The Efficiency of Simian Foamy Virus Vector Type-1 (SFV-1) in Nondividing Cells and in Human PBLs

Abstract

Virology 280 (2001) 243-252. doi:10.1006/viro.2000.0773Received by publisher: 2000-09-05Harvest Date: 2016-01-04 12:20:53DOI: 10.1006/viro.2000.0773Page Range: 243-25

Gene therapy progress and prospects: Novel gene therapy approaches for AIDS

“…Early tries at anti-HIV-1 therapy in neurons has failed due to difficulty in gene transfer efficiency, 23 but this might be overcome in later attempts. Nonretroviral vectors such as Simian foamy virus (SFV-1)-based vectors have been used to transduce several chemically arrested cell lines and terminally differentiated human neurons, 56 and may become a vector of choice.…”

Development of Foamy Virus Vectors for Gene Therapy for Neurological Disorders and Other Diseases

“…Most retroviral vectors derived from FVs are nonpathogenic to humans (Caprariello et al, 2009). There is not enough evidence indicating the ability of foamy viruses to be transmitted between populations (Liu et al, 2005;Mergia et al, 2001). Furthermore, long-term studies of animal care workers and experimental researchers infected by foamy viruses have failed to show negative consequences (Mergia et al, 2001;Saib et al, 1997).…”

“…There is not enough evidence indicating the ability of foamy viruses to be transmitted between populations (Liu et al, 2005;Mergia et al, 2001). Furthermore, long-term studies of animal care workers and experimental researchers infected by foamy viruses have failed to show negative consequences (Mergia et al, 2001;Saib et al, 1997). Thus, there is little or no risk of developing malignancies in patients treated with gene therapy by FV vectors.…”

“…NT2.N neurons, a postmitotic human neuronal cell line, can be transduced by simian foamy virus-1 (SFV-1) vectors (Mergia et al, 2001). Neural cells, cultured astrocytes, cultured rat hippocampal and dorsal root ganglia neurons are also transducible by FV vectors (Liu et al, 2005;Liu et al, 2007;Liu et al, 2008).…”

“…) Foamy viral reverse transcription, integration, transcription and packaging processes are also distinct. The reverse transcription during viral particle formation produces 20% of viral particles containing the full-length viral cDNA genome (Mergia et al, 2001), which is conducive to the formation of the PIC. The Tas (transactivator of spumavirus) plays a critical role in foamy viral replication and transcription.…”