential application of the dual-peptide AuNPs in order to enhance the antitumor and antimicrobial activity of peptides.

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The efficiency of photothermal action of gold shell-isolated nanoparticles against tumor cells depends on membrane interactions

Cited by 8 publications

References 111 publications

Enhancing Phototoxicity in Human Colorectal Tumor Cells Through Nanoarchitectonics for Synergistic Photothermal and Photodynamic Therapies

Enhancing Phototoxicity in Human Colorectal Tumor Cells Through Nanoarchitectonics for Synergistic Photothermal and Photodynamic Therapies

Immunoassay platform with surface-enhanced resonance Raman scattering for detecting trace levels of SARS-CoV-2 spike protein

Co-Functionalization of Gold Nanoparticles with C7H2 and HuAL1 Peptides: Enhanced Antimicrobial and Antitumoral Activities

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